pCMV-Ppara-Neo plasmid

PVT111947

pCMV-Ppara-Neo plasmid Information

Plasmid name: pCMV-Ppara(mouse)-Neo
Alias: NM_011144.6;4933429D07Rik; Nr1c1; Ppar; PPAR-alpha; PPARalpha
Gene lenght: 1407
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ppara-Neo plasmid Description

pCMV-Ppara-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Ppara-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgaggcca ccatggtgga cacagagagc cccatctgtc 961 ctctctcccc actggaggca gatgacctgg aaagtccctt atctgaagaa ttcttacaag 1021 aaatgggaaa cattcaagag atttctcagt ccatcggtga ggagagctct ggaagctttg 1081 gttttgcaga ctaccagtac ttaggaagct gtccgggctc cgagggctct gtcatcacag 1141 acaccctctc tccagcttcc agcccttcct cagtcagctg ccccgtgatc cccgccagca 1201 cggacgagtc ccccggcagt gccctgaaca tcgagtgtcg aatatgtggg gacaaggcct 1261 cagggtacca ctacggagtt cacgcatgtg aaggctgtaa gggcttcttt cggcgaacta 1321 ttcggctgaa gctggtgtac