pCMV-EGFP-Cacna1a-Neo plasmid

PVT109652

pCMV-EGFP-Cacna1a-Neo plasmid Information

Plasmid name: pCMV-EGFP-Cacna1a(mouse)-Neo
Alias: NM_007578.3;alpha1A; APCA; BI; Caca1a; Cacnl1a4;Cav2.1; Ccha1a; EA2; FHM; HPCA; la; MHP; MHP1; nmf352;rkr; SCA6; smrl; tg
Gene lenght: 7107
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-Cacna1a-Neo plasmid Description

pCMV-EGFP-Cacna1a-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-Cacna1a-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT Catggcccgc tttggagacg agatgccggg ccgctacggc 1681 gcaggcggag gaggctcagg gccggccgcc ggggtggtcg tgggcgccgc gggcggccga 1741 ggagccgggg gcagccggca gggcgggcag cccggagcgc agaggatgta caagcagtcg 1801 atggcgcaga gagcgcggac catggccctc tacaacccca tccctgtccg ccagaactgc 1861 ctcacggtca accgctccct gttcctcttc agtgaagaca acgtggtgag aaaatacgcc 1921 aaaaagatca ctgaatggcc tcccttcgag tacatgatcc tggccaccat cattgctaac 1981 tgcatcgtcc tggccctgga gcagcacctc cctgatgatg acaagacacc catgtctgag 2041 cggctggatg acacggaacc atacttcatt ggaatcttct gttttgaggc tggaattaag 2101 atcgtggctc ttggctttgc cttccacaaa ggctcctacc tgaggaatgg ctggaacgtc 2161 atggactttg tcgtggtgct aacaggcatc ttggccactg tcgggacgga gtttgaccta 2221 cggacactga gggcggttcg tgtgctgcgg ccactcaagc tggtgtctgg aatcccaagt 2281 ttacaagtcg tcctgaagtc aatcatgaag gcgatgatcc ctctgctgca gatcggcctc 2341 ctcctgtttt ttgcaatcct tatttttgca atcatagggt tagaatttta tatggggaaa 2401 tttcatacca cctgctttga agaggggaca gacgacatcc agggtgagtc gccagctccg 2461 tgtgggacag aggagcctgc ccgcacctgc cccaacggga ccaaatgtca gccgtactgg 2521 gaagggccca acaacggcat cactcagttc gacaacatcc tgtttgctgt gctcactgtt 2581 ttccagtgca tcaccatgga aggctggact gatctcctct acaatagcaa cgatgcctca 2641 gggaacactt ggaactggtt gtacttcatc cccctcatca tcatcggctc cttttttatg 2701 ctgaaccttg ttctgggtgt gctgtctggg gagtttgcca aagaaaggga acgtgtggag 2761 aaccggagag cttttctgaa gctcagaaga caacagcaga ttgaacgtga gctcaatgga 2821 tacatggagt ggatctcaaa agcagaagag gtgattcttg cggaggacga gacagacgtg 2881 gagcagaggc acccttttga tggagctctt cggagagcta ctctgaagaa aagcaagaca 2941 gacctgctca accctgagga ggccgaggac cagctggctg acatcgcgtc tgtggggtct 3001 cccttcgcca gagccagcat caaaagtgcc aagctggaga actcgacttt ttttcacaag 3061 aaggagagaa gaatgcgttt ctacatccgc cgcatggtca aaactcaggc cttctactgg 3121 accgtgctca gtctggtagc cctcaacacg ctgtgcgtcg ccattgtcca ctacaaccag 3181 cccgagtggc tctccgactt cctctactat gcagaattca ttttcttagg actctttatg 3241 tccgaaatgt ttataaaaat gtacgggctc gggacgcggc cttacttcca ctcttccttc 3301 aactgctttg actgtggggt catcattggg agcatctttg aagtcatctg ggctgtcatc 3361 aaacctggta catcctttgg aatcagtgtg ttacgagctc tcaggttatt gcgaattttc 3421 aaagtcacaa agtactgggc atctctcaga aacctggttg tctccctcct caactccatg 3481 aagtccatca taagtctgtt gttcctcctt ttcctgttca tcgttgtctt tgccctcctg 3541 gggatgcagc tgttcggtgg ccaatttaat tttgatgagg ggactccgcc caccaacttc 3601 gacacttttc cagcagcaat aatgactgtg tttcagatcc tgactggcga ggattggaat 3661 gaggtcatgt atgatgggat caagtctcag gggggcgtgc agggcggcat ggtgttctcc 3721 atctacttca tcgtcctcac cctcttcggg aactacaccc tgctgaacgt gttcttggcc 3781 atcgcagtgg acaacctggc caatgcccag gagctcacca aggatgagca agaagaggaa 3841 gaggcagcca atcagaagct ggctctacag aaagccaagg aggtagcaga agtgagtccc 3901 ctgtctgcag ccaacatgtc catagccgtg aaggagcagc agaagaacca gaagcctacc 3961 aagtcggtgt gggagcagcg caccagcgag atgcgcaagc agaacctgct ggccagccgc 4021 gaggcgctgt acggggacgc ggctgagcgc tggcccaccc cctacgcgcg cccgctgcgg 4081 cccgacgtga agactcactt ggaccggccg cttgtggtgg acccgcagga gaaccgtaac 4141 aacaacacca acaagagccg tgcgccagaa gcgctgcgcc cgaccgcgcg gccccgcgaa 4201 agcgcgcgcg accccgacgc gcggcgcgcc tggcccggca gccctgagcg cgcccctgga 4261 cgagagggcc cgtatgggcg cgagagcgag cctcagcagc gcgagcacgc gccgccccgc 4321 gagcacgcac cctgggacgc ggacaccgag cgcgccaaag ccggggacgc gccccgccgc 4381 cacacgcacc gacctgtggc ggagggcgag cctcgccgcc accgcgcccg ccgccggccc 4441 ggggacgaac ccgacgacag gccagagcgc aggccacgtc cccgcgacgc cactaggccg 4501 gcccgcgctg cagacggcga aggcgacgat ggggagcgca agcggcgaca ccgacacggg 4561 ccgccggccc acgacgacag ggagcgcaga caccgacgca gaaaagagaa ccagggctct 4621 ggggtccccg tgtctggtcc caacttgtcc accaccaggc caatccagca ggacctgggc 4681 cgccaggacc tgccgctggc tgaggatctt gacaacatga agaataacaa gctggccacc 4741 ggggaacctg ccagtcccca cgacagcctg ggccacagcg gccttccccc cagccccgcc 4801 aaaatcggga acagcaccaa ccctggtccc gccttggcca ccaatcccca gaacgctgcc 4861 agccgcagga cgcccaacaa tccgggcaac ccgtccaacc ccggcccccc caagactccc 4921 gagaacagcc ttatcgtcac caaccccagc agcacccagc ccaactcagc gaagactgcc 4981 aggaaacccg agcacatggc ggtggagatc