pCMV-Chd8-3×FLAG-Neo plasmid

PVT111370

pCMV-Chd8-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Chd8(mouse)-3×FLAG-Neo
Alias: NM_201637.3;5830451P18Rik; Chd-8; Duplin; HELSNF1;mKIAA1564
Gene lenght: 7746
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Chd8-3×FLAG-Neo plasmid Description

pCMV-Chd8-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Chd8-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatggc agaccccatc atggatcttt 781 ttgatgaccc aaacttattt ggcctggact ctctgactga tgacagcttt aatcaggtca 841 ctcaagaccc cattgaagaa gcacttggac tgccaagctc tctggactcc ttggatcaga 901 tgaaccaaga tggtggaggt ggtgatgtgg ggaattcatc agcaagtgac ctggttcctc 961 caccagagga aacagcttcc actgaacttc ccaaagaatc cacagctcca gctccagaat 1021 ccttaacctt gcatgattat accactcagc ccaccagcca ggagcagcca gcccagcctg 1081 tcttgcagac atcaacacca acagcaggac tcttgcaggt ctccaagagc caggagatct 1141 tgagccaagg gaatcctttc atgggtgtct ctgccacagg tgtctccccc agtaatactg 1201 gaggacaacc atctcagtca gctcctaaga ttgttattct taaagctcca ccaaactcct 1261 ccgtcacagg cacccatgtg gcacaaattc aggcccaagg tatcaccagt acagctcagc 1321 ccctagtggc tgggacagcc aacggtggaa aagtcacttt taccaaagtg ctgactggta 1381 caccccttcg accaggtgta tccattgtct ctggtaatac agtgttggcc acgaaggtcc 1441 ctgggaacca ggctgctgtt cagcgcattg tccagccaag ccgaccagta aaacagctgg 1501 tcctccagcc agtaaagggt tcagctcctg ctgggaatcc tggggctgcc gggcccccac 1561 tgaagcctgc agttacactg acgtctacac ctacccaggg tgaatcgaaa cgcatcactt 1621 tggtccttca acagccacag tccggaggtc cccaaggaca tcggcatgtt gtgttaggga 1681 gtctaccagg caagatagtg ttacagggca accagctagc agccttgact caagccaaga 1741 atgcccaggg gcagcctgcc aaagtagtaa ctattcagct gcaggtgcag cagccacagc 1801 agaaaatcca gattgtacct cagcctccat catcacaacc acagccacag ccgcagccgc 1861 caccctcagc ccagccgttg accctgtcct ctgtgcagca ggctcagata atggggccag 1921 gccaaaaccc agggcagaga ctttctgtac cgctcaagat ggtgctgcag ccacaggctg 1981 gctcttctca gggagcctct tctggactct ctgtggttaa agttctaagt gccagtgaag 2041 tggcagccct gtcatcccca gcaagctgtg cccctcatac tgcagggaag actgggatgg 2101 aggagaaccg caggttggag caccagaaaa agcaagagaa agcaaatcgg attgtggcag 2161 aggccattgc tagggcccgg gccaggggcg agcaaaacat acctcgagtc ctgaatgagg 2221 atgagctgcc tagtgtccgg ccagaggagg agggtgaaaa gaaacgcagg aagaagagca 2281 gtggggaaag gctgaaggag gagaagccaa agaagagcaa gactgctgct gcctccaaaa 2341 cgaagggcaa gagtaagcta aacaccatca ctcctgtagt gggcaagaag agaaagcgta 2401 acacatcgtc tgacaactct gacgtggaag ttatgcctgc acaatcgccc cgggaggacg 2461 aagagagcag catccagaag agacgttcaa accgccaagt taagcgaaaa aaatatacag 2521 aggacctgga tataaagata acagacgatg aagaggagga ggaggtcgat gtaactggtc 2581 caataaaacc cgagcccatc ctcccagaac cagtgcaaga gcctgatggc gagactcttc 2641 cttccatgca gttctttgtg gagaatccta gtgaagaaga tgcagctatt gtggacaaag 2701 tgctttccat gcgagttgtg aagaaagagc ttccttctgg acaatacact gaggctgaag 2761 aattcttcgt caagtacaag aactactctt atctgcactg tgagtgggca acgatctccc 2821 agctggagaa ggataagagg atccaccaga aactaaaacg gttcaaaaca aaaatggctc 2881 agatgagaca cttctttcat gaagatgaag agcctttcaa tccagactac gtagaggtgg 2941 ataggatact ggacgagtct cacagtgttg acaaggataa tggtgagcct gtaatttact 3001 acctggtaaa atggtgctct ctgccctatg aggacagtac gtgggagcta aaagaggatg 3061 ttgatgaggg caagattcgg gaatttaaac ggatccagtc aaggcaccca gaactgagaa 3121 gggtgaatcg tccacaggca aatgcctgga agaaattgga gttatcacat gaatataaaa 3181 acagaaacca attacgggaa tatcagttag aaggggtcaa ctggctcctc tttaattggt 3241 ataacaggca gaactgcatc ctggctgatg aaatgggatt gggcaaaact attcagtcaa 3301 tcgccttctt gcaggaggta tataatgtag gcatccatgg tccctttttg gtcattgctc 3361 cattgtccac cattactaac tgggagcgtg aattcaatac atggacagag atgaacacta 3421 ttgtgtacca tggcagcctg gccagccggc agatgatcca gcagtatgaa atgtactgta 3481 aagactcacg gggtcgcctc atccctggtg catataagtt tgatgccttg atcaccactt 3541 ttgagatgat tttgtcagac