pCMV-KDM6A-3×FLAG-Blast plasmid

PVT111240

pCMV-KDM6A-3×FLAG-Blast plasmid Information

Plasmid name: pCMV-KDM6A(human)-3×FLAG-Blast
Alias: NM_021140.4;bA386N14.2; KABUK2; UTX
Gene lenght: 4203
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: KDM6A-F1
Reverse primer:
Copy number: High
Induction:

pCMV-KDM6A-3×FLAG-Blast plasmid Description

pCMV-KDM6A-3×FLAG-Blast It is a plasmid suitable for gene expression in mammalian cells.

pCMV-KDM6A-3×FLAG-Blast plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCga tatcGGATCC GCCACCatga aatcctgcgg agtgtcgctc 961 gctaccgccg ccgctgccgc cgccgctttc ggtgatgagg aaaagaaaat ggcggcggga 1021 aaagcgagcg gcgagagcga ggaggcgtcc cccagcctga cagccgagga gagggaggcg 1081 ctcggcggac tggacagccg cctctttggg ttcgtgagat ttcatgaaga tggcgccagg 1141 acgaaggccc tactgggcaa ggctgttcgc tgctatgaat ctctaatctt aaaagctgaa 1201 ggaaaagtgg agtctgattt cttttgtcaa ttaggtcact tcaacctctt attggaagat 1261 tatccaaaag cattatctgc ataccagagg tactacagtt tacagtctga ctactggaag 1321 aatgctgcct ttttatatgg tcttggtttg gtctacttcc attataatgc atttcagtgg 1381 gcaattaaag catttcagga ggtgctttat gttgatccca gcttttgtcg agccaaggaa 1441 attcatttac gacttgggct tatgttcaaa gtgaacacag actatgagtc tagtttaaag 1501 cattttcagt tagctttggt tgactgtaat ccctgcactt tgtccaatgc tgaaattcaa 1561 tttcacattg cccacttata tgaaacccag aggaaatatc attctgcaaa agaagcttat 1621 gaacaacttt tgcagacaga gaatctttct gcacaagtaa aagcaactgt cttacaacag 1681 ttaggttgga tgcatcacac tgtagatctc ctgggagata aagccaccaa ggaaagctat 1741 gctattcagt atctccaaaa gtccttggaa gcagatccta attctggcca gtcctggtat 1801 ttcctcggaa ggtgctattc aagtattggg aaagttcagg atgcctttat atcttacagg 1861 cagtctattg ataaatcaga agcaagtgca gatacatggt gttcaatagg tgtgctatat 1921 cagcagcaaa atcagcccat ggatgcttta caggcctata tttgtgctgt acaattggac 1981 catggccatg ctgcagcctg gatggaccta ggcactctct atgaatcctg caaccagcct 2041 caggatgcca ttaaatgcta cttaaatgca actagaagca aaagttgtag taatacctct 2101 gcacttgcag cacgaattaa gtatttacag gctcagttgt gtaaccttcc acaaggtagt 2161 ctacagaata aaactaaatt acttcctagt attgaggagg cgtggagcct accaattccc 2221 gcagagctta cctccaggca gggtgccatg aacacagcac agcagaatac ttctgacaat 2281 tggagtggtg gacatgctgt gtcacatcct ccagtacagc aacaagctca ttcatggtgt 2341 ttgacaccac agaaattaca gcatttggaa cagctccgcg caaatagaaa taatttaaat 2401 ccagcacaga aactgatgct ggaacagctg gaaagtcagt ttgtcttaat gcaacaacac 2461 caaatgagac caacaggagt tgcacaggta cgatctactg gaattcctaa tgggccaaca 2521 gctgactcat cactgcctac aaactcagtc tctggccagc agccacagct tgctctgacc 2581 agagtgccta gcgtctctca gcctggagtc cgtcctgcct gccctgggca gcctttggcc 2641 aatggaccct tttctgcagg ccatgttccc tgtagcacat caagaacgct gggaagtaca 2701 gacactattt tgataggcaa taatcatata acaggaagtg gaagtaatgg aaacgtgcct 2761 tacctgcagc gaaacgcact cactctacct cataaccgca caaacctgac cagcagcgca 2821 gaggagccgt ggaaaaacca actatctaac tccactcagg ggcttcacaa aggtcagagt 2881 tcacattcgg caggtcctaa tggtgaacga cctctctctt ccactgggcc ttcccagcat 2941 ctccaggcag ctggctctgg tattcagaat cagaacggac atcccaccct gcctagcaat 3001 tcagtaacac agggggctgc tctcaatcac ctctcctctc acactgctac ctcaggtgga 3061 caacaaggca ttaccttaac caaagagagc aagccttcag gaaacatatt gacggtgcct 3121 gaaacaagca ggcacactgg agagacacct aacagcactg ccagtgtcga gggacttcct 3181 aatcatgtcc atcagatgac ggcagatgct gtttgcagtc ctagccatgg agattctaag 3241 tcaccaggtt tactaagttc agacaatcct cagctctctg ccttgttgat gggaaaagcc 3301 aataacaatg tgggtactgg aacctgtgac aaagtcaata acatccaccc agctgttcat 3361 acaaagactg ataactctgt tgcctcttca ccatcttcag ccatttcaac agcaacacct 3421 tctccaaaat ccactgagca gacaaccaca aacagtgtta ccagccttaa cagccctcac 3481 agtgggctac acacaattaa tggagaaggg atggaagaat ctcagagccc catgaaaaca 3541 gatctgcttc tggttaacca caaacctagt ccacagatca taccatcaat gtctgtgtcc 3601 atatacccca gctcagcaga agttctgaag gcatgcagga atctaggtaa aaatggctta 3661 tctaacagta gcattttgtt ggataaatgt ccacctccaa gaccaccatc ttcaccatac 3721 cctcccttgc caaaggacaa gttgaatcca cctacaccta gtatttactt ggaaaataaa 3781 cgtgatgctt tctttcctcc attacatcaa ttttgtacaa atccgaacaa ccctgttaca 3841 gtaatacgtg gccttgctgg agctcttaag ttagacctgg gacttttctc tactaaaact 3901 ttggtggaag ctaacaatga acatatggta gaagtgagga cacagttgtt gcagccagca 3961 gatgaaaact gggatcccac tggaacaaag aaaatctggc attgtgaaag taatagatct 4021 catactacaa ttgctaaata tgcacagtac caggcctcct cattccagga atcattgaga 4081 gaagaaaatg aaaaaagaag tcatcataaa gaccactcag atagtgaatc tacatcgtca 4141 gataattctg ggaggaggag gaaaggaccc tttaaaacca taaagtttgg gaccaatatt 4201 gacctatctg atgacaaaaa gtggaagttg cagctacatg agctgactaa acttcctgct 4261 tttgtgcgtg tcgtatcagc aggaaatctt ctaagccatg ttggtcatac catattgggc 4321 atgaacacag ttcaactata catgaaagtt ccagggagca gaacaccagg tcatcaggaa 4381 aataacaact tctgttcagt taacataaat attggcccag gtgactgtga atggtttgtt 4441 gttcctgaag gttactgggg tgttctgaat gacttctgtg aaaaaaataa tttgaatttc 4501 ctaatgggtt cttggtggcc caatcttgaa gatctttatg aagcaaatgt tccagtgtat 4561 aggtttattc agcgacctgg agatttggtc tggataaatg caggcactgt