pCMV-mCherry-CAPRIN1-6×HA-Puro plasmid

PVT93367

pCMV-mCherry-CAPRIN1-6×HA-Puro plasmid Information

Plasmid name: pCMV-mCherry-CAPRIN1(human-toxic-opt)-6×HA-Puro
Alias: NM_005898.5;CONDCAC; GPIAP1; GPIP137; GRIP137; M11S1;NEDLAAD; p137GPI; RNG105
Gene lenght: 2127bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pCMV-mCherry-CAPRIN1-6×HA-Puro plasmid Description

pCMV-mCherry-CAPRIN1-6×HA-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-mCherry-CAPRIN1-6×HA-Puro plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCCAC Catggtgagc aagggcgagg aggataacat 901 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 961 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 1021 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 1081 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 1141 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 1201 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg 1261 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 1321 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 1381 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 1441 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 1501 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 1561 catggacgag ctgtacaagg GaGGTGGAGG TAGTGGAGGA GGCGGAtcAA GCTTCATGCC 1621 CTCGGCCACC AGCCACAGCG GGAGCGGCAG CAAGTCGTCC GGACCGCCAC CGCCGTCGGG 1681 TTCCTCCGGG AGTGAGGCGG CCGCGGGAGC CGGGGCCGCC GCGCCGGCTT CTCAGCACCC 1741 CGCAACCGGC ACCGGCGCTG TCCAGACCGA GGCCATGAAG CAGATTCTCG GGGTGATCGA 1801 CAAGAAACTT CGGAACCTGG AGAAGAAAAA GGGTAAGCTT GATGATTACC AGGAACGAAT 1861 GAACAAAGGG GAAAGGCTTA ATCAAGATCA GCTGGATGCC GTTTCTAAGT ACCAGGAAGT 1921 CACAAATAAT TTGGAGTTTG CAAAAGAATT ACAGAGGAGT TTCATGGCAC TAAGTCAAGA 1981 TATTCAGAAA ACAATAAAGA AGACAGCACG TCGGGAGCAG CTTATGAGAG AAGAAGCTGA 2041 ACAGAAACGT TTAAAAACTG TACTTGAGCT ACAGTATGTT TTGGACAAAT TGGGAGATGA 2101 TGAAGTGCGG ACTGACCTGA AACAAGGTTT GAATGGAGTG CCAATATTGT CCGAAGAGGA 2161 GTTGTCATTG TTGGATGAAT TCTATAAGCT AGTAGACCCT GAACGGGACA TGAGCTTGAG 2221 GTTGAATGAA CAGTATGAAC ATGCCTCCAT TCACCTGTGG GACCTGCTGG AAGGGAAGGA 2281 AAAACCTGTA TGTGGAACCA CCTATAAAGT TCTAAAGGAA ATTGTTGAGC GTGTTTTTCA 2341 GTCAAACTAC TTTGACAGCA CCCACAACCA CCAGAATGGG CTGTGTGAGG AAGAAGAGGC 2401 AGCCTCAGCA CCTGCAGTTG AAGACCAGGT ACCTGAAGCT GAACCTGAGC CAGCAGAAGA 2461 GTACACTGAG CAAAGTGAAG TTGAATCAAC AGAGTATGTA AATAGACAGT TCATGGCAGA 2521 AACACAGTTC ACCAGTGGTG AAAAGGAGCA GGTAGATGAG TGGACAGTTG AAACGGTTGA 2581 GGTGGTAAAT TCACTCCAGC AGCAACCTCA GGCTGCATCC CCTTCAGTAC CAGAGCCCCA 2641 CTCTTTGACT CCAGTGGCTC AGGCAGATCC CCTTGTGAGA AGACAGCGAG TACAAGACCT 2701 TATGGCCCAG ATGCAGGGCC CCTACAATTT CATCCAGGAC AGCATGCTGG ACTTCGAAAA 2761 CCAGACACTG GACCCCGCCA TCGTTAGCGC CCAGCCAATG AATCCCACAC AGAATATGGA 2821 TATGCCCCAG CTGGTGTGCC CACCCGTGCA CTCTGAAAGC CGCCTGGCCC AGCCCAACCA 2881 GGTGCCCGTG CAGCCAGAGG CCACACAGGT GCCTCTGGTG AGCAGCACCT CTGAGGGCTA 2941 CACTGCCTCC CAGCCCCTGT ACCAGCCCTC CCACGCCACC GAGCAGCGAC CTCAGAAAGA 3001 GCCTATCGAC CAGATTCAGG CCACTATCTC TCTGAATACA GACCAGACTA CCGCCTCCTC 3061 CAGCCTGCCC GCCGCCAGCC AACCCCAGGT GTTCCAGGCC GGCACAAGTA AACCCCTGCA 3121 CAGTTCAGGA ATCAATGTGA ACGCCGCCCC TTTTCAGTCC ATGCAGACCG TGTTCAACAT 3181 GAACGCTCCT GTGCCCCCAG TGAATGAGCC CGAAACTCTG AAACAGCAGA ACCAGTACCA 3241 GGCTAGCTAT AACCAGTCCT TTTCATCCCA GCCTCACCAG GTGGAACAGA CAGAGCTGCA 3301 GCAGGAGCAG CTGCAGACTG TGGTGGGAAC CTACCACGGG TCTCCAGACC AAAGCCACCA 3361 GGTGACCGGC AACCACCAGC AGCCCCCTCA GCAGAATACC GGATTTCCAA GGAGCAACCA 3421 GCCTTACTAT AATTCCAGGG GCGTGAGCAG AGGCGGAAGC CGCGGCGCCA GAGGGCTGAT 3481 GAACGGATAC CGCGGACCCG CCAACGGGTT TAGAGGCGGG TATGACGGGT ACCGCCCATC 3541 CTTCTCCAAT ACACCAAACA GTGGATATAC CCAGTCCCAG TTTTCCGCCC CAAGGGATTA 3601 CTCAGGCTAC CAGAGGGACG GTTATCAGCA GAACTTCAAG AGGGGGAGCG GCCAGTCAGG 3661 ACCCAGAGGC GCCCCCAGAG GCAGGGGCGG CCCCCCTAGA CCCAACCGCG GCATGCCACA 3721 GATGAACACA CAGCAGGTGA ATTATCCGTA TGACGTTCCG GATTACGCGA CCTATCCGTA 3781 CGACGTTCCG GATTACGCTT CCTACCCATA CGACGTACCG GATTACGCGC TCGAGTACCC 3841 ATACGACGTA CCTGATTATG CAACTTATCC TTATGATGTA CCTGATTATG CTAGCTATCC 3901 TTATGATGTC CCAGACTACG CATAGTGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC 3961 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT 4021 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT 4081 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG 4141 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG 4201 AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC 4261 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC 4321 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA 4381 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT 4441 TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT 4501 GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA 4561 CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT 4621 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG 4681 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 4741 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 4801 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 4861 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 4921 GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 4981 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 5041 CAAGAGACAG GATGAGGATC GTTTCGCatg accgagtaca agcccacggt gcgcctcgcc 5101 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5161 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5221 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5281 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5341 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5401 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5461 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5521 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5581 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5641 tgcatgaccc gcaagcccgg tgcctgaGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 5701 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 5761 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 5821 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 5881 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 5941 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 6001 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 6061 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 6121 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 6181 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 6241 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 6301 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 6361 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 6421 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 6481 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 6541 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 6601 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 6661 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 6721 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 6781 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 6841 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 6901 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 6961 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 7021 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 7081 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 7141 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 7201 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 7261 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 7321 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 7381 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 7441 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 7501 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 7561 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 7621 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 7681 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 7741 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 7801 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 7861 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 7921 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 7981 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 8041 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 8101 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 8161 ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???