pCMV-Nyap1-3×FLAG-Neo plasmid

PVT110446

pCMV-Nyap1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Nyap1(mouse)-3×FLAG-Neo
Alias: NM_175521.4;6430598A04Rik
Gene lenght: 2499
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Nyap1-3×FLAG-Neo plasmid Description

pCMV-Nyap1-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Nyap1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgaa ccttctctac cgaaaaacca 781 agctggagtg gagacagcac aaagaagagg aggccaaacg gagctccagt aaggaagcag 841 cccccacagg cccggtgggg cctggggctg tcccagggcc tggggttcga gtgcgagaca 901 ttgcctcgct tcgacgatcc ctcaggatgg gtttcatgac gatgcccgcg tcccaggaac 961 acacccctca cccctgccgc agcaccatgg ccccacgctc tctctcctgc cactcggtgg 1021 gcagcatgga cagtgtaggt ggtgggcctg ggggagggct cacggaggac agcagcaccc 1081 ggagaccccc agctaagccc cggagacacc ccagcaccaa gctcagcatg gcaggaccag 1141 gggcagagac acctccaagt aagaaagcag gctcccagaa gccagctccc gaatgccgtg 1201 agtccagccg gaaggttccc ccacagaaac ccaggcgaag ccccaatacc cagctgtctg 1261 tgtcctttga tgagtcttgt gccccagccc catctcctcg aggggcgaac ctgcccctgc 1321 aacgcctcag tagggcttct cgaataactg gagacctgga tgcaggggcc caggaagaag 1381 agccagtgta cattgagatg gtaggggatg tctttcgagg aggaggacga agtggaggag 1441 gcctggcggg acctcctctt ggaagtggag gcccaacccc tccagctgct gccgattcgg 1501 actctgaaga cagtgaggct atctatgaag agatgaagta ccccttgcct gaagaagcag 1561 gagatggcag ggccaacggg cctcctccat tgacggcacc ctcccctcca caacagaccc 1621 atatcctcca gcctcacccc cacccccatc gccgtccagc ttcagccctc ccaagccgga 1681 gggatgggac acccaccaag accacacctt gtgaaatacc gccacccttt cctaacctcc 1741 ttcagcaccg gcctccactt ctggccttcc ctcaagccaa gtctgcttca cgagcccctg 1801 gtgatggggt ctcaaggcta ccggtcctct gccactccaa ggagccagct ggttccaccc 1861 cagctcccca agtgcctgca cgagagcggg agacgcctcc cctaccacct ccgcctcctg 1921 ctgccaacct gctattgctg ggaccatcag gccgagcccg gagccactcg acaccgttgc 1981 caccccaggg ctctggccag accaggggag agcgggaact cccgaactct cacagcatga 2041 tctgccccaa ggcggcaggg gtacccgcag cccaccctgc cccagctgcc ttgctccccg 2101 gccctcccaa ggacaaggcc gtgtcgtaca ctatggtgta ttctgcggta aaagtgacca 2161 cacactcggt cctgccagct ggtccacccc tgggtgttgg agagccaaag acagaggaga 2221 tttcagtact tcatggaatg ctgtgtgcca gttcgaggcc ccctgtccca gggaaatcca 2281 gcccccatag cggggctatg ggttcagcag ctggggtcct ccaccaccgc agctgcctgg 2341 cctcccccca cagccttccg gatccaactg gaggttccct gactcccctc tggacctacc 2401 cagccacagc agctgggctc aagagacccc ctgcctatga cagcctcaag gctggggggg 2461 tgttgaataa gggctgtgga atgggggcac catcccccat ggtcaagatc cagctgcaag 2521 agcaagggac tgatgggggt gcctttgcca gcatctcctg tgcccatgtc attgccagtg 2581 cggggacgcc agaagaggaa gaagagatgg gggccgcgtt tggggcaggc tgggctctgc 2641 agaggaaggt cctgtatgga ggacggaagg caaaggaagt ggacacagaa gaggacggtg 2701 cccgagcctg gaatggcagt acagaaggtc caggcaaagt cgaacatgag gacaggggcc 2761 ctgtgccatc agggattcct gtgaggagcc agggggcaga gggtctgctg gccaggatcc 2821 accatgatcg aggagggagc cgcacagcgc tgcctgtccc ttgccagact ttccctgcct 2881 gccaccggaa tggagatttc acgggagggt accgcctggg ccgttctgcg tccacctctg 2941 gagtccgtca ggctgcactc cataccccac ggccctgcag ccagcccagg gatgccctga 3001 gccagacaca ccctgtgctg cccctgcccc tgccgcccca gccggctcgg gagcgcgatg 3061 gcaaactcct agaggtgatc gagcgcaaac gctgcgtgtg caaggagatc aaggcacgcc 3121 accgtcccga ccgggggctc tgcaagcaag agagcatgcc catcctcccc agctggcggc 3181 gggtccctga accccgcaag tctggtaccc cgccctgccg gcggcagcac acggtcctct 3241 gggacactgc catcGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA 3301 TCGACTACAA GGATGACGAT GACAAGTAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT 3361 GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT 3421 ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT 3481 TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT 3541 CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT 3601 TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC 3661 GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA 3721 CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC 3781 CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA 3841 CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC 3901 TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT 3961 GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG 4021 AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG 4081 GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT 4141 TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC 4201 GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC 4261 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT 4321 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC 4381 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT 4441 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC 4501 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT 4561 GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 4621 TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 4681 TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 4741 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 4801 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 4861 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 4921 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC 4981 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 5041 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 5101 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 5161 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 5221 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 5281 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 5341 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 5401 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 5461 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 5521 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 5581 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 5641 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 5701 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG 5761 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 5821 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 5881 TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC 5941 ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG 6001 GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC 6061 CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 6121 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 6181 TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT 6241 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 6301 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 6361 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 6421 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 6481 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 6541 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 6601 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 6661 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 6721 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 6781 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG 6841 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 6901 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 6961 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 7021 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 7081 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 7141 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 7201 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 7261 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 7321 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 7381 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 7441 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 7501 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 7561 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 7621 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 7681 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 7741 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 7801 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 7861 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 7921 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 7981 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 8041 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 8101 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 8161 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 8221 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 8281 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 8341 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG 8401 AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA 8461 AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT 8521 TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.