pCMV-Hspa5-TurboID-Neo plasmid

PVT101487

pCMV-Hspa5-TurboID-Neo plasmid Information

Plasmid name: pCMV-Hspa5(mouse)-TurboID-Neo
Alias: NM_022310.3;baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;Hsce70; mBiP; SEZ-7; Sez7;
Gene lenght: 1965bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Hspa5-TurboID-Neo plasmid Description

pCMV-Hspa5-TurboID-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Hspa5-TurboID-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg atgaagttca ctgtggtggc ggcggcgttg ctgctgctgg gcgcggtgcg 961 ggccgaggag gaggacaaga aggaggatgt gggcacggtg gtcggcatcg acttggggac 1021 cacctattcc tgcgtcggtg tgttcaagaa cggccgcgtg gagatcatag ccaacgatca 1081 gggcaaccgc atcacgccgt cgtatgtggc cttcactcct gaaggggagc gtctgattgg 1141 cgatgcggcc aagaaccaac tcacgtccaa ccccgagaac acggtcttcg atgccaagcg 1201 cctcatcgga cgcacttgga atgacccttc ggtgcagcag gacatcaagt tcttgccatt 1261 caaggtggtt gaaaagaaaa ctaaaccgta cattcaagtt gatattggag gtgggcaaac 1321 caagacattt gccccagaag aaatttctgc catggttctc actaaaatga aggagactgc 1381 tgaggcgtat ttgggaaaga aggttaccca tgcagttgtt actgtaccag cttacttcaa 1441 tgatgcccag cgacaagcaa ccaaagatgc tggcactatt gctggactga atgtcatgag 1501 gatcatcaat gagcctacag cagctgctat tgcatatggc ctggataaga gagagggaga 1561 gaagaacatc cttgtgtttg acctgggtgg cggcaccttc gatgtgtctc ttctcaccat 1621 tgacaatggt gtctttgaag tggtggccac taatggagat actcacctgg gtggggaaga 1681 ctttgatcag cgggtcatgg aacacttcat caagttgtac aaaaagaaaa ctggtaaaga 1741 tgttaggaaa gacaacagag ctgtgcagaa actccggcgt gaggtagaaa aggctaagag 1801 agccttgtct tctcagcatc aagcaaggat tgaaattgag tccttcttcg aaggagaaga 1861 cttctcagag acccttactc gggccaaatt tgaagagctg aacatggacc tgttccgctc 1921 taccatgaag cctgtccaga aagtgttgga agactctgat ctgaagaaat ctgatattga 1981 tgaaattgtt ctggttggtg gatctactcg aattccaaag attcagcaac tggtgaaaga 2041 gttcttcaat ggcaaggagc catcccgtgg cataaacccc gatgaggctg tagcctatgg 2101 tgccgctgtc caggctggtg tcctctctgg tgatcaggat acaggtgatc tggtactgct 2161 tgatgtttgt ccccttacac ttggtattga aactgtggga ggagtcatga caaaactgat 2221 tccaaggaac actgtggtac ccaccaagaa gtctcagatc ttctccacgg cttccgataa 2281 tcagccaact gtaacaatca aggtctatga aggtgaacga cccctaacaa aagacaatca 2341 tcttctgggt acatttgatc tgactggaat tcctcctgct ccccgtggag ttccccagat 2401 tgaagtcact tttgagatag atgttaatgg tattctccga gtgacagctg aagacaaagg 2461 tacaggaaac aaaaacaaaa tcacaattac caatgaccaa aaccgcctga cacctgaaga 2521 aattgaaagg atggttaatg atgctgagaa gtttgctgag gaagacaaaa agctcaaaga 2581 gcgcattgac accaggaatg aattggaaag ctatgcttat tctctcaaga accagattgg 2641 agataaagaa aagctgggag gtaaactttc ttctgaagat aaagaaacca tggaaaaagc 2701 tgtagaggaa aagattgaat ggctggaaag ccaccaggat gcggacattg aagactttaa 2761 agccaaaaag aaggaactag aagaaattgt tcagccaatt atcagcaaac tctatggaag 2821 tggaggccct cccccaactg gtgaagagga tacatcagaa aaagatgagt tgGAATTCgg 2881 tggaggcgga tcaggaggcg gtggatctAA AGACAATACT GTGCCTCTGA AGCTGATCGC 2941 TCTCCTGGCT AATGGCGAGT TCCATAGTGG CGAACAGCTG GGAGAAACCC TGGGCATGTC 3001 CAGGGCCGCT ATCAACAAGC ACATTCAGAC TCTGCGCGAC TGGGGCGTGG ACGTGTTCAC 3061 CGTGCCCGGA AAGGGCTACT CTCTGCCCGA GCCTATCCCG CTGCTGAACG CTAAACAGAT 3121 TCTGGGACAG CTGGACGGCG GGAGCGTGGC AGTCCTGCCT GTGGTCGACT CCACCAATCA 3181 GTACCTGCTG GATCGAATCG GCGAGCTGAA GAGTGGGGAT GCTTGCATTG CAGAATATCA 3241 GCAGGCAGGG AGAGGAAGCA GAGGGAGGAA ATGGTTCTCT CCTTTTGGAG CTAACCTGTA 3301 CCTGAGTATG TTTTGGCGCC TGAAGCGGGG ACCAGCAGCA ATCGGCCTGG GCCCGGTCAT 3361 CGGAATTGTC ATGGCAGAAG CGCTGCGAAA GCTGGGAGCA GACAAGGTGC GAGTCAAATG 3421 GCCCAATGAC CTGTATCTGC AGGATAGAAA GCTGGCAGGC ATCCTGGTGG AGCTGGCCGG 3481 AATAACAGGC GATGCTGCAC AGATCGTCAT TGGCGCCGGG ATTAACGTGG CTATGAGGCG 3541 CGTGGAGGAA AGCGTGGTCA ATCAGGGCTG GATCACACTG CAGGAAGCAG GGATTAACCT 3601 GGACAGGAAT ACTCTGGCCG CTACGCTGAT CCGAGAGCTG CGGGCAGCCC TGGAACTGTT 3661 CGAGCAGGAA GGCCTGGCTC CATATCTGCC ACGGTGGGAG AAGCTGGATA ACTTCATCAA 3721 TAGACCCGTG AAGCTGATCA TTGGGGACAA AGAGATTTTC GGGATTAGCC GGGGGATTGA 3781 TAAACAGGGA GCCCTGCTGC TGGAACAGGA CGGAGTTATC AAACCCTGGA TGGGCGGAGA 3841 AATCAGTCTG CGGTCTGCCG AAAAGtgaCT CGAGTCTAGA GGGCCCGTTT AAACCCGCTG 3901 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 3961 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 4021 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 4081 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC 4141 TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC 4201 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 4261 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 4321 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 4381 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 4441 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 4501 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 4561 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG 4621 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 4681 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 4741 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 4801 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 4861 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA 4921 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 4981 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 5041 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 5101 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 5161 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 5221 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 5281 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 5341 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 5401 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 5461 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 5521 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 5581 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 5641 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 5701 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 5761 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 5821 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 5881 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 5941 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 6001 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 6061 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 6121 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 6181 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 6241 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 6301 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 6361 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 6421 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 6481 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 6541 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 6601 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 6661 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 6721 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 6781 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 6841 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 6901 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 6961 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 7021 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 7081 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 7141 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 7201 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 7261 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 7321 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 7381 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 7441 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 7501 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 7561 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 7621 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 7681 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 7741 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 7801 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 7861 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 7921 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 7981 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 8041 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 8101 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 8161 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 8221 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 8281 GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT 8341 ATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???