pCMV-LPIN1-3×FLAG-Neo plasmid

PVT107321

pCMV-LPIN1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-LPIN1(human)-3×FLAG-Neo
Alias: NM_145693.4;PAP1
Gene lenght: 2670
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-LPIN1-3×FLAG-Neo plasmid Description

pCMV-LPIN1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-LPIN1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgaa ttacgtgggg cagttagccg 781 gccaggtgtt tgtcaccgtg aaggagctct acaaggggct gaatcccgcc acactctcag 841 ggtgcattga catcattgtc atccgccagc ccaatggaaa cctccaatgc tcccctttcc 901 acgtccgctt tgggaagatg ggggtcctgc gctcccgaga gaaagtggtt gacatagaaa 961 tcaatgggga atctgtggat ttgcatatga aattgggaga taatggagaa gcattttttg 1021 ttcaagaaac agataatgat caggaagtta tccctatgca cctggccacc tcccccatcc 1081 tgtcagaagg agcttcgaga atggaatgcc agctgaaaag gggctctgtg gacaggatga 1141 gaggcctgga ccccagcacg ccagcccaag tgatcgctcc cagcgagacg ccgtcaagca 1201 gctctgtagt aaagaagaga agaaaaagga ggagaaagtc acagctggac agcctgaaga 1261 gagatgacaa catgaacaca tctgaggatg aggacatgtt ccccatcgag atgagctcgg 1321 atgaggccat ggagctgctg gagagcagca gaactcttcc taatgatata cctccattcc 1381 aagatgatat tcctgaggaa aacctctccc tggctgtgat ttaccctcag tcagcctcat 1441 accctaattc ggatagagag tggtcaccca ctcccagtcc ttccggttcc cgaccttcaa 1501 cacctaaaag tgattcagaa ttggtcagca agtccacgga aaggacaggg cagaagaacc 1561 cagaaatgct ttggctgtgg ggagagctgc cgcaggctgc taagtcttct tctccacaca 1621 agatgaaaga gtccagccca ttgagcagta gaaaaatttg tgataaaagt cactttcagg 1681 ccattcacag cgaatcttca gacactttta gtgaccaatc gccaactctg gtcggtgggg 1741 cacttttgga ccagaacaag cctcagacag aaatgcagtt tgtgaatgaa gaagacctgg 1801 agaccttagg agcagcagcg ccactcttgc ccatgatcga ggagctcaaa cccccctctg 1861 ccagtgtagt ccagacagca aacaagacgg attctccttc caggaaaaga gataaacgaa 1921 gccgacatct tggtgctgac ggcgtctact tggatgacct cacagacatg gatcctgaag 1981 tggcggccct gtattttccc aaaaacggag atccttccgg actcgcaaaa catgcaagcg 2041 acaacggagc ccggtcagcc aaccagtccc cgcagtcggt gggcagctcg ggcgtggaca 2101 gtggcgtgga gagcacctcg gacgggctga gggacctccc ttccatcgcc atctccctct 2161 gcgggggcct cagcgaccac cgggagatca cgaaagatgc attcctggag caagctgtgt 2221 catatcaaca gtttgtggac aaccccgcta ttatcgatga ccccaatctc gtggtaaaga 2281 ttgggagtaa atattataac tggacaacag cagcacccct cctcctggca atgcaggcct 2341 tccagaaacc tttgccaaag gccactgtgg aatctatcat gagggataaa atgcccaaaa 2401 agggaggaag atggtggttt tcatggaggg gaagaaacac cacaatcaag gaggaaagta 2461 agccagagca gtgcttggct ggcaaggccc atagcaccgg agagcaaccg ccgcagctca 2521 gcttggccac cagggtaaag catgaatcat cctccagtga tgaggagcgc gcagctgcca 2581 agccatcaaa cgcaggccac ctccctcttc tgcctaatgt cagctacaag aagactctcc 2641 ggctgacttc cgagcagctt aaaagcttga agttgaagaa tggccccaac gacgtggttt 2701 tcagtgtcac cacgcagtac caaggcacgt gccgctgtga gggcaccatc tatctgtgga 2761 actgggatga taaagtcatc atttctgata ttgatgggac aattaccaga tcagatactc 2821 ttggccacat tttgcccacc cttgggaagg attggaccca tcagggcatc gctaagctgt 2881 accataaagt gagccagaat ggatataaat ttctctactg ttctgcccgt gccatcggga 2941 tggcggacat gacgcggggc tacctgcact gggtcaacga gaggggcacg gtgctgcccc 3001 aggggcccct gctgctgagt cccagcagcc tcttctctgc cctgcacaga gaagtgattg 3061 aaaagaagcc agaaaagttt aaagtccagt gtttgacaga catcaaaaac ctgtttttcc 3121 ccaacacaga acccttttat gctgcttttg gaaaccgacc agctgatgtg tattcataca 3181 agcaagtagg agtgtctttg aatagaatat ttaccgtcaa ccctaaagga gagctggtac 3241 aggaacatgc aaagaccaac atctcttcgt atgtgagact ctgtgaagta gtcgaccacg 3301 ttttcccgtt gctgaaaaga agccattctt cagactttcc ctgttcggat accttcagta 3361 acttcacctt ttggagagag ccactgccac cttttgaaaa ccaggacatt cattctgcct 3421 cagcgGGATC CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA 3481 AGGATGACGA TGACAAGTAG TGAGGGCCCG ATATCTCGAC AATCAACCTC TGGATTACAA 3541 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 3601 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 3661 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 3721 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 3781 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 3841 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 3901 GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT 3961 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 4021 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG 4081 TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAAACGGG GGAGGCTAAC TGAAACACGG 4141 AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG 4201 CACGGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT 4261 CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC 4321 CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC 4381 TGCCATAGCA GATCTGCGCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG 4441 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC 4501 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC 4561 CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 4621 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC 4681 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC 4741 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT 4801 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG 4861 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 4921 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 4981 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 5041 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 5101 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG 5161 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 5221 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 5281 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 5341 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 5401 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 5461 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 5521 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 5581 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 5641 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 5701 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 5761 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 5821 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 5881 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 5941 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 6001 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 6061 GGACTCTGGG GTTCGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT 6121 CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG 6181 CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT 6241 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 6301 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 6361 CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT 6421 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 6481 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 6541 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 6601 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 6661 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 6721 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 6781 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 6841 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 6901 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 6961 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT 7021 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 7081 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 7141 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 7201 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 7261 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 7321 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 7381 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 7441 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 7501 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 7561 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 7621 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 7681 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 7741 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 7801 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 7861 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 7921 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 7981 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 8041 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 8101 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 8161 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 8221 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 8281 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 8341 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 8401 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 8461 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 8521 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC 8581 GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT 8641 CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA 8701 ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.