tcattgggtt 4621 caggctattg gctggtgcaa caacattgct tggaatgttg gtccacttac agcctgccag 4681 tataaattgg cagtggaacg gtacgaatgg aacaaattgc aaagtgtgaa gtcaatagta 4741 cccatggttc atctttcctg gaatatggca cgaaatatca aggtctcaga tccaaagctt 4801 tttgaaatga ttaagtattg tcttctaaga actctgaagc aatgtcagac attgagggaa 4861 gctctcattg ctgcaggaaa agagattata tggcatgggc ggacaaaaga agaaccagct 4921 cattactgta gcatttgtga agtggaggtt tttgatctgc tttttgtcac taatgagagt 4981 aattcacgaa agacctacat agtacattgc caagattgtg cacgaaaaac aagcggaaac 5041 ttggaaaact ttgtggtgct agaacagtac aaaatggagg acctgatgca agtctatgac 5101 caatttacat tagctcctcc attaccatcc gcctcatctC TCGAGACCGG TGACTACAAA 5161 GACCATGACG GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA TGACAAGTGA 5221 CCGGTaAGTT TAAACCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT 5281 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC 5341 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT 5401 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT 5461 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC 5521 CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 5581 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 5641 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 5701 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 5761 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 5821 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 5881 TAAGGGATTT TGGCCATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 5941 AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC 6001 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT 6061 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 6121 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 6181 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG 6241 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC 6301 CgggATGGCC AAGCCTTTGT CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC 6361 AATCAACAGC ATCCCCATCT CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA 6421 CGGCCGCATC TTCACTGGTG TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT 6481 CGTGGTGCTG GGCACTGCTG CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT 6541 CGGAAATGAG AACAGGGGCA TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA 6601 TCTGCATCCT GGGATCAAAG CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG 6661 GATTCGTGAA TTGCTGCCCT CTGGTTATGT GTGGGAGGGC TAATTCGAAA TGACCGACCA 6721 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 6781 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 6841 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 6901 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 6961 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 7021 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 7081 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 7141 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 7201 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 7261 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 7321 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 7381 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 7441 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 7501 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 7561 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 7621 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 7681 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 7741 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 7801 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 7861 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 7921 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT 7981 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 8041 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 8101 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 8161 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 8221 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 8281 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 8341 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 8401 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 8461 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 8521 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 8581 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 8641 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 8701 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 8761 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 8821 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 8881 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 8941 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 9001 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 9061 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 9121 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 9181 TGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.