tgtcctgagc ttcgtgaaat tgaatggcgt tgtgttatca 3601 ttgatgaagc ccataggctg aagaaccgta attgcaagct acttgatagt ctcaagcaca 3661 tggacctgga gcataaagtg ttactcacag ggacaccgtt acaaaatacc gtagaggaac 3721 tgttcagtct actgcatttc ttggaacctt ctcagttccc ctcagaatca gaattcctta 3781 aggattttgg ggatctcaag accgaggagc aggttcaaaa gcttcaggcc attctaaagc 3841 caatgatgct gaggagactc aaagaggatg ttgaaaaaaa tttggctccc aaacaggaaa 3901 ctattattga agtagagcta accaacatcc agaagaaata ctacagagct attttagaga 3961 agaatttctc cttcctttcc aaaggggcag gtcataccaa tatgcctaat ctactgaaca 4021 caatgatgga gctacgaaag tgctgcaatc acccgtacct catcaatggt gcagaagaga 4081 aaatcctgat ggaatttcgg gaagcttgcc atattatacc tcaagatttc cacctgcagg 4141 ctatggttcg gtcagctggc aagttagttc tcattgacaa gttacttcca aagcttaaag 4201 ctggtggcca taaagttctg atcttctccc agatggtacg ctgtctagac attctggagg 4261 attatctgat ccagaggagg tacttatatg aacgtattga tgggcgagtt agaggcaacc 4321 ttcgacaagc tgctatcgac cgctttagca agcctgactc agaccgcttt gtcttcttgc 4381 tatgtacccg tgctggtgga cttggtatta atcttacagc tgctgatacc tgtattatct 4441 ttgattcaga ctggaatcca cagaatgacc tgcaggccca agcacgttgt catcgaattg 4501 gacagagcaa agctgtgaag gtgtaccgac tcatcactcg taattcctat gagagagaga 4561 tgtttgataa agctagcctc aagttgggat tggataaagc tgtgcttcag tccatgagtg 4621 gtcgggatgg caacattact ggaattcaac agttctccaa gaaagagatt gaagatctct 4681 taaggaaagg tgcctatgcg gccataatgg aggaagacga tgagggttct aagttttgtg 4741 aggaggacat agatcagatc ttactgagac ggaccacaac catcactatt gagtctgaag 4801 ggaaaggttc tacttttgcc aaggcaagct ttgttgcttc tgaaaatagg acagatattt 4861 ccctggatga tccaaacttt tggcaaaaat gggccaaaaa ggcagaccta gacatggatc 4921 tgctgaatag taagaataac ttagtgatcg acacacctcg agttcggaaa cagactcgac 4981 acttcagcac tttgaaagat gatgacctgg ttgagttttc tgatttggaa agtgaagatg 5041 atgaacggcc acgttcccga agacatgacc ggcatcacac ctatgggcgc actgactgct 5101 tccgggtaga aaagcatctc ctggtatatg gttggggacg ttggagagat attttgtctc 5161 acggacgatt taagcggcgc atgactgaaa gagatgttga gacaatatgc cgtgccatcc 5221 tcgtgtactg tcttctacac taccgtgggg atgaaaacat caaaagcttc atttgggact 5281 tgattagccc tgctgaaaat ggcaagacaa aagaattgca gaatcattca ggtctgtcta 5341 tccctgtgcc ccgtgggcgt aaggggaaaa aagtcaagtc acaaagtact tttgacattc 5401 ataaggcaga ctggatccgg aaatacaacc ctgatactct gttccaagat gagagttata 5461 agaagcattt gaaacatcag tgtaacaagg tactgttgag agtccggatg ctctactacc 5521 tgagacagga ggttattgga gaccaggcag agaaggtgtt aggaggtgcc atagccagtg 5581 agattgacat atggttccca gtggtggatc agctggaggt tcctacaact tggtgggata 5641 gtgaggctga caaatccctg ctcattggcg tttttaagca tggctatgag aaatacaata 5701 ccatgagggc agaccctgcc ttgtgcttcc tagaaaaggc tggcaggcca gatgacaaag 5761 ccatcgcagc agaacataga gtgttggata atttctccga cctggtagaa gggattgact 5821 ttgataagga ttgtgaagat cctgaatata aacccctcca gggtcctcca aaggacccag 5881 atgatgaggg tgatcccttg atgatgatgg atgaggagat ctcagtcatc gacggagagg 5941 aagcccaggt aactcaacag ccagggcatt tattctggcc tccaggctcc gccctcacag 6001 ctaggcttcg ccgcctagta acagcctatc aacgcagcta caagagagaa caaatgaaga 6061 tggaggctgc ggaacgtggg gaccggagac gccgacgatg tgaagcagcc tttaagctca 6121 aagaaatcgc aaggcgggaa aaacaacaac ggtggacaag gcgtgagcaa actgatttct 6181 accgagtagt atctaccttt ggtgtggagt atgaccctga caacatgcag tttcactggg 6241 atcgcttccg cacttttgct cgcctggaca aaaaaacaga tgaaagcctt accaagtact 6301 tccatggttt tgtggccatg tgtcgacaag tgtgccgcct tcccccagct gctggtgatg 6361 aacctccaga ccctaatctg ttcattgagc ccatcactga agagagagcc tcgaggactc 6421 tctaccgaat tgagttgctt cgacgcttac gggaacaagt tttatgccac ccccttttag 6481 aagatcggct ggcattgtgt cagcctccag gtcttgaatt gcccaaatgg tgggaacctg 6541 tccgtcatga tggggagctc ctacgaggag cagcccgcca tggggtgagc caaacagact 6601 gcaacatcat gcaggaccca gacttctctt ttctggctgc ccgtatgaat tatatgcaga 6661 accatcaggc gggagcatca gctgcatccc tgtcgcgatg ctccactcca ctgctgcacc 6721 