pCMV-CCDC15-3×FLAG-Neo plasmid

PVT108916

pCMV-CCDC15-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-CCDC15(human)-3×FLAG-Neo
Alias: NM_025004.3
Gene lenght: 2853
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-CCDC15-3×FLAG-Neo plasmid Description

pCMV-CCDC15-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CCDC15-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgct gggaagtatg gcccgaaaga 781 aacctcgaaa tacctcaagg ttgcccctgg ctttaaaccc cctgaagagc aaggacgtgt 841 tggcagtgct ggctgagagg aacgaggcta tagtaccagt tggggcatgg gtggaacctg 901 cctcaccagg tagttcggaa atcccagcat atacatcagc atatttaatt gaagaagaac 961 taaaggaaca gctaagaaaa aaacaagaag ctttgaaaca ttttcagaaa caagttaaat 1021 accgagtaaa tcaacaaatt aggttgagaa aaaagcaaca gcttcagaag tcttatgaaa 1081 gagcacaaaa agaaggctcc atagccatgc agtcttcagc aacacactta acttccaaaa 1141 ggacaagtgt ttttccaaac aatttgaatg ttgctattgg aagttctagg ttacctcctt 1201 ccctgatgcc tggggatgga atagaggatg aagagaatca gaacgaatta ttccaacaac 1261 aagcccaggc tcttagtgaa actatgaaac aggcacgtca ccggctagca tcctttaaaa 1321 ccgtgattaa aaaaaaggga tcagtgtttc cagatgatgg aaggaaaagc tttcttacca 1381 gagaggaagt gctttccagg aaaccagcat ccactgggat aaatacagga ataagaggag 1441 agttgcccat taaggtccat caaggtcttt tagctgctgt accttaccag aattatatgg 1501 aaaatcagga acttgactat gaggaacctg actatgagga atcttcatct cttgtaactg 1561 atgagaaagg gaaagaagat ttgtttggga gaggccagca ggaccagcag gctatccatt 1621 ctgaagataa gaacaaacct ttcagcagag ttcagaaagt aaaattcaaa aatccattat 1681 ttgttctgat ggaagaggaa gaacaaaagc agttacattt tgagggcctt caggatattc 1741 tgccagaagc ccaggattat tttctagaag cccaaggtga tttgctggaa acccagggtg 1801 atttgacagg aatccagagt gttaagccag atacccaggc tgttgaaatg aaggttcagg 1861 ttactgagcc agaaggccag gccattgagc cagaaggcca gcctattaag acagaaactc 1921 agggtattat gctgaaagcc cagagtattg agctagaaga agggagtatt gtgttgaaaa 1981 cccaggattt tctacccaca aatcaggctc ttctaacgaa aaaccaggat gttttactca 2041 aagaccactg tgttctccct aaagaccaga gtattctact caaatatcag gaccaggact 2101 tcctacccag agaccagcat gttctccaca aagaccaaga tattctgcca aaatatcagg 2161 accagaattt tctacctaag gaccagaatt ttttatctag agaccagcat gttctcccca 2221 aagaccaaga tattctgcca aaatatcagg accagaattt tctacctaag gaccagaatt 2281 ttttgtctag agaccagcat gttctcccca aagaccagaa tattctacct aaatatcaag 2341 gccaggattt tctacctaaa gaccaggact ttttatctag agaccagcat gttctcccca 2401 aagactggaa tattctaccc aaatgtcagg accaggattt tctacccaga gaccaaggtg 2461 ttcttcccaa agaccaaaat attctaccca tatgtcagga ccaggatttt ctacccagag 2521 accaaggtta tcttcctaaa gaccaaaata ttctacccat atgtcaggac cgggattttc 2581 tacccagaga cctgcatgtt ctctccaacg accagaatat tctacccaaa tgtcaggacc 2641 aagattttct accaaaatat cagaaagtac actttaagga gccatactct gatatgacag 2701 atgagaaagg gagagaagac ttttctctgg cagactatca gtgtttgcct cccaaatccc 2761 aggaccagga tgacatcaaa aatcagcaac ctgcatcttt tatgagagaa gaaagagtga 2821 gagaagaatt gcctctggac tatcatcaat atgttgtacc taaaatccag gaccaagact 2881 cccctagaga acagaacaag catatcaaac taccctcatc ttttgagaaa tgggagattg 2941 caagaggaaa tactcctgga gtgcccttgg cttatgatag gtatcaatca ggattgagca 3001 ctgaattcca agctccactg gcatttcagt ctgacgtgga taaagaagaa gataagaaag 3061 agcgtcaaaa gcagtacctg agacatagac gacttttcat ggatattgag agagaacaag 3121 ttaaagaaca acaaaggcaa aaagaacaaa agaagaaaat tgaaaaaatt aagaaaaaga 3181 gagagcaaga atgttatgct gcagagcaga ggatcctaag aatgaacttt catgaagatc 3241 catattcagg agagaagttg agtgagatat tagcccagtt acaacttcaa gaaataaaag 3301 gaaccagaga aaaacaacag agagaaaaag aatacctgag atatgtagaa gctttacgag 3361 cccaaatcca ggagaaaatg cagctgtata atattacttt acctccacta tgctgttgtg 3421 gtcctgattt ttgggatgct catcctgata cctgtgccaa caactgtatt ttctataaaa 3481 accacagagc atatactcgg gcactacatt cattcatcaa ttcctgtgat gtccctgggg 3541 gtaattcaac tcttcgagtc gcaattcata attttgcttc tgcacacagg cggactttga 3601 aaaatctaGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT 3661 ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA 3721 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 3781 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 3841 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 3901 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 3961 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 4021 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 4081 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG 4141 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 4201 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 4261 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA 4321 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA 4381 ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC 4441 TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC 4501 ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG 4561 CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 4621 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 4681 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 4741 TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 4801 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 4861 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 4921 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG 4981 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 5041 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 5161 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 5221 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 5281 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 5341 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 5401 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 5461 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 5521 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 5581 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 5641 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 5701 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 5761 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 5821 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 5881 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 5941 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 6001 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 6061 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 6121 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 6181 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 6241 GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA 6301 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 6361 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT 6421 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 6481 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 6541 TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 6601 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 6661 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 6721 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 6781 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 6841 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 6901 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 6961 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 7021 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 7081 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 7141 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA 7201 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 7261 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 7321 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 7381 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 7441 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 7501 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 7561 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 7621 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 7681 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 7741 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 7801 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 7861 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 7921 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 7981 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8041 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8101 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8161 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8221 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8281 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8341 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8401 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 8461 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 8521 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 8581 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 8641 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 8701 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 8761 CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 8821 TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT 8881 ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.