pCMV-MLLT10-3×HA-Neo plasmid

PVT110734

pCMV-MLLT10-3×HA-Neo plasmid Information

Plasmid name: pCMV-MLLT10(human)-3×HA-Neo
Alias: NM_004641.4;AF10
Gene lenght: 3081
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-MLLT10-3×HA-Neo plasmid Description

pCMV-MLLT10-3×HA-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-MLLT10-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atggtctcta gcgaccggcc cgtgtcactg 961 gaggacgagg tctcccatag tatgaaggag atgattggag gctgttgcgt ttgctcagac 1021 gagagaggct gggccgagaa cccgctggtt tattgcgacg ggcacggctg cagcgtcgcg 1081 gtgcatcaag cttgctatgg cattgttcaa gtacccactg gaccgtggtt ttgcaggaaa 1141 tgtgaatctc aggagagagc agccagagtg agatgtgaac tttgtcccca taaggatgga 1201 gctttaaaaa gaacagataa tgggggttgg gcccatgtgg tttgtgccct gtatattcca 1261 gaggtacaat ttgccaatgt ttccacaatg gaaccaattg ttttacagtc tgttccgcat 1321 gatcgttata ataagacttg ctacatttgt gatgaacaag gaagagaaag caaagcagcc 1381 actggtgctt gcatgacatg taataaacat ggatgtcgac aggctttcca tgtaacatgc 1441 gctcagtttg ccggactgct ttgtgaagaa gaaggtaatg gtgccgataa tgtccaatac 1501 tgtggctact gtaaatacca ttttagtaag ctgaaaaaga gcaaacgggg atctaatagg 1561 tcatatgatc aaagtttaag tgattcttcc tctcactctc aggataaaca tcatgagaaa 1621 gagaaaaaaa aatataaaga gaaggacaaa cacaaacaga aacacaagaa gcagccagaa 1681 ccatcacctg cattggttcc atccttgact gttactacag aaaaaactta tacaagcact 1741 agcaacaact ctatatctgg atcattgaag cgcttggaag atactactgc acgatttaca 1801 aatgcaaatt tccaggaagt ctctgcacac acctctagtg gaaaagatgt ttcagagact 1861 agagggtcag agggcaaagg gaagaaatct tcagctcaca gctcaggtca aaggggaaga 1921 aagcctggtg gtggaagaaa tccaggaaca actgtgtcag cagctagccc ttttcctcaa 1981 ggcagttttt caggaactcc aggcagtgta aagtcatctt ctggaagttc agtgcagtct 2041 ccccaggatt tcctgagctt tacagactca gatctgcgta atgacagtta ctctcactcc 2101 caacagtcat cagcaaccaa agatgtacat aaaggagagt ctggaagcca ggaagggggg 2161 gtaaatagtt ttagtacctt aattggcctc ccttcaacct cagctgttac ttcacagcct 2221 aaaagctttg aaaattcacc tggagatttg ggtaattcca gccttcctac agcaggatat 2281 aagcgggctc aaacttctgg catagaagaa gaaactgtaa aggaaaagaa aaggaaagga 2341 aataaacaaa gtaagcatgg gcctggcaga cccaaaggaa acaaaaatca agagaatgtt 2401 tctcatctct cagtttcttc tgcttcacca acatcatctg tagcatcagc tgcaggaagc 2461 ataacaagct ctagtctgca gaaatctcct acattgctca ggaatggaag tttacagagc 2521 ctcagtgttg gctcatctcc agttggttca gaaatttcca tgcagtatcg gcatgatgga 2581 gcttgcccaa caactacgtt ctcagagttg ctgaatgcaa tacacaacga cagaggtgac 2641 agttctacac taacaaagca agaacttaaa ttcataggta tttataacag caatgatgta 2701 gcagtatcgt ttccaaatgt agtatctggc tcgggatcta gtactcctgt ctccagctct 2761 cacttacctc agcagtcttc tgggcatttg caacaagtag gagcgctctc tccctcagct 2821 gtgtcatctg cagcccctgc tgttgctaca actcaggcaa atactctatc tggatcttct 2881 ctcagtcagg caccatctca tatgtatggc aatagatcaa attcatcaat ggcagctctt 2941 atagctcagt ctgaaaacaa tcaaacagat caagatcttg gagacaatag ccgcaaccta 3001 gttggcagag gaagctcacc ccgaggaagt ctctcgccac gatcccctgt aagcagctta 3061 cagattcgct atgatcaacc aggcaacagc agtttggaaa atctgcctcc agtagcagcc 3121 agcatagaac agcttttgga gaggcagtgg agtgaaggac agcaattttt actagaacag 3181 ggtactccta gtgacatttt aggaatgctg aagtcattac accaacttca agttgaaaac 3241 cgaagattag aggaacaaat taaaaacttg actgccaaaa aggaacggct tcagttattg 3301 aatgcacagc tttcagtgcc ttttccaaca ataacagcaa atcctagtcc gtctcatcaa 3361 atacacacat tttcagcaca gactgctcct actactgatt ccttgaacag cagtaagagc 3421 cctcatatag gaaacagctt tttacctgat aattctcttc ctgtattaaa tcaggactta 3481 acctccagtg gacaaagtac cagcagctca tcagctcttt ctaccccacc tcctgctggg 3541 cagagtccgg ctcaacaagg ctcaggagtg agtggagttc agcaggtcaa tggcgtgaca 3601 gtgggggcac tagctagtgg aatgcagcct gtaacttcca ccattcctgc cgtgtctgca 3661 gtgggtggaa taattggagc tttgccaggt aaccaactgg caattaatgg cattgtagga 3721 gctttaaatg gggttatgca gactcctgtc acaatgtccc agaaccctac ccctctcacc 3781 cacacaaccg taccacctaa tgcaacacat ccaatgccag ctacactgac taacagtgcc 3841 tcaggactag gattactttc tgaccagcaa cgacaaatac ttattcatca acagcagttt 3901 cagcagttgt taaattctca acagctcaca ccagtacaca ggcaccccca cttcacacag 3961 ctaccaccaa cccatttctc accatccatg gagataatgc aagtcagaaa gCTCGAGTAC 4021 CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG TACCTGATTA TGCTAGCTAT 4081 CCTTATGATG TCCCAGACTA CGCAtgaGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT 4141 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG 4201 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG 4261 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG 4321 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA 4381 ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 4441 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 4501 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 4561 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 4621 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 4681 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 4741 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 4801 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT 4861 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 4921 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 4981 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 5041 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 5101 CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 5161 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC 5221 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC 5281 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT 5341 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 5401 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA 5461 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 5521 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA 5581 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC 5641 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 5701 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG 5761 CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 5821 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC 5881 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC 5941 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC 6001 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA 6061 AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT 6121 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG 6181 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG 6241 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 6301 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC 6361 TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 6421 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 6481 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 6541 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 6601 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 6661 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 6721 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 6781 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 6841 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 6901 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 6961 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 7021 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 7081 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 7141 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 7201 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 7261 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 7321 CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 7381 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 7441 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 7501 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 7561 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 7621 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 7681 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 7741 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 7801 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 7861 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 7921 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 7981 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 8041 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 8101 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 8161 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 8221 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 8281 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 8341 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 8401 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 8461 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 8521 AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.