pCMV-Gata4-3×FLAG-mCMV-EGFP plasmid

PVT94218

pCMV-Gata4-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-Gata4(mouse)-3×FLAG-mCMV-EGFP
Alias: NM_001310610.1;Gata-4
Gene lenght: 1326bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Gata4-3×FLAG-mCMV-EGFP plasmid Description

pCMV-Gata4-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-Gata4-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccatgtac caaagcctgg ccatggccgc 661 caaccacggc cccccgcccg gcgcctacga agcaggtggc cctggcgcct tcatgcacag 721 cgcgggcgcc gcgtcctcgc ccgtctacgt gcccactccg cgggtgccgt cctctgtgct 781 gggcctgtcc tacctgcagg gcggtggcag tgccgctgca gctggaacca cctcgggtgg 841 cagctccggg gccggcccgt cgggtgcagg gcctgggacc cagcagggta gccctggctg 901 gagccaagct ggagccgagg gagccgccta caccccgccg cccgtgtccc cgcgcttctc 961 tttcccgggg actactgggt ccctggcggc cgctgccgcc gctgccgcag cccgggaagc 1021 tgcagcctac ggcagtggcg gcggggcggc gggcgctggt ctggctggcc gagagcagta 1081 cgggcgtccg ggcttcgccg gctcctactc cagcccctac ccagcctaca tggccgacgt 1141 gggagcatcc tgggccgcag ccgctgccgc ctctgccggc cccttcgaca gcccagtcct 1201 gcacagcctg cctggacggg ccaaccctgg aagacacccc aatctcgtag atatgtttga 1261 tgacttctca gaaggcagag agtgtgtcaa ttgtggggcc atgtccaccc cactctggag 1321 gcgagatggg acgggacact acctgtgcaa tgcctgtggc ctctatcaca agatgaacgg 1381 catcaaccgg cccctcatta agcctcagcg ccgcctgtcc gcttcccgcc gggtaggcct 1441 ctcctgtgcc aactgccaga ctaccaccac cacgctgtgg cgtcgtaatg ccgagggtga 1501 gcctgtatgt aatgcctgcg gcctctacat gaagctccat ggggttccca ggcctcttgc 1561 aatgcggaag gaggggattc aaaccagaaa acggaagccc aagaacctga ataaatctaa 1621 gacgccagca ggtcctgctg gtgagaccct ccctccctcc agtggtgcct ccagcggtaa 1681 ctccagcaat gccactagca gcagcagcag cagtgaagag atgcgcccca tcaagacaga 1741 gcccgggctg tcatctcact atgggcacag cagctccatg tcccagacat tcagtactgt 1801 gtccggccac gggccctcca tccatccagt gctgtctgct ctgaagctgt ccccacaagg 1861 ctatgcatct cctgtcactc agacatcgca ggccagctcc aagcaggact cttggaacag 1921 cctggtcctg gctgacagtc atggggacat aatcaccgcg AAGCTTACGC GTGACTACAA 1981 AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTG 2041 AGGATCCGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC 2101 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 2161 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 2221 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 2281 TGGGGATGCG GTGGGCTCTA TGGTCTAGAG ATATACTGAG TCATTAGGGA CTTTCCAATG 2341 GGTTTTGCCC AGTACATAAG GTCAATAGGG GTGAATCAAC AGGAAAGTCC CATTGGAGCC 2401 AAGTACACTG AGTCAATAGG GACTTTCCAT TGGGTTTTGC CCAGTACAAA AGGTCAATAG 2461 GGGGTGAGTC AATGGGTTTT TCCCATTATT GGCACGTACA TAAGGTCAAT AGGGGTGAGT 2521 CATTGGGTTT TTCCAGCCAa TTTAATTAAA ACGCCATGTA CTTTCCCACC ATTGACGTCA 2581 ATGGGCTATT GAAACTAATG CAACGTGACC TTTAAACGGT ACTTTCCCAT AGCTGATTAA 2641 TGGGAAAGTA CCGTTCTCGA GCCAATACAC GTCAATGGGA AGTGAAAGGG CAGCCAAAAC 2701 GTAACACCGC CCCGGTTTTC CCCTGGAAAT TCCATATTGG CACGCATTCT ATTGGCTGAG 2761 CTGCGTTCTA CGTGGGTATA AGAGGCGCGA CCAGCGTCGG TACCGTCGCA GTCTTCGGTC 2821 TGACCACCGT AGAACGCAGA TCaccggtcg ccaccatggt gagcaagggc gaggagctgt 2881 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 2941 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 3001 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 3061 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 3121 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 3181 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 3241 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 3301 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 3361 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 3421 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 3481 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 3541 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat 3601 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 3661 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 3721 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 3781 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta 3841 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 3901 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 3961 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 4021 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 4081 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 4141 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 4201 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 4261 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 4321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4381 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 4441 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 4501 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 4561 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 4621 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 4681 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 4741 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 4801 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 4861 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 4921 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 4981 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 5041 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 5101 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 5161 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 5221 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 5281 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 5341 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 5401 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 5461 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 5521 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 5581 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 5641 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 5701 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 5761 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 5821 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 5881 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 5941 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 6001 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 6061 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 6121 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 6181 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 6241 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 6301 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 6361 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 6421 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 6481 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 6541 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 6601 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 6661 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 6721 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 6781 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 6841 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 6901 cgccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???