pCMV-GLG1-Neo plasmid

PVT90233

pCMV-GLG1-Neo plasmid Information

Plasmid name: pCMV-GLG1(human)-Neo
Alias: NM_012201.6;CFR-1; ESL-1; MG-160; MG160
Gene lenght: 3609bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GLG1-Neo plasmid Description

pCMV-GLG1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GLG1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gcggcgtgtg gacgtgtacg gaggatgttc cgcttgtcgg cggcgctgca 961 tctgctgctg ctattcgcgg ccggggccga gaaactcccc ggccagggcg tccacagcca 1021 gggccagggt cccggggcca actttgtgtc cttcgtaggg caggccggag gcggcggccc 1081 ggcgggtcag cagctgcccc agctgcctca gtcatcgcag cttcagcagc aacagcagca 1141 gcagcaacag caacagcagc ctcagccgcc gcagccgcct ttcccggcgg gtgggcctcc 1201 ggcccggcgg ggaggagcgg gggctggtgg gggctggaag ctggcggagg aagagtcctg 1261 cagggaggac gtgacccgcg tgtgccctaa gcacacctgg agcaacaacc tggcggtgct 1321 cgagtgcctg caggatgtga gggagcctga aaatgaaatt tcttcagact gcaatcattt 1381 gttgtggaat tataagctga acctaactac agatcccaaa tttgaatctg tggccagaga 1441 ggtttgcaaa tctactataa cagagattaa agaatgtgct gatgaaccgg ttggaaaagg 1501 ttacatggtt tcctgcttgg tggatcaccg aggcaacatc actgagtatc agtgtcacca 1561 gtacattacc aagatgacgg ccatcatttt tagtgattac cgtttaatct gtggcttcat 1621 ggatgactgc aaaaatgaca tcaacattct gaaatgtggc agtattcggc ttggagaaaa 1681 ggatgcacat tcacaaggtg aggtggtatc atgcttggag aaaggcctgg tgaaagaagc 1741 agaagaaaga gaacccaaga ttcaagtttc tgaactctgc aagaaagcca ttctccgggt 1801 ggctgagctg tcatcggatg actttcactt agaccggcat ttatattttg cttgccgaga 1861 tgatcgggag cgtttttgtg aaaatacaca agctggtgag ggcagagtgt ataagtgcct 1921 ctttaaccat aaatttgaag aatccatgag tgaaaagtgt cgagaagcac ttacaacccg 1981 ccaaaagctg attgcccagg attataaagt cagttattca ttggccaaat cctgtaaaag 2041 tgacttgaag aaataccggt gcaatgtgga aaaccttccg cgatcgcgtg aagccaggct 2101 ctcctacttg ttaatgtgcc tggagtcagc tgtacacaga gggcgacaag tcagcagtga 2161 gtgccagggg gagatgctgg attaccgacg catgttgatg gaagactttt ctctgagccc 2221 tgagatcatc ctaagctgtc ggggggagat tgaacaccat tgttccggat tacatcgaaa 2281 agggcggacc ctacactgtc tgatgaaagt agttcgaggg gagaagggga accttggaat 2341 gaactgccag caggcgcttc aaacactgat tcaggagact gaccctggtg cagattaccg 2401 cattgatcga gctttgaatg aagcttgtga atctgtaatc cagacagcct gcaaacatat 2461 aagatctgga gacccaatga tcttgtcgtg cctgatggaa catttataca cagagaagat 2521 ggtagaagac tgtgaacacc gtctcttaga gctgcagtat ttcatctccc gggattggaa 2581 gctggaccct gtcctgtacc gcaagtgcca gggagacgct tctcgtcttt gccacaccca 2641 cggttggaat gagaccagtg aatttatgcc tcagggagct gtgttctctt gtttatacag 2701 acacgcctac cgcactgagg aacagggaag gaggctctca cgggagtgcc gagctgaagt 2761 ccaaaggatc ctacaccagc gtgccatgga tgtcaagctg gatcctgccc tccaggataa 2821 gtgcctgatt gatctgggaa aatggtgcag tgagaaaaca gagactggac aggagctgga 2881 gtgccttcag gaccatctgg atgacttggt ggtggagtgt agagatatag ttggcaacct 2941 cactgagtta gaatcagagg atattcaaat agaagccttg ctgatgagag cctgtgagcc 3001 cataattcag aacttctgcc acgatgtggc agataaccag atagactctg gggacctgat 3061 ggagtgtctg atacagaaca aacaccagaa ggacatgaac gagaagtgtg ccatcggagt 3121 tacccacttc cagctggtgc agatgaagga ttttcggttt tcttacaagt ttaaaatggc 3181 ctgcaaggag gacgtgttga agctttgccc aaacataaaa aagaaggtgg acgtggtgat 3241 ctgcctgagc acgaccgtgc gcaatgacac tctgcaggaa gccaaggagc acagggtgtc 3301 cctgaagtgc cgcaggcagc tccgtgtgga ggagctggag atgacggagg acatccgctt 3361 ggagccagat ctatacgaag cctgcaagag tgacatcaaa aacttctgtt ccgctgtgca 3421 atatggcaac gctcagatta tcgaatgtct gaaagaaaac aagaagcagc taagcacccg 3481 ctgccaccaa aaagtattta agctgcagga gacagagatg atggacccag agctagacta 3541 caccctcatg agggtctgca agcagatgat aaagaggttc tgtccggaag cagattctaa 3601 aaccatgttg cagtgcttga agcaaaataa aaacagtgaa ttgatggatc ccaaatgcaa 3661 acagatgata accaagcgcc agatcaccca gaacacagat taccgcttaa accccatgtt 3721 aagaaaagcc tgtaaagctg acattcctaa attctgtcac ggtatcctga ctaaggccaa 3781 ggatgattca gaattagaag gacaagtcat ctcttgcctg aagctgagat atgctgacca 3841 gcgcctgtct tcagactgtg aagaccagat ccgaatcatt atccaggagt ccgccctgga 3901 ctaccgcctg gatcctcagc tccagctgca ctgctcagac gagatctcca gtctatgtgc 3961 tgaagaagca gcagcccaag agcagacagg tcaggtggag gagtgcctca aggtcaacct 4021 gctcaagatc aaaacagaat tgtgtaaaaa ggaagtgcta aacatgctga aggaaagcaa 4081 agcagacatc tttgttgacc cggtacttca tactgcttgt gccctggaca ttaaacacca 4141 ctgcgcagcc atcacccctg gccgcgggcg tcaaatgtcc tgtctcatgg aagcactgga 4201 ggataagcgg gtgaggttac agcccgagtg caaaaagcgc ctcaatgacc ggattgagat 4261 gtggagttac gcagcaaagg tggccccagc agatggcttc tctgatcttg ccatgcaagt 4321 aatgacgtct ccatctaaga actacattct ctctgtgatc agtgggagca tctgtatatt 4381 gttcctgatt ggcctgatgt gtggacggat caccaagcga gtgacacgag agctcaagga 4441 caggctacaa tacaggtcag agacaatggc ttataaaggt ttagtgtggt ctcaggatgt 4501 gacaggcagt ccagccTAGC TCGAGTCTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC 4561 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 4621 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 4681 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 4741 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA 4801 AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC 4861 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 4921 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 4981 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 5041 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 5101 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 5161 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 5221 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT 5281 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5341 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 5401 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 5461 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 5521 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 5581 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 5641 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 5701 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 5761 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 5821 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 5881 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 5941 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 6001 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 6061 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 6121 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 6181 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 6241 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 6301 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 6361 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 6421 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 6481 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 6541 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 6601 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 6661 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 6721 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 6781 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 6841 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 6901 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 6961 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 7021 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 7081 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 7141 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 7201 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 7261 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 7321 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 7381 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 7441 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 7501 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 7561 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 7621 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 7681 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 7741 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 7801 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 7861 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 7921 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 7981 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 8041 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 8101 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 8161 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 8221 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 8281 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 8341 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 8401 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 8461 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 8521 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 8581 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 8641 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 8701 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 8761 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 8821 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 8881 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 8941 CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.