pCMV-ATP13A5-2×Strep-Tag II-Neo plasmid

PVT110885

pCMV-ATP13A5-2×Strep-Tag II-Neo plasmid Information

Plasmid name: pCMV-ATP13A5(human)-2×Strep-Tag II-Neo
Alias: NM_198505.4
Gene lenght: 3654
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ATP13A5-2×Strep-Tag II-Neo plasmid Description

pCMV-ATP13A5-2×Strep-Tag II-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-ATP13A5-2×Strep-Tag II-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CgccaccATG GAAGAGAACA GTAAGAAGGA CCATCGGGCT TTGCTCAACC AGGGAGAGGA 961 GGATGAACTG GAGGTGTTTG GTTACCGGGA CCACAATGTA CGGAAAGCCT TCTGCCTTGT 1021 CGCATCCGTG CTGACCTGTG GGGGCCTTCT GCTGGTGTTC TACTGGAGAC CCCAGTGGAG 1081 AGTGTGGGCC AACTGCATCC CATGCCCCTT GCAAGAAGCA GACACTGTTT TGCTGAGGAC 1141 AACAGACGAA TTTCAAAGAT ATATGAGGAA GAAGGTATTC TGCCTCTACT TATCCACACT 1201 GAAGTTTCCT GTAAGCAAGA AGTGGGAAGA ATCCCTGGTG GCTGACCGCC ACTCTGTCAT 1261 AAACCAAGCC TTAATAAAGC CAGAATTAAA ACTGCGGTGC ATGGAAGTGC AGAAAATCAG 1321 GTATGTTTGG AACGACCTGG AGAAGCGGTT TCAGAAAGTT GGGTTGCTAG AAGACAGCAA 1381 TTCCTGCTCT GACATCCATC AGACATTTGG ATTGGGTCTG ACCAGTGAAG AGCAAGAGGT 1441 CAGAAGATTA GTGTGTGGGC CCAACGCCAT TGAGGTTGAA ATCCAACCCA TATGGAAGCT 1501 GCTTGTTAAA CAGGTTTTAA ATCCATTCTA TGTGTTCCAA GCCTTCACCC TAACTTTGTG 1561 GCTGTCTCAA GGTTACATAG AATACTCTGT GGCCATCATC ATTTTGACTG TTATCTCCAT 1621 TGTCTTAAGT GTGTATGATT TGCGACAGCA ATCAGTTAAG CTGCATAACC TCGTGGAGGA 1681 CCACAACAAA GTCCAGGTTA CAATCATTGT AAAAGACAAA GGTTTGGAGG AGCTGGAATC 1741 CCGTCTCTTG GTTCCCGGAG ACATTCTTAT TCTTCCAGGA AAATTTTCAT TGCCATGTGA 1801 TGCTGTTTTG ATTGATGGAA GCTGCGTGGT GAATGAAGGC ATGCTTACAG GAGAAAGTAT 1861 ACCTGTTACA AAGACACCAT TGCCCCAGAT GGAGAACACT ATGCCTTGGA AATGTCACAG 1921 TTTGGAGGAT TATAGGAAAC ACGTCCTTTT CTGTGGAACA GAAGTTATCC AGGTCAAGCC 1981 CTCTGGGCAG GGGCCTGTAC GAGCAGTCGT TTTGCAAACA GGTTACAATA CAGCCAAAGG 2041 GGACTTAGTG AGATCCATCC TGTACCCCCG GCCTCTGAAC TTCAAACTAT ACAGCGATGC 2101 CTTCAAGTTC ATCGTGTTCC TGGCCTGCCT TGGTGTCATG GGTTTTTTCT ATGCCCTAGG 2161 GGTATATATG TACCATGGAG TTCCTCCAAA AGATACTGTG ACCATGGCCC TGATCCTCCT 2221 CACCGTGACT GTCCCTCCAG TGCTGCCAGC TGCCCTGACC ATAGGCAACG TGTATGCTCA 2281 GAAGAGACTG AAGAAAAAGA AAATCTTCTG TATCTCCCCA CAGAGAATCA ACATGTGTGG 2341 GCAAATAAAC CTCGTGTGCT TTGACAAAAC TGGCACTCTC ACTGAAGATG GGCTGGACCT 2401 CTGGGGGACT GTCCCTACTG CTGACAACTG CTTCCAGGAA GCCCACAGCT TTGCCTCAGG 2461 CCAGGCTGTG CCATGGAGCC CACTGTGTGC GGCCATGGCC AGCTGCCACT CTCTGATCCT 2521 TCTCAATGGG ACCATCCAGG GAGACCCTCT GGACCTCAAA ATGTTTGAGG GCACTGCCTG 2581 GAAAATGGAA GATTGCATTG TAGACTCCTG CAAATTTGGG ACGTCAGTTT CAAACATCAT 2641 AAAACCAGGA CCAAAAGCCA GTAAGAGTCC AGTGGAAGCC ATCATCACCT TGTGCCAGTT 2701 TCCATTTTCC TCGAGCCTGC AGAGGATGTC CGTGATCGCT CAGCTAGCTG GGGAGAATCA 2761 TTTCCATGTC TACATGAAAG GTGCCCCAGA AATGGTGGCC AGGTTCTGCA GATCTGAAAC 2821 AGTGCCCAAG AATTTCCCAC AGGAACTGAG GAGTTACACG GTGCAAGGCT TCCGTGTCAT 2881 TGCTCTTGCC CACAAAACCT TAAAGATGGG GAATCTTTCA GAAGTCGAGC ACTTAGCCAG 2941 AGAAAAAGTG GAGTCAGAGT TAACATTTCT GGGACTTCTC ATCATGGAGA ATCGCTTGAA 3001 AAAAGAAACC AAACTGGTCT TGAAGGAACT GAGTGAGGCC CGTATCAGGA CTGTGATGAT 3061 TACAGGTGAT AACCTTCAAA CGGCCATTAC TGTTGCAAAG AATTCTGAAA TGATCCCTCC 3121 AGGCAGCCAA GTGATCATTG TTGAGGCCGA TGAACCAGAA GAATTTGTTC CTGCCTCTGT 3181 GACCTGGCAG CTGGTGGAGA ACCAAGAGAC TGGACCTGGG AAGAAAGAAA TCTACATGCA 3241 TACTGGAAAC AGTTCAACCC CTCGTGGGGA AGGAGGAAGC TGTTACCATT TTGCAATGAG 3301 TGGGAAATCA TACCAAGTGA TATTTCAGCA TTTCAACAGC TTGCTTCCAA AAATTCTGGT 3361 GAATGGAACA GTTTTTGCAA GAATGTCTCC TGGGCAGAAA TCAAGCCTTA TTGAAGAATT 3421 TCAGAAATTA AATTATTATG TGGGCATGTG TGGAGATGGA GCTAACGACT GTGGGGCTTT 3481 GAAAGCGGCT CATGCAGGCA TTTCATTATC AGAGCAGGAA GCATCTGTGG CATCCCCTTT 3541 TACCTCTAAA ACAACCAACA TCCAGTGTGT GCCTCATCTC ATCAGAGAAG GCCGAGCTGC 3601 TCTGGTTTCA TCCTTTGGAG TATTTAAATA CTTGACCATG TACGGCATAA TCCAGTTTAT 3661 CAGTGCATTA CTGCTCTATT GGCAACTACA ACTCTTTGGA AATTACCAGT ATCTCATGCA 3721 AGATGTAGCC ATTACTTTGA TGGTCTGTTT AACAATGAGT TCAACTCATG CCTACCCAAA 3781 GCTGGCTCCA TATAGACCAG CAGGACAGCT CCTTTCTCCC CCTTTACTGC TTTCAATATT 3841 TTTGAATTCC TGTTTCTCCT GCATTGTGCA GATCAGTGCA TTTCTCTATG TGAAGCAGCA 3901 GCCTTGGTAT TGTGAGGTCT ACCAATACAG TGAGTGTTTT CTGGCCAACC AAAGTAATTT 3961 TTCAACAAAT GTGAGTTTGG AAAGAAACTG GACTGGAAAT GCAACTCTGA TTCCTGGTTC 4021 AATTTTAAGT TTTGAGACCA CCACACTGTG GCCCATCACC ACCATCAACT ATATCACAGT 4081 AGCATTCATC TTTTCTAAGG GAAAGCCATT TCGAAAACCC ATCTATACAA ACTATATATT 4141 TTCATTTCTG CTGCTAGCTG CCTTGGGCCT CACAATTTTC ATTCTGTTTT CTGACTTTCA 4201 AGTTATATAC CGTGGAATGG AGTTGATCCC AACCATAACA TCGTGGAGGG TTTTAATTTT 4261 GGTGGTAGCC CTCACCCAAT TCTGTGTGGC TTTCTTTGTA GAGGATTCCA TCCTTCAAAA 4321 TCATGAACTC TGGCTGTTGA TCAAAAGAGA ATTTGGATTC TACTCTAAAA GTCAATATAG 4381 GACTTGGCAA AAGAAGCTAG CAGAAGACTC AACCTGGCCT CCCATAAACA GGACAGATTA 4441 TTCAGGTGAT