pCMV-Guf1-3×FLAG-Neo plasmid

PVT101247

pCMV-Guf1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Guf1(mouse)-3×FLAG-Neo
Alias: NM_172711.3;4631409J12; EF-4; mtEF4
Gene lenght: 1953bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Guf1-3×FLAG-Neo plasmid Description

pCMV-Guf1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Guf1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgtg ggcgctcgtg gggcgggctc 781 tcgcgccgtg ggccgccggg gcccggcacg cggctgcctc ggagcccagg gccgcctgca 841 ggctcttcag cgcggccgag ctcaaggaga aacctgacat gtctagattt cctgttgaag 901 acattagaaa tttcagtatc attgcacatg tggatcatgg caaaagcact ttagctgaca 961 ggctcctgga gctaacagga acaattgata agacgaagaa aaacaagcag gttcttgata 1021 aattacaagt agaacgagaa agaggaatca ctgtgaaggc gcagacagcc tctctgtttt 1081 acagctttgg agggaaacag taccttttaa acctcatcga cacaccaggc cacgttgatt 1141 ttagttatga agtgtccagg tcactgtctg catgccaagg tgttttactg gtagtggatg 1201 caaatgaggg tattcaagct caaactgtgg ccaacttctt ccttgctttt gaagcacagc 1261 tgtcagtaat tccagttatc aataagatag atctgaagaa tgctgaccct gaaagggtgg 1321 gaaagcagat tgaaaaagta tttgatatcc ctagtgagga gtgtattaag atttctgcga 1381 aacttgggac aaacgttgac agtgtgcttc aggcagtcat tgaacgaata cctcctccaa 1441 aagtccatcg tgaaaaccca ctgaaagctt tggtatttga ctccaccttt gaccagtaca 1501 gaggggtgat agccaatata gcactgtttg atggagtggt ttccaaggga gataaaattg 1561 tatctgcaca tactaaaaaa gcatatgaag tcaatgaagt aggcattttg aatcctaatg 1621 aacagccaac tcataaatta tatgcaggac aggtgggctt tctgattgct gggatgaaag 1681 atgtcactga agcacaaata ggagacacat tatatttaca taatcatcca gtggaaccct 1741 tgcctgggtt taaatcagcg aaaccaatgg tgtttgcagg agtgtaccct atagaccaat 1801 ctgagtacaa taacctgaag agtgctatag aaaaactgac cttaaatgac tcaagtgtaa 1861 cagtgcatcg ggatagtagt ctggccctgg gtgctggctg gaggttgggg tttcttggac 1921 ttttacatat ggaggttttc aaccagcgat tagagcaaga atataacgct tctgtcattt 1981 tgacaacccc aactgttcca tacaaagctg ttctttcctc tgcaaaatta ataaaggaat 2041 ataaagagaa ggagatcaca atcatcaacc ctgcacagtt ccctgagaag tcccaagtaa 2101 cagaatattt ggaacctgtt gttttgggca ctgttattac accgactgag tacactggaa 2161 aaataatggc gctttgtcag gcacgaagag caattcagaa aaatatgact tttattgatg 2221 aaaatagagt tatgcttaaa tatctcttcc ctttgaatga aattgtggta gatttttatg 2281 actctttgaa gtctctctct tctggatatg caagttttga ttatgaagat gccggttacc 2341 agactgcaga actcgttaaa atggatattc tgctgaatgg aaatatggtg gaagagcttg 2401 taactgtggt ccatagagag aaagcctaca ctgttggcaa aagcatatgt gaacgcctga 2461 aggagtctct tcccagacaa ctgtatgaga tagcaattca agctgctgtt gggagcaaag 2521 ttattgcaag agaaacagtg aaagcctaca gaaaaaatgt tttggcaaaa tgttatggtg 2581 gtgatattac ccggaaaatg aaacttctga agagacagtc cgaagggaaa aagaagctga 2641 ggaagattgg caacattgag attccaaaag acgctttcat aaaagtcctg aaaacccagc 2701 ctaataaaGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT 2761 ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA 2821 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 2881 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 2941 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 3001 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 3061 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 3121 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 3181 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG 3241 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 3301 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 3361 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA 3421 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA 3481 ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC 3541 TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC 3601 ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG 3661 CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 3721 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 3781 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 3841 TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 3901 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 3961 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 4021 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG 4081 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 4141 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 4201 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 4261 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 4321 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 4381 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 4441 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 4501 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 4561 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 4621 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 4681 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 4741 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 4801 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 4861 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 4921 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 4981 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 5041 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 5101 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 5161 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 5221 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 5281 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 5341 GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA 5401 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 5461 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT 5521 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 5581 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 5641 TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 5701 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 5761 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 5821 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 5881 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 5941 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 6001 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 6061 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 6121 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 6181 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 6241 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA 6301 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 6361 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 6421 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 6481 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 6541 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 6601 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 6661 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 6721 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 6781 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 6841 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 6901 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 6961 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 7021 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 7081 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 7141 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 7201 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 7261 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 7321 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 7381 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 7441 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 7501 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 7561 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 7621 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 7681 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 7741 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 7801 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 7861 CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 7921 TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT 7981 ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???