pCMV-CTCF-3×FLAG-Puro plasmid

PVT110595

pCMV-CTCF-3×FLAG-Puro plasmid Information

Plasmid name: pCMV-CTCF(human)-3×FLAG-Puro
Alias: NM_006565.4;CFAP108; FAP108; MRD21
Gene lenght: 2181
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-CTCF-3×FLAG-Puro plasmid Description

pCMV-CTCF-3×FLAG-Puro It is a plasmid suitable for gene expression in mammalian cells.

pCMV-CTCF-3×FLAG-Puro plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCgc caccatggaa ggtgatgcag tcgaagccat tgtggaggag 961 tccgaaactt ttattaaagg aaaggagaga aagacttacc agagacgccg ggaagggggc 1021 caggaagaag atgcctgcca cttaccccag aaccagacgg atgggggtga ggtggtccag 1081 gatgtcaaca gcagtgtaca gatggtgatg atggaacagc tggaccccac ccttcttcag 1141 atgaagactg aagtaatgga gggcacagtg gctccagaag cagaggctgc tgtggacgat 1201 acccagatta taactttaca ggttgtaaat atggaggaac agcccataaa cataggagaa 1261 cttcagcttg ttcaagtacc tgttcctgtg actgtacctg ttgctaccac ttcagtagaa 1321 gaacttcagg gggcttatga aaatgaagtg tctaaagagg gccttgcgga aagtgaaccc 1381 atgatatgcc acaccctacc tttgcctgaa gggtttcagg tggttaaagt gggggccaat 1441 ggagaggtgg agacactaga acaaggggaa cttccacccc aggaagatcc tagttggcaa 1501 aaagacccag actatcagcc accagccaaa aaaacaaaga aaaccaaaaa gagcaaactg 1561 cgttatacag aggagggcaa agatgtagat gtgtctgtct acgattttga ggaagaacag 1621 caggagggtc tgctatcaga ggttaatgca gagaaagtgg ttggtaatat gaagcctcca 1681 aagccaacaa aaattaaaaa gaaaggtgta aagaagacat tccagtgtga gctttgcagt 1741 tacacgtgtc cacggcgttc aaatttggat cgtcacatga aaagccacac tgatgagaga 1801 ccacacaagt gccatctctg tggcagggca ttcagaacag tcaccctcct gaggaatcac 1861 cttaacacac acacaggtac tcgtcctcac aagtgcccag actgcgacat ggcctttgtg 1921 accagtggag aattggttcg gcatcgtcgt tacaaacaca cccacgagaa gccattcaag 1981 tgttccatgt gcgattacgc cagtgtagaa gtcagcaaat taaaacgtca cattcgctct 2041 catactggag agcgtccgtt tcagtgcagt ttgtgcagtt atgccagcag ggacacatac 2101 aagctgaaaa ggcacatgag aacccattca ggggaaaagc cttatgaatg ttatatttgt 2161 catgctcggt ttacccaaag tggtaccatg aagatgcaca ttttacagaa gcacacagaa 2221 aatgtggcca aatttcactg tccccactgt gacacagtca tagcccgaaa aagtgatttg 2281 ggtgtccact tgcgaaagca gcattcctat attgagcaag gcaagaaatg ccgttactgt 2341 gatgctgtgt ttcatgagcg ctatgccctc atccagcatc agaagtcaca caagaatgag 2401 aagcgcttta agtgtgacca gtgtgattac gcttgtagac aggagaggca catgatcatg 2461 cacaagcgca cccacaccgg ggagaagcct tacgcctgca gccactgcga taagaccttc 2521 cgccagaagc agcttctcga catgcacttc aagcgctatc acgaccccaa cttcgtccct 2581 gcggcttttg tctgttctaa gtgtgggaaa acatttacac gtcggaatac catggcaaga 2641 catgctgata attgtgctgg cccagatggc gtagaggggg aaaatggagg agaaacgaag 2701 aagagtaaac gtggaagaaa aagaaagatg cgctctaaga aagaagattc ctctgacagt 2761 gaaaatgctg aaccagatct ggacgacaat gaggatgagg aggagcctgc cgtagaaatt 2821 gaacctgagc cagagcctca gcctgtgacc ccagccccac cacccgccaa gaagcggaga 2881 ggacgacccc ctggcagaac caaccagccc aaacagaacc agccaacagc tatcattcag 2941 gttgaagacc agaatacagg tgcaattgag aacattatag ttgaagtaaa aaaagagcca 3001 gatgctgagc ccgcagaggg agaggaagag gaggcccagc cagctgccac agatgccccc 3061 aacggagacc tcacgcccga gatgatcctc agcatgatgg accggCTCGA GACCGGTGAC 3121 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ATTACAAGGA TGACGATGAC 3181 AAGTGACCGG TaAGTTTAAA CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA 3241 TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC 3301 CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG 3361 GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT 3421 GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG 3481 TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 3541 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 3601 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 3661 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 3721 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 3781 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 3841 GATTTATAAG GGATTTTGGC CATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 3901 AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 3961 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 4021 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 4081 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 4141 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG 4201 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 4261 AGCTCCCggg atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 4321 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 4381 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 4441 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 4501 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 4561 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 4621 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 4681 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 4741 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 4801 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 4861 cggtgcctga TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 4921 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 4981 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 5041 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 5101 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 5161 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 5221 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 5281 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 5341 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 5401 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 5461 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 5521 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 5581 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 5641 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 5701 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 5761 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 5821 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 5881 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 5941 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 6001 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 6061 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 6121 AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 6181 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 6241 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 6301 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 6361 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 6421 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 6481 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 6541 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 6601 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 6661 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 6721 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 6781 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 6841 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 6901 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 6961 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 7021 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 7081 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 7141 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 7201 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 7261 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 7321 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.