pCMV-EGFP-HSP90B1-Neo plasmid

PVT109643

pCMV-EGFP-HSP90B1-Neo plasmid Information

Plasmid name: pCMV-EGFP-HSP90B1(human)-Neo
Alias: NM_003299.3;ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1
Gene lenght: 2409
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-HSP90B1-Neo plasmid Description

pCMV-EGFP-HSP90B1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-HSP90B1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT CATGAGGGCC CTGTGGGTGC TGGGCCTCTG CTGCGTCCTG 1681 CTGACCTTCG GGTCGGTCAG AGCTGACGAT GAAGTTGATG TGGATGGTAC AGTAGAAGAG 1741 GATCTGGGTA AAAGTAGAGA AGGATCAAGG ACGGATGATG AAGTAGTACA GAGAGAGGAA 1801 GAAGCTATTC AGTTGGATGG ATTAAATGCA TCACAAATAA GAGAACTTAG AGAGAAGTCG 1861 GAAAAGTTTG CCTTCCAAGC CGAAGTTAAC AGAATGATGA AACTTATCAT CAATTCATTG 1921 TATAAAAATA AAGAGATTTT CCTGAGAGAA CTGATTTCAA ATGCTTCTGA TGCTTTAGAT 1981 AAGATAAGGC TAATATCACT GACTGATGAA AATGCTCTTT CTGGAAATGA GGAACTAACA 2041 GTCAAAATTA AGTGTGATAA GGAGAAGAAC CTGCTGCATG TCACAGACAC CGGTGTAGGA 2101 ATGACCAGAG AAGAGTTGGT TAAAAACCTT GGTACCATAG CCAAATCTGG GACAAGCGAG 2161 TTTTTAAACA AAATGACTGA AGCACAGGAA GATGGCCAGT CAACTTCTGA ATTGATTGGC 2221 CAGTTTGGTG TCGGTTTCTA TTCCGCCTTC CTTGTAGCAG ATAAGGTTAT TGTCACTTCA 2281 AAACACAACA ACGATACCCA GCACATCTGG GAGTCTGACT CCAATGAATT TTCTGTAATT 2341 GCTGACCCAA GAGGAAACAC TCTAGGACGG GGAACGACAA TTACCCTTGT CTTAAAAGAA 2401 GAAGCATCTG ATTACCTTGA ATTGGATACA ATTAAAAATC TCGTCAAAAA ATATTCACAG 2461 TTCATAAACT TTCCTATTTA TGTATGGAGC AGCAAGACTG AAACTGTTGA GGAGCCCATG 2521 GAGGAAGAAG AAGCAGCCAA AGAAGAGAAA GAAGAATCTG ATGATGAAGC TGCAGTAGAG 2581 GAAGAAGAAG AAGAAAAGAA ACCAAAGACT AAAAAAGTTG AAAAAACTGT CTGGGACTGG 2641 GAACTTATGA ATGATATCAA ACCAATATGG CAGAGACCAT CAAAAGAAGT AGAAGAAGAT 2701 GAATACAAAG CTTTCTACAA ATCATTTTCA AAGGAAAGTG ATGACCCCAT GGCTTATATT 2761 CACTTTACTG CTGAAGGGGA AGTTACCTTC AAATCAATTT TATTTGTACC CACATCTGCT 2821 CCACGTGGTC TGTTTGACGA ATATGGATCT AAAAAGAGCG ATTACATTAA GCTCTATGTG 2881 CGCCGTGTAT TCATCACAGA CGACTTCCAT GATATGATGC CTAAATACCT CAATTTTGTC 2941 AAGGGTGTGG TGGACTCAGA TGATCTCCCC TTGAATGTTT CCCGCGAGAC TCTTCAGCAA 3001 CATAAACTGC TTAAGGTGAT TAGGAAGAAG CTTGTTCGTA AAACGCTGGA CATGATCAAG 3061 AAGATTGCTG ATGATAAATA CAATGATACT TTTTGGAAAG AATTTGGTAC CAACATCAAG 3121 CTTGGTGTGA TTGAAGACCA CTCGAATCGA ACACGTCTTG CTAAACTTCT TAGGTTCCAG 3181 TCTTCTCATC ATCCAACTGA CATTACTAGC CTAGACCAGT ATGTGGAAAG AATGAAGGAA 3241 AAACAAGACA AAATCTACTT CATGGCTGGG TCCAGCAGAA AAGAGGCTGA ATCTTCTCCA 3301 TTTGTTGAGC GACTTCTGAA AAAGGGCTAT GAAGTTATTT ACCTCACAGA ACCTGTGGAT 3361 GAATACTGTA TTCAGGCCCT TCCCGAATTT GATGGGAAGA GGTTCCAGAA TGTTGCCAAG 3421 GAAGGAGTGA AGTTCGATGA AAGTGAGAAA ACTAAGGAGA GTCGTGAAGC AGTTGAGAAA 3481 GAATTTGAGC CTCTGCTGAA TTGGATGAAA GATAAAGCCC TTAAGGACAA GATTGAAAAG 3541 GCTGTGGTGT CTCAGCGCCT GACAGAATCT CCGTGTGCTT TGGTGGCCAG CCAGTACGGA 3601 TGGTCTGGCA ACATGGAGAG AATCATGAAA GCACAAGCGT ACCAAACGGG CAAGGACATC 3661 TCTACAAATT ACTATGCGAG TCAGAAGAAA ACATTTGAAA TTAATCCCAG ACACCCGCTG 3721 ATCAGAGACA TGCTTCGACG AATTAAGGAA GATGAAGATG ATAAAACAGT TTTGGATCTT 3781 GCTGTGGTTT TGTTTGAAAC AGCAACGCTT CGGTCAGGGT ATCTTTTACC AGACACTAAA 3841 GCATATGGAG ATAGAATAGA AAGAATGCTT CGCCTCAGTT TGAACATTGA CCCTGATGCA 3901 AAGGTGGAAG AAGAGCCCGA AGAAGAACCT GAAGAGACAG CAGAAGACAC AACAGAAGAC 3961 ACAGAGCAAG ACGAAGATGA AGAAATGGAT GTGGGAACAG ATGAAGAAGA AGAAACAGCA 4021 AAGGAATCTA CAGCTGAAAA AGATGAATTG TAGGATCCtG ATATCCAGCA CAGTGGCGGC 4081 CGCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT 4141 GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC 4201 CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT 4261 CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA 4321 GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT 4381 CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 4441 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 4501 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 4561 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 4621 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 4681 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 4741 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 4801 TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA 4861 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 4921 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 4981 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG 5041 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 5101 CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT 5161 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG 5221 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 5281 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 5341 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 5401 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 5461 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 5521 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 5581 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 5641 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 5701 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 5761 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 5821 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA 5881 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG 5941 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT 6001 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA 6061 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG 6121 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG 6181 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC 6241 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG 6301 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG 6361 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC 6421 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC 6481 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG 6541 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 6601 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 6661 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 6721 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 6781 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 6841 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 6901 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 6961 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 7021 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 7081 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 7141 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 7201 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 7261 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT 7321 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 7381 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 7441 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 7501 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 7561 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 7621 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 7681 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 7741 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 7801 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 7861 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 7921 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 7981 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 8041 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 8101 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 8161 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 8221 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 8281 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 8341 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 8401 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 8461 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 8521 GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.