pCMV-Pde6c-3×FLAG-mCMV-EGFP plasmid

PVT98536

pCMV-Pde6c-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-Pde6c(mouse)-3×FLAG-mCMV-EGFP
Alias: NM_033614.3;cpfl1
Gene lenght: 2583bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Pde6c-3×FLAG-mCMV-EGFP plasmid Description

pCMV-Pde6c-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-Pde6c-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatgggt gagatcagcc aagaggccgt 661 ggagcgctac ctggagaaga acccttgttt tgccaaagag tattttgaca agaaactacg 721 ggtggaggca ctgggggtga tcttcaaaaa cagccatgca ggggtccaga ccggcttgtc 781 gctccctgag atgacacagg tggaggagtc ggccgtgtgc ctggagctac tgcagtgtat 841 gcaggacgag gccggaagcg cggagcagat ggctcaccgg gcactgcaga ggctcgctca 901 acttctgcag gctgattgct gcagtatgtt ctcgtgcaga gcaagaaacg gcataccaga 961 ggtggcctcc aggctgctga acgtcacccc aacttctaag tttgaggaca acctcgtggc 1021 ccctgacaga gaagttgtat ttccgttgga cattgggata gttggctggg tcgctcatgt 1081 gaagaaagcc cttaatgtct cagatgtcaa aaagaacagc catttttctg actttatgga 1141 caagcaaact ggatatgtca ccaggaatct gctagcagtc ccgatcgtgg caggcaagga 1201 ggtccttgct gtggtcatgg cagtgaacaa aatcagcgct cccgagtttt ccaaacagga 1261 tgaggaggtc ttttctaagt acctcagctt tgttgctgtc gcactaaggc tgcagcacac 1321 cagttacctg tacagtgtgg agtcccggag aagccagatc cttatgtggt cagccaataa 1381 agtattcgaa gagctcactg atgttgagcg acagtttcac aaggcactgt acaccatcag 1441 gacgtacctg aactgcgatc gctactccat tggtcttctg gacatgacca aggagaagga 1501 attttacgat gagtggccaa tcaagcttgg agaagttgag ccttataaag gaccaaagac 1561 tccagatggc agggaaatca tcttctataa aatcatcgat tacattttac atggaaaaga 1621 agagatcaac gtgattccgt cacctccagc agaccactgg acgcttgtta gtggattgcc 1681 aacatacgtt gcagaaaatg gatttatctg taacatgctg aatgcccctg cagatgagta 1741 cttcacattt cagaaaggac cggtagatga gactggctgg gtcattaaaa atgtcttgtc 1801 cctgcctatt gtcaacaaga aagaagacat cgtgggtgta gctacgtttt acaaccggaa 1861 ggatgggaaa ccctttgatg agcatgatga acatatcact gagactttaa cccagtttct 1921 tggatggtct cttttaaaca ccgataccta tgagcgagtg aacaagctgg aaagcagaaa 1981 ggacattgct caggaaatgg ttatgaacct cacaaaagcc accccggatg aaatcagctc 2041 tattctgaag tttaaagaga agttaaatgt agaggtaatt gaagagtgtg aagagagaca 2101 gctcctggcc atattgaagg aggacttgcc ggacccgaga acagcagacc tgtacgagtt 2161 ctgtttcagc gacttcccca ttacggagca cgagttggtt aaatgcgggc tgaggctgtt 2221 tttggagata aacgtggtgg agaaattcaa agtccctgta gaggttctta caaggtggat 2281 gtacacagtc cggaaagggt accgtcctgt cacctaccac aactggcggc acggattcaa 2341 cgtggggcag accatgttca ctttgttaat gacaggaaga ctaaagaaat actatacaga 2401 ccttgaagcc tttgccatgc tcgctgctgc tttctgccat gacattgacc acagaggcac 2461 caataacttg taccaaatga agtccacatc tccacttgca aggcttcatg ggacttccat 2521 tttagagcga catcacctgg aatatagcaa gactctcttg caggatgaga gtttaaacat 2581 cttccagaat ctaaacaagc ggcagtttga aacggtgatc catttgttcg aggttgcaat 2641 aatagcaact gacctggcac tctatttcaa gaagagaacc atgtttcaaa agatcgttga 2701 tacctgcgag cagatgcaaa gtgaagaaga aaccatcaaa tacgtaacct ccgacccgac 2761 caaaaaggaa gtcatcatgg ccatgatgat gactgcctgt gacctgtctg ccatcaccaa 2821 gccctgggag gtgcagagtc aggtagcact tctggttgca aatgaatttt gggaacaagg 2881 agatctggaa agaacagtgc tgcagcagca accaattcct atgatggaca gaagcaaaaa 2941 ggatgaactc ccgaaacttc aagtgggatt tattgatttt gtttgtactt tcgtatataa 3001 ggagttctct cggtttcatg gagagatcac acccatgctg aacggccttc agaataacag 3061 ggtggaatgg aaatccctgg cggaggagta cgaggcgaag gtgaaggtga ctgaagaaga 3121 ggcgggaaag caggaagaag aagcctcaga tggaaaagct gccacagatt taggaggcag 3181 cgccgaagac aaaaagtcca aaacatgctt aatgttaAAG CTTACGCGTG ACTACAAAGA 3241 CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTGAGG 3301 ATCCGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 3361 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 3421 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 3481 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 3541 GGATGCGGTG GGCTCTATGG TCTAGAGATA TACTGAGTCA TTAGGGACTT TCCAATGGGT 3601 TTTGCCCAGT ACATAAGGTC AATAGGGGTG AATCAACAGG AAAGTCCCAT TGGAGCCAAG 3661 TACACTGAGT CAATAGGGAC TTTCCATTGG GTTTTGCCCA GTACAAAAGG TCAATAGGGG 3721 GTGAGTCAAT GGGTTTTTCC CATTATTGGC ACGTACATAA GGTCAATAGG GGTGAGTCAT 3781 TGGGTTTTTC CAGCCAaTTT AATTAAAACG CCATGTACTT TCCCACCATT GACGTCAATG 3841 GGCTATTGAA ACTAATGCAA CGTGACCTTT AAACGGTACT TTCCCATAGC TGATTAATGG 3901 GAAAGTACCG TTCTCGAGCC AATACACGTC AATGGGAAGT GAAAGGGCAG CCAAAACGTA 3961 ACACCGCCCC GGTTTTCCCC TGGAAATTCC ATATTGGCAC GCATTCTATT GGCTGAGCTG 4021 CGTTCTACGT GGGTATAAGA GGCGCGACCA GCGTCGGTAC CGTCGCAGTC TTCGGTCTGA 4081 CCACCGTAGA ACGCAGATCa ccggtcgcca ccatggtgag caagggcgag gagctgttca 4141 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 4201 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 4261 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 4321 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 4381 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 4441 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 4501 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 4561 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 4621 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 4681 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 4741 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 4801 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag 4861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 4921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 4981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5041 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata 5101 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 5161 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 5221 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 5281 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 5341 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 5401 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 5461 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 5521 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 5581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 5641 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt 5701 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 5761 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 5821 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 5881 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 5941 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 6001 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc 6061 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 6121 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 6181 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 6241 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 6301 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 6361 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 6421 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 6481 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 6541 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 6601 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 6661 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 6721 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 6781 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 6841 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 6901 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 6961 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 7021 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 7081 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa 7141 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 7201 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 7261 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat 7321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 7381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 7441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 7501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 7561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 7621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 7681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 7741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 7801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 7861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 7921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 7981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 8041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 8101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 8161 catgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???