cccccagcct gcccgcccct caaccacacc 5041 gtggtccaag taaacaaaaa cgccaaccca gacccactgc caaagaaaga ggaagagaag 5101 aaagaggaag aggaggcaga ccccggggag gacggcccaa agcccatgcc gccctacagc 5161 tccatgttca tcctctccac caccaacccc cttcgccggc tgtgccatta catcctgaac 5221 ctgcgctact tcgagatgtg catcctcatg gtcattgcca tgagcagcat cgctctggcc 5281 gccgaggacc cggtgcagcc caacgcaccc cgcaacaatg tgctgcgata ttttgactat 5341 gtttttacag gcgtgtttac ctttgagatg gtgatcaaga tgatcgacct gggcctcgtc 5401 ttgcatcagg gggcctattt ccgtgaccta tggaacattc tggacttcat agtggtcagt 5461 ggggccctgg tggcctttgc cttcactggc aatagcaaag gaaaggacat taacaccatt 5521 aagtctctcc gagtcctccg ggtgctacga cctctaaaga ccatcaagcg gctgccaaag 5581 ctgaaggccg tgtttgactg cgtggtgaac tcgctcaaga acgtcttcaa catcctcatt 5641 gtgtacatgc tgttcatgtt catcttcgct gtggtagccg tgcagctctt caagggcaag 5701 ttcttccact gcacggacga gtccaaggag ttcgagagag actgtcgagg caagtacctc 5761 ctgtacgaga agaacgaagt gaaggcccgg gaccgcgagt ggaagaagta cgagttccac 5821 tacgacaacg tgctctgggc tctgctcaca cttttcacag tgtccacagg agagggctgg 5881 ccacaggtcc tcaagcactc agtggatgct acttttgaga accagggccc cagccccggg 5941 taccgcatgg agatgtccat cttctacgtg gtgtactttg tagtgtttcc cttcttcttt 6001 gtcaatatct tcgtggcctt gatcatcatc acctttcagg agcagggaga caagatgatg 6061 gaagagtaca gcctggagaa aaatgagagg gcctgcatcg actttgccat cagtgccaag 6121 ccgctgacca ggcacatgcc ccagaacaag cagagcttcc agtatcgaat gtggcagttc 6181 gtcgtgtccc caccctttga gtacaccatc atggccatga tcgctctcaa caccatcgtg 6241 ctaatgatga agttctacgg agcctcggtg gcctatgaaa acgcccttcg cgtgttcaac 6301 attgtcttca cctccctctt ctctctcgaa tgtgtgctca aagtcatggc ttttgggatt 6361 ctgaattatt tccgcgatgc ctggaacatc ttcgactttg tgactgttct gggcagcatc 6421 acagacatcc tcgtcaccga gtttgggaat aacttcatca acctgagctt tctccgcctc 6481 ttccgtgctg ccagactcat caaactcctc cgccagggtt acaccatccg cattctcctc 6541 tggactttcg tgcagtcctt caaggcccta ccttatgtct gtctgctgat cgccatgctc 6601 ttcttcatct acgccatcat cgggatgcag gtgtttggca acatcggcat tgacggggaa 6661 gacgaggaca gcgacgagga tgaattccaa atcacggagc acaataactt ccggaccttc 6721 ttccaagcgc tcatgcttct cttccggagc gccacaggag aagcgtggca caacatcatg 6781 ctctcctgcc tcagcgggaa gccgtgcgat aagaactctg ggatcctcac ggcagactgc 6841 ggcaacgagt tcgcgtactt ctactttgtc tcgttcatct tcctgtgctc gtttctgatg 6901 ctgaatctct ttgtcgctgt catcatggac aacttcgagt acctcacccg agattcttcc 6961 atcctgggcc cccatcactt ggatgaatac gtgcgtgtct gggcagagta tgaccctgct 7021 gcctgcggcc gcattcacta taaggacatg tacagtttat tgcgagtaat atcgccccct 7081 ctcggcttag gcaagaaatg tcctcatagg gttgcttgca agagactcct gcggatggac 7141 ctgccggtag cagatgacaa cacagttcac ttcaactcca ccctgatggc tctgatccga 7201 accgcccttg atatcaaaat tgccaagggt ggagctgaca agcagcagat ggatgcagag 7261 cttcgcaagg agatgatggc catttggccc aacctgtctc agaagaccct ggatctgctg 7321 gtcacacctc acaagtccac ggacctgaca gtgggtaaga tctacgcagc catgatgatc 7381 atggagtatt accggcagag caaggccaag aaactgcagg ccatgcgaga ggagcagaac 7441 cggacaccac tcatgttcca gcgcatggag cctccatcac caacacagga gggaggaccc 7501 agccagaatg cccttccctc cactcagctg gacccaggag gaggcctgat ggctcacgaa 7561 ggcggcatga aggagagccc gtcctgggtg acccagcggg cccaagagat gttccagaag 7621 actggcacct ggagcccaga gcgagggcca cccatcgaca tgcctaacag ccagcccaac 7681 tcccagtctg tggagatgcg agaaatgggt actgatggct actcagacag cgaacactac 7741 ctccccatgg aaggccagac cagggccgcc tccatgcccc gcctcccagc agagaaccag 7801 aggagaaggg gccggccacg tggaaatgac ctcagtacca tctctgatac cagccccatg 7861 aagcgctcag cctctgtgct gggacccaaa gcccggcgac tggatgacta ctcactagag 7921 cgggtaccgc ctgaggagaa ccaacggtac caccaacgtc gccgggaccg tggccaccgc 7981 acctctgagc gctctctggg ccgatacact gatgtggaca caggtttggg gacagatctg 8041 agcatgacca cccaatcggg tgacctgccc tccaaagatc gggaccagga ccggggccga 8101 cccaaggacc ggaagcatcg gccacaccac caccaccacc accatcatca tcatcccccg 8161 gcccccgacc gggatcgcta cgcacaggag cggccggaca ccggccgggc gcgggcccgg 8221 gagcagcgct ggtcccgctc gcccagcgaa ggtcgggagc acacgacaca cagacagggc 8281 agtagttccg tcagtggaag cccagccccc tctacgtccg gcaccagcac gccgcggcgc 8341 ggccgccgcc agctccccca gaccccctgc accccgcggc cgcttgtgtc ctactcgccc 8401 gctccgcgca ggcctgcggc gcgcaggatg gcgggtcccg cagcgccccc tggcggctcg 8461 ccgcggggtt gccgccgtgc gccgcgctgg cctgcgcatg cgcctgaggg cccgcggccg 8521 cgcggtgccg actacaccga gcccgacagc ccgcgcgagc cgccaggggg cgcgcacgat 8581 cccgcgccgc ggtcgcccag gactccgcgc gccgcgggct gcgcgtcacc gcgccacggc 8641 cgcaggctgc ccaatggtta ctacgcgggg cacggcgcgc cgcggccgcg cacagcccgc 8701 aggggcgcgc acgacgcgta cagcgagagc gaggacgact ggtgctaaGG ATCCtGATAT 8761 CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCGTTT AAACCCGCTG ATCAGCCTCG 8821 ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC 8881 CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT 8941 CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT 9001 TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA 9061 AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG 9121 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 9181 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 9241 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 9301 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 9361 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 9421 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 9481 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC 9541 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 9601 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 9661 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC 9721 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 9781 ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT 9841 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 9901 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT 9961 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 10021 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 10081 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 10141 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 10201 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG 10261 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 10321 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 10381 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT 10441 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG 10501 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC 10561 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG 10621 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT 10681 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT 10741 CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC 10801 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 10861 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 10921 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 10981 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC 11041 CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 11101 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 11161 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 11221 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 11281 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 11341 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 11401 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 11461 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 11521 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 11581 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 11641 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 11701 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 11761 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 11821 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 11881 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 11941 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 12001 TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 12061 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 12121 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 12181 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 12241 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 12301 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 12361 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 12421 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 12481 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 12541 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 12601 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 12661 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 12721 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 12781 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 12841 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 12901 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 12961 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 13021 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 13081 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 13141 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 13201 CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.