pCMV-Pdzrn4-3×FLAG-Neo plasmid

PVT111521

pCMV-Pdzrn4-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Pdzrn4(mouse)-3×FLAG-Neo
Alias: NM_001164593.1;1110017D07Rik; LNX4; mKIAA3027; SAMCAP3L
Gene lenght: 3042
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Pdzrn4-3×FLAG-Neo plasmid Description

pCMV-Pdzrn4-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Pdzrn4-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatggg cttcgctttg gagcgtctcg 781 cggaagccgt ggaccccgct ttccagtgcc agctactgtg tggccaggta ctggaggagc 841 ccgcgtgcac gccttgcggg cacgtcttct gcgccagctg cctgctgccc tgggcggcgg 901 ggagacgctg gtgcccgctg cagtgccagc cgctggcgcc gggcgagctg taccgggtgc 961 tgccgctgcg cagcctggtc cagcaactgc gcgtgcagtg caactacagc ggacagggct 1021 gtggccacag gggcgactgc gggtgctggc agtgcacatc cagcgctgcg ccttcggccc 1081 tgccagcggc tgtgacagcc actgcccagg cggctgcgga ggcggcgggg aggaaaagca 1141 cccggcgacc aagacctgct ggagtgggga cgcgcgtgag gggccacccg gacctggagg 1201 tcggcctctg gttgtcgcct gctgtcgggg tggggaagac gcggcgggcg caggtcagct 1261 tgttgcagcg caggtatcag gagaagttgg ctctgtacat ggcgcacacg cgcaacttcg 1321 tcgggaaccc gggaggccgc ggccaggatg gagagcagaa gccgttcact gttgtgttgg 1381 aaagagaaga tgacactttg ggattcaaca taataggagg ccgcccatat cagaattccc 1441 agaagcaatc agcgcctgaa ggaatttatg tctcaaaaat tttagaaaac ggacctgcag 1501 accgagcaga tggcctggag gttcatgaca aaatcattgc ggtcaatggc agggatctct 1561 caaaggccac tcatgaagag gcagtggaag cttttcgaac tgccaaggag cccatagtgg 1621 tacaggtgtt aaggcggact cccctcagcc ggccaacata cggcatgact ccggaagtac 1681 agctcacgaa tgccagcaca cagacggaca tcacttttga acacatcatg gccctggcga 1741 agcttcggcc actcacccct ccggtgcctg acacctgtcc atttttgctt tcagacagct 1801 gccactccct tcatccgatg gagcatgggt tttatgaaga ccatgagtac gtctccagct 1861 tgcccgctga tgcagacaga gcagatgact ttgaatatga ggaggtcgag ctgtgtcgtg 1921 ttagcagtca agagaagctg ggcctgacag tctgctaccg aaccgatgat gaagaagaca 1981 cgggcattta tgtcagcgag gttgacccaa atagcattgc tgccaaggat ggccggattc 2041 gagaagggga tcgtatttta caaataaatg gggaagacat ccaaaaccgg gaagaggccg 2101 tggctctgct ctccagcgat gagtgtaaga gaatcgtgct gctcatcgcc aggcccgaca 2161 tgcagctgga tgaaggctgg ctggaagatg aaaggaacga attcttagag gagttaaact 2221 cagagctgct ggaggaagac cataatgaag gagcacagcg cacagcctct gagctgcagc 2281 agccaaaaaa acaggaggaa gaagaaggca caacagacac ggccacctcc tcttccaaca 2341 accaggagaa ggacagtggc gtggggcgca cggatgagag cttgcggaat gatgagagct 2401 cagagcagga gaacgcagtg gaggagcccc acagcaccac cctgaagagc aagagagagc 2461 tggggaagag ccaagacacg ctagggagcc ttgagcatca atgcagtgag agttgtgctg 2521 ggggtgagtg cttagactcc gactgtgcca gcaaccagga ggtgtgtgaa ggcttccagc 2581 agctgttgga gcttaagatt cgaaaccacg gagattacga cctgtactac tcaagcagta 2641 ccatcgagtg caatcgaggg gaacaggatg gggtggagca cgagctgcag ctgcttaacg 2701 aggagctgcg aaacattgag ctagagtgcc agaacatcat gcaggcccac aggctccaga 2761 aagtgacgga ccaatacgga gacatctggg cattgcatga tgggggattc cgaaattaca 2821 acaccagcct ggatgtgcaa cgggggaaac tggatgacat catggaacac ccagaaaagt 2881 cagacaagga cagctcgagt gcgtacaata cggcagagag ctgcagaagc actccgctga 2941 ctgtagacag gtccccggac agttcccttc caagaatgat caacctcacc aataagaaaa 3001 atctgagaag catgatgaca gcccatcagt cccctcccag acagagcacc agggaataca 3061 catctaccaa agtcaaagcc accgatgaag gttgcagcgt cgaaagcctg gagaagggtc 3121 tagaaagcag ccagcttctg gaccaggagc acacagtcag tgagcatcct ccatacctct 3181 ctccttatca cagctcctcc tacagatatg ccaacattcc ggcgcatgcc cggcattatc 3241 aaagctacat gcagttaatt caacagaaat cggctgtgga gtatgcccag agtcagctca 3301 gcttggtgag catgtgcaag gaatcacaaa ggggctcgga gcccaagatg gaatggaagg 3361 taaaaattag gagtgatggg acccggtaca tcaccaagag gccagtgaga gaccgacttc 3421 tgaaggaacg tgccttaaag atcaaggagg agcggagtgg catgaccaca gacgacgaca 3481 ccatgagtga gatgaaaatg gggcgctact ggagcaagga ggagagaaag caacacctgg 3541 tccgggccaa ggagcaaagg cggcgccggg agttcatgat gcggagtcgg ctggagagcc 3601 tcaaagagag cccacagagc agcggcgaag gcaagaagga gctgagcatc atcgaactga 3661 gccacaaaaa gatgatgaaa aaaagaaaca agaaaatttt ggataactgg atgacaatcc 3721 aagaactgat gactcatggg gccaaatccc cagatggcac tagggtccat aatgcctttc 3781 tgtcggtgac caccgtaGGA TCCGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG 3841 ACATCGACTA CAAGGATGAC GATGACAAGT AGTGAGGGCC CGATATCTCG ACAATCAACC 3901 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC 3961 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT 4021 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT 4081 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG 4141 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC 4201 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC 4261 TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT 4321 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC 4381 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG 4441 CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAAACG GGGGAGGCTA 4501 ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA 4561 CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG 4621 CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC 4681 CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG 4741 GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC 4801 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 4861 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT 4921 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC 4981 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC 5041 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 5101 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 5161 GATTTTGGGG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 5221 GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 5281 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 5341 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 5401 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 5461 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA 5521 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 5581 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 5641 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 5701 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 5761 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 5821 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 5881 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 5941 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 6001 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 6061 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 6121 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 6181 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 6241 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG 6301 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 6361 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 6421 TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC 6481 ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT 6541 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA 6601 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 6661 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 6721 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA 6781 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 6841 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 6901 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 6961 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 7021 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 7081 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 7141 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 7201 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 7261 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 7321 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG 7381 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 7441 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 7501 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 7561 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 7621 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 7681 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 7741 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 7801 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 7861 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 7921 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 7981 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 8041 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 8101 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 8161 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 8221 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 8281 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 8341 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 8401 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 8461 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 8521 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 8581 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 8641 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 8701 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 8761 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 8821 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 8881 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC 8941 GGGAGATCTC CCGATCCCCT ATGGTCGACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG 9001 TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA 9061 ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.