pCMV-Cog1-3×FLAG-Neo plasmid

PVT111047

pCMV-Cog1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Cog1(mouse)-3×FLAG-Neo
Alias: NM_013581.3;6030445I15; Ldlb; mKIAA1381
Gene lenght: 2940
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Cog1-3×FLAG-Neo plasmid Description

pCMV-Cog1-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Cog1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatggc ggccgcgacc gcctcctccg 781 cgctcaagcg gctggatctg cgcgatccca acgcgctctt cgagactcat ggagcggagg 841 agatccgcgg gctggagcgc caggtccgag ccgagatcga acacaagaag gaggagctgc 901 ggcagatggt gggcgagcgg taccgcgacc tgatcgaggc ggcggacacc atcggccaga 961 tgcgccgctg cgccgagggc ctggtggacg ccgtgcaggc caccgatcaa tactgcgccc 1021 gcctccgcca ggccggctcc gtggcgcccc gggtcccacg ggccccgcag ccacagccgc 1081 cgtccgagaa gttctacagc atggccgccc agattaagct gctgctggag atcccggaga 1141 agatctggag cgccatggaa gcctcgcagc acctccaggc cacacagctc tacctgctct 1201 gctgccacct gcatagcctg ctgcagctgg actcctctaa ctccagatac agccccatcc 1261 tctcgagatt tccaatcctc atccgccagg tggcagcagc cagccacttc cggtcaacta 1321 ttttgcacga aagcaagatg ctgctcaaat gccaggctgt gtcggaccag gctgtggccg 1381 aagccctctg ctccataatg ctcttggagg agagctctcc gcgccaagcc ctcacagact 1441 tcctgctggc cagaaaggcg actattcaga cgctcctgaa ccagtctcat cacggtgctg 1501 gcatcaaggc ccagatttgc tctttggtgg agctgctggc taccactctg aaccaggcac 1561 atgccctttt ctacacccta ccagaaggcg tgctgcccga cccatccctg ccctgtggct 1621 tgctcttttc taccctggag acagtcacaa ggcagcatcc cactggaaag ggcatcggtg 1681 ccctccaagg ggagatgaag ctatgcagct ggttcagaca cctgcccacc tccattatcg 1741 agttccagcc cacactccga actctcgcac atcccatcag ccaggagtac ctgaaagaca 1801 cgctgcagaa atggatcgac atgtgcaatg aagacattaa aaatgggatc ggcaatctgc 1861 ttatgtacgt gaagagcatg aagggtctgg caggcatcag agacgccata tgggatttgc 1921 ttagcaacga gtctgccagc cacagctggg aggtggtgtg tcagcggctt ctggagaagc 1981 cgctcttgtt ctgggaggac ctgatgcagc agctcttcct ggatcggtta cagacactga 2041 ccagagaagg ctttgaatcc atctccaaca gctccaaaga gcttctagtc tcagctctgc 2101 aggagctgga gaccaacaac tccacatcga acaagcatgt ccactttgag cagaacatgt 2161 ctttcttcct ctggtccgag agccccaacg acctgccttc tgatgctgcc tgggtcagcg 2221 tagcaaaccg ggctcagttt gccagtagtg gcctctccat gaaagcccaa gccattagcc 2281 cgtgtgtcca gaacttctgt tctgccctgg attctaagct gaaggttaaa ctggacgacc 2341 tcctggccta ccttccctcc agtgacacac cattgctcaa ggataccacc cccacgcacc 2401 agcctaagaa ctctgccttt gatcgatacg cagatgctgg gaccgtgcag gacatgctgc 2461 gtacgcagtc cgtggcctgc atcaagtccg tcgtgggctg catccaggca gagctgtgca 2521 ccattgaaga agtcacacgg gaacaaaagg atgtccttca cagcaccaag ctgcacgcag 2581 tcctcttcat ggccagactc tgccagtctc tgggagagct gtgccctcac ctaaagcagt 2641 gtgttgtggg acagtgcggg ggttctgaga aacctgccag ggaggcgagg gctctgaaaa 2701 agcaggggaa ggggagagct caggatgtcc tccccgcgca ggcccagtgg cagggagtga 2761 aggaagtcct gctccaacag agcgtcatgg cctaccgggt ctggagcaca gcacttgtga 2821 aatttctgat ttgtggattc actcgatcac tgcttctcag ggatgccggc tcagtcctgg 2881 ccactgccac caattgggat gaactagaaa ttcaagagga gacagagtct ggcagcagtg 2941 tcacgtctaa gatccgcctc cctactcagc cctcctggta tgtacagtcc ttcctgttta 3001 gcttatgcca ggaagttaat cgggttggag gccatgcttt gccaaaggtc accctacagg 3061 agatgctgaa gacctgcatg gcccaagtca tagctgccta tgagcaactc acagaggaaa 3121 accagataaa gaaagaaggg gcatttccaa tgacccagaa ccgagccctg cagctcctct 3181 acgacctacg ctacctcacc atggttctga gcagcaaggg ggaggaggtg aagagtggcc 3241 ggagcaaggc agactccaga atggagaaga tgaccgagag gctggaggcc ctcattgacc 3301 cttttgacct ggatgtcttc acaccgcatc tcaacagcaa cctcaaccgc ctggtgcagc 3361 ggacttcagt tctatttggc ttggttacag gcacagagaa tcagtttgcc tcccggagca 3421 gcactttcaa ctcccaggag ccccataaca tactgccgct ggcctccagt cagatcaggt 3481 ttggactcct tccactgagc atgacaagca caagaaaggc gagggcaacc agtagaagcg 3541 ttgaaaccca agcccaggtt ggccccccgg cactctccag agtaggtgac ccgacgacgc 3601 atcctggctc cttattcagg cagcttgcaa gcgaggaaga cgactcacct gcaccttcat 3661 tattcaagct tgcctggctc tctagtatga ccaagGGATC CGACTACAAA GACCATGACG 3721 GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG 3781 ATATCTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 3841 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT 3901 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA 3961 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC 4021 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT 4081 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG 4141 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC 4201 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC 4261 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT 4321 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC 4381 TGGAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT 4441 ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG 4501 CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA 4561 ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG 4621 GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC 4681 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 4741 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 4801 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT 4861 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 4921 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 4981 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 5041 TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 5101 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 5161 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 5221 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 5281 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 5341 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 5401 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 5461 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 5521 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 5581 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 5641 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 5701 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 5761 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 5821 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 5881 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 5941 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 6001 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 6061 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 6121 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 6181 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 6241 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 6301 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGCGAAA TGACCGACCA 6361 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 6421 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 6481 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 6541 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 6601 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 6661 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 6721 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 6781 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 6841 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 6901 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 6961 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 7021 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 7081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 7141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 7201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 7261 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 7321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 7381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 7441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 7501 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 7561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 7621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 7681 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 7741 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 7801 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 7861 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 7921 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 7981 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 8041 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 8101 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 8161 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 8221 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 8281 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 8341 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 8401 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 8461 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 8521 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 8581 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 8641 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 8701 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 8761 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 8821 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC 8881 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT 8941 GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG 9001 AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.