GGCAAAAATG GATTCTACAT CAACGGAGGC TATGAAAGCC ATGAACAGAT 4501 TCCAAAAAGA AAACTCAAAT TGGGAGGCCA ACCCACAGAA CAGCATTTCT GGGCCAGGCT 4561 GTGGAGCCAT CCGCAGTTTG AAAAAGGCGC CATGACCGGT TGGTCCCACC CGCAGTTCGA 4621 AAAATAACTC GAGTCTAGAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC 4681 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 4741 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 4801 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 4861 TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG 4921 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 4981 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 5041 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT 5101 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 5161 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 5221 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 5281 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 5341 GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 5401 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 5461 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5521 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 5581 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 5641 GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 5701 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 5761 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 5821 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 5881 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 5941 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 6001 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 6061 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 6121 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 6181 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 6241 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 6301 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 6361 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 6421 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 6481 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 6541 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 6601 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 6661 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 6721 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 6781 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 6841 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 6901 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 6961 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 7021 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 7081 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 7141 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 7201 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 7261 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 7321 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 7381 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 7441 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 7501 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 7561 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 7621 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 7681 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 7741 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 7801 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 7861 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 7921 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 7981 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 8041 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 8101 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 8161 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 8221 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 8281 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 8341 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 8401 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 8461 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 8521 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 8581 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 8641 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 8701 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 8761 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 8821 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 8881 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 8941 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 9001 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 9061 CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.