agcagtgtac ctcacgtact gcttcaccat cacccctgcg cccagatgct cctgttgaaa 6781 agtcacctga ggaaagtact gttcaggtcc ccaatctgga gagtctgact ttaaagttag 6841 aggatgaggt tgtggctagg agccgactca cctcacaaga ctatgaagta cgcgtaggct 6901 cttcagatac agctcctctg tcccggagtg tcccaccagt gaaactggag gacgaggatg 6961 attcagactc tgagctggac ttgagcaagt tgtcaccatc ctcctcctct tcttcatcct 7021 cgtccagctc cagctccagc actgacgaga gtgaagacga gaaggaagag aagttaactg 7081 ctgaccggtc ccgcccaaag ctctatgatg aagagagcct tctgtccctt actatgtccc 7141 aagatggatt cccaaatgaa gatggagaac aaatgacccc tgagttgctg ctactgcagg 7201 aaagacaaag agcctctgaa tggcctaagg atcgtgttct gataaaccgc attgacctcg 7261 tctgccaggc tgtactctca gggaaatggc cttctaaccg ccggagccag gaagtgacag 7321 caggaggaat tttggggcca ggcaaccatt tattagacag tccctctttg accccaggag 7381 aagatgggga ctctccagtc cccacgccac gaagtggcag tgcagcttcc atggcggagg 7441 aagaggcatc tgcagtcacc acagcggcgg cccagtttac gaaacttcgg cgaggcatgg 7501 atgagaaaga gtttacagtt cagatcaaag atgaagaagg attgaagtta acattccaga 7561 agcatagatt gatggctaat ggtgtaatgg gagatggaca tccactgttt cataagaaga 7621 aggggaacag gaaaaagcta gtagagctgg aggtggagtg catggaagag cctaatcacc 7681 ttgatctgga cctggagacc cggatccctg tcatcaataa ggtggatggt actttgctgg 7741 tgggtgatga ggcccctcgc cgggcggagc tggagatgtg gttacagggt catccagagt 7801 ttgctgttga tccccgattt ctagcgtata tggaggaacg tagaaaacag aagtggcaga 7861 gatgtaaaaa aaataataag gcagaattga actgtttggg aatggaacca gtacagccag 7921 cgaactctag aaatgggaaa aagggtcact atgctgaaac tgcgttcaac cgggttttgc 7981 cagggcctgt tgcaccagag aacagcaaga aacgggtccg taggacaaga ccagaccttt 8041 ctaagatgat ggccctgatg cagggtggaa gcactgggtc cctgtctctg cataacacct 8101 ttcaacacag cagtagtaac ctacagtctg tgtcgtctct gggtcacagc agtaccactt 8161 ctgcatctct gccttttatg ccgtttgtga tgggtgcagc agcaccccct catgtagact 8221 ccagcaccat gcttcatcat caccaccacc atccccaccc ccaccatcac caccatcacc 8281 atccaggctt gagaaccact ggctaccctt cttcaccagc tactaccacc tctggtactg 8341 ccttgcggtt accaacactg caacctgagg acgatgatga agaggaggat gaagaagatg 8401 atgatttatc tcagggctat gacagctcag aacgggactt ctcactcatt gatgacccta 8461 tgatgccagc caactcagac tccagtgaag atgctgacga cGGATCCGAC TACAAAGACC 8521 ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG 8581 GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 8641 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 8701 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 8761 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 8821 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 8881 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 8941 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC 9001 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 9061 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 9121 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 9181 CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC 9241 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA 9301 TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG 9361 GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG 9421 CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG 9481 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 9541 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 9601 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT 9661 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 9721 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 9781 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 9841 GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA 9901 GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 9961 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 10021 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 10081 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 10141 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 10201 GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 10261 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 10321 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 10381 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 10441 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 10501 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 10561 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 10621 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 10681 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 10741 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 10801 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 10861 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 10921 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 10981 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 11041 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 11101 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC 11161 CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA 11221 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA 11281 TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 11341 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 11401 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA 11461 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 11521 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 11581 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 11641 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 11701 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 11761 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 11821 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 11881 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 11941 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 12001 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 12061 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 12121 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 12181 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 12241 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 12301 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 12361 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 12421 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 12481 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 12541 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 12601 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 12661 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 12721 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 12781 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 12841 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 12901 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 12961 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 13021 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 13081 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 13141 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 13201 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 13261 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 13321 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 13381 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 13441 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 13501 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 13561 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 13621 AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT 13681 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG 13741 GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT 13801 TGCATGAAGA ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.