pCMV-PGBD5-EGFP-Neo plasmid

PVT111202

pCMV-PGBD5-EGFP-Neo plasmid Information

Plasmid name: pCMV-PGBD5(human)-EGFP-Neo
Alias: NM_001258311.2
Gene lenght: 1572
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PGBD5-EGFP-Neo plasmid Description

pCMV-PGBD5-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-PGBD5-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc gccaccatgg ccgagggcgg cgggggcgcg cggaggaggg 961 cgccggcgct gctcgaggct gcccgcgcgc gctacgagag cctgcacatc tcggacgacg 1021 tgttcggcga gtccggcccg gacagcggcg ggaacccctt ctacagcacc tcggccgcct 1081 cgcgctcctc ctcggccgcc tcctcggacg acgagcgcga gcccccggga cccccagggg 1141 ccgccccgcc accgccccgc gccccggacg cacaggagcc ggaggaggac gaggccggcg 1201 cgggctggag cgcagcgctg cgggaccgcc cgcccccgcg cttcgaggat accggcggtc 1261 ccacccgaaa gatgcccccc agcgccagtg ccgtggactt cttccagctc tttgtcccag 1321 acaacgtcct caagaacatg gtggtgcaga caaacatgta tgccaagaag ttccaggagc 1381 ggtttgggag cgacggagcc tgggtggagg tgacgctgac ggagatgaag gcgttcctgg 1441 gctacatgat ctccaccagc atctcccact gcgagtccgt cctcagcatc tggagcggag 1501 gcttctacag caaccgcagc ctcgccctcg tcatgagcca ggcccgcttc gagaagatcc 1561 tcaagtactt ccacgtcgtg gccttccgct ccagccagac cacgcacggg ctctacaagg 1621 tccagccctt cctcgactcc ctgcagaaca gcttcgactc tgccttcagg ccttcccaaa 1681 cccaggtgct acatgaaccc ctgatcgatg aggatcctgt attcattgcc acgtgcacag 1741 agcgggagct gcgaaagagg aaaaagcgga aattcagcct ctgggtcaga caatgttctt 1801 ccactggctt catcatccag atttatgtcc acctgaagga aggtgggggc ccagatggcc 1861 tggatgcgct gaagaataag ccccagctcc acagcatggt ggccaggagc ctgtgccgga 1921 acgcggcagg caagaactac atcattttca cggggcccag catcaccagc ctgacgctgt 1981 ttgaagagtt tgagaagcaa gggatttact gctgcggctt gctccgcgcg cggaagagtg 2041 actgcaccgg cctcccactg tccatgctga ccaacccagc cacacccccg gcccggggcc 2101 agtaccaaat caagatgaag gggaacatgt ccttgatctg ctggtacaac aaaggacact 2161 tccgcttcct gaccaacgcc tactccccgg tgcagcaggg agtcatcatc aaaaggaaga 2221 gtggggagat cccatgcccc ttggccgtgg aggcgtttgc cgctcacctg agctacatct 2281 gcagatacga tgacaaatac agcaagtatt tcatttctca taaaccaaac aagacctggc 2341 agcaggtgtt ctggttcgcc atcagcatcg ccatcaacaa tgcctacatc ctgtacaaaa 2401 tgtcagacgc ctaccacgtg aagaggtaca gccgggcgca gtttggagag agactcgtca 2461 gagagctgct gggcttggag gatgcctctc cgacccacct cgagggcggt ggaggaagcg 2521 gcggaggtgg atctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 2581 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 2641 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 2701 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 2761 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 2821 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 2881 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 2941 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 3001 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 3061 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 3121 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 3181 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 3241 agctgtacaa gtagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt 3301 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 3361 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 3421 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 3481 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 3541 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 3601 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 3661 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 3721 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 3781 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 3841 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 3901 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 3961 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 4021 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 4081 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 4141 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 4201 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 4261 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 4321 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 4381 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 4441 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4501 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4561 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 4621 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4681 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4741 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4801 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4861 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 4921 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 4981 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5041 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5101 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5161 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 5221 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 5281 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 5341 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 5401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 5461 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 5521 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 5581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 5641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 5701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 5761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 6421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt 6481 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6541 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 6601 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 6661 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 6721 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 6781 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 6841 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 6901 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 6961 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 7021 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 7081 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 7141 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 7201 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 7261 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 7321 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 7381 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 7441 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 7501 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 7561 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 7621 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 7681 ccacctgacg tc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.