pCMV-lukG-3×FLAG-P2A-lukH(Staphylococcus)-6×His-Neo plasmid

PVT91218

pCMV-lukG-3×FLAG-P2A-lukH(Staphylococcus)-6×His-Neo plasmid Information

Plasmid name: pCMV-lukG(Staphylococcus)-3×FLAG-P2A-lukH(Staphylococcus)-6×His-Neo
Alias: -
Gene lenght: 1014bp+1053bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Staphylococcus
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-lukG-3×FLAG-P2A-lukH(Staphylococcus)-6×His-Neo plasmid Description

pCMV-lukG-3×FLAG-P2A-lukH(Staphylococcus)-6×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-lukG-3×FLAG-P2A-lukH(Staphylococcus)-6×His-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCATG ATTAAACAAC TATGTAAAAA TATCACAATT TGTACGTTAG CACTATCGAC 961 TACTTTCACT GTATTACCAG CTACTTCATT TGCAAAGATT AATTCTGAAA TCAAACAAGT 1021 TTCTGAGAAG AATCTTGATG GTGATACTAA AATGTATACA CGTACAGCTA CAACAAGTGA 1081 TAGTCAAAAA AATATTACTC AAAGCTTACA ATTTAATTTC TTAACTGAAC CTAATTATGA 1141 TAAAGAAACA GTATTTATTA AAGCAAAAGG TACAATTGGT AGTGGTTTGA GAATTTTAGA 1201 CCCAAATGGT TATTGGAATA GTACATTAAG ATGGCCTGGA TCTTATTCAG TTTCAATTCA 1261 AAATGTTGAT GACAACAACA ATACAAATGT GACTGACTTT GCACCAAAAA ATCAGGATGA 1321 ATCAAGAGAA GTTAAATATA CGTATGGTTA TAAAACAGGT GGAGATTTTT CGATTAATCG 1381 TGGAGGCTTA ACTGGAAATA TTACAAAAGA GAGTAATTAT TCAGAGACGA TTAGTTATCA 1441 ACAACCATCA TATCGTACAT TACTTGATCA ATCTACGTCA CATAAAGGTG TAGGTTGGAA 1501 AGTAGAAGCA CATTTGATAA ATAATATGGG ACATGACCAT ACGAGACAAT TAACTAATGA 1561 TAGTGATAAT AGAACTAAAA GTGAAATTTT TTCTTTAACA CGAAATGGAA ATTTATGGGC 1621 GAAAGATAAT TTCACACCTA AAGACAAAAT GCCTGTAACT GTGTCTGAAG GGTTTAATCC 1681 AGAATTTTTA GCTGTTATGT CACATGATAA AAAAGACAAA GGTAAATCAC AATTTGTTGT 1741 TCATTATAAA AGATCAATGG ATGAGTTTAA AATAGATTGG AATCGCCATG GTTTCTGGGG 1801 CTATTGGTCT GGTGAAAACC ATGTAGATAA AAAAGAAGAA AAATTATCAG CATTATATGA 1861 AGTTGATTGG AAGACACATA ATGTGAAGTT TGTAAAAGTA CTTAATGATA ATGAAAAGAA 1921 ACTCGAGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 1981 TGACGATGAC AAGGCCACTA ACTTCTCCCT GTTGAAACAA GCAGGGGATG TCGAAGAGAA 2041 TCCCGGGCCA ATGAAAAATA AAAAACGTGT TTTAATAGCG TCATCATTAT CATGTGCAAT 2101 TTTATTGTTA TCAGCAGCAA CGACTCAAGC AAATTCAGCT CATAAAGACT CTCAAGACCA 2161 AAATAAGAAA GAACATGTTG ATAAGTCTCA ACAAAAAGAC AAACGTAATG TTACTAATAA 2221 AGATAAAAAT TCAACAGCAC CGGATGATAT TGGGAAAAAC GGTAAAATCA CAAAACGAAC 2281 TGAAACAGTA TATGATGAGA AAACAAATAT ACTCCAAAAT TTACAATTCG ACTTTATCGA 2341 TGATCCAACT TATGACAAGA ATGTATTACT TGTTAAAAAA CAAGGCTCAA TTCATTCAAA 2401 TTTAAAGTTT GAATCTCATA AAGAAGAAAA AAATTCAAAT TGGTTAAAGT ATCCAAGTGA 2461 GTACCATGTA GATTTTCAAG TAAAAAGAAA TCGTAAAACT GAAATATTAG ACCAATTGCC 2521 GAAAAATAAA ATTTCAACTG CAAAAGTAGA CAGTACATTT TCATATAGCT CAGGTGGTAA 2581 ATTCGATTCA ACAAAAGGTA TTGGACGAAC TTCATCAAAT AGCTACTCCA AAACGATTAG 2641 TTATAATCAG CAAAATTATG ACACAATTGC CAGCGGTAAA AATAATAACT GGCATGTACA 2701 CTGGTCAGTT ATTGCGAATG ACTTGAAGTA TGGTGGAGAA GTGAAAAATA GAAATGATGA 2761 ATTATTATTC TATAGAAATA CGAGAATTGC TACTGTAGAA AACCCTGAAC TAAGCTTTGC 2821 TTCAAAATAT AGATACCCAG CATTAGTAAG AAGTGGCTTT AATCCAGAAT TTTTAACTTA 2881 TTTATCTAAT GAAAAGTCAA ATGAGAAAAC GCAATTTGAA GTAACATACA CACGAAATCA 2941 AGATATTTTG AAAAACAGAC CTGGAATACA TTATGCACCT CCAATTTTAG AAAAAAATAA 3001 AGATGGTCAA AGATTAATTG TCACTTATGA AGTTGATTGG AAAAATAAAA CAGTTAAAGT 3061 CGTTGATAAA TATTCTGATG ACAATAAACC TTATAAAGAA GGAGCGGCCG CACATCACCA 3121 TCATCACCAT TGACTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT 3181 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 3241 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 3301 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 3361 AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC 3421 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 3481 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 3541 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 3601 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 3661 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 3721 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 3781 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 3841 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA 3901 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 3961 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 4021 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 4081 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 4141 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 4201 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 4261 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 4321 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 4381 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 4441 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 4501 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 4561 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 4621 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 4681 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 4741 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 4801 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 4861 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 4921 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 4981 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 5041 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 5101 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 5161 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 5221 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 5281 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 5341 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 5401 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 5461 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 5521 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 5581 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 5641 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 5701 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 5761 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 5821 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 5881 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 5941 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 6001 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 6061 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 6121 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 6181 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 6241 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 6301 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 6361 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 6421 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 6481 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 6541 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 6601 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 6661 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 6721 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 6781 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 6841 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 6901 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 6961 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 7021 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 7081 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 7141 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 7201 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 7261 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 7321 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 7381 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 7441 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 7501 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 7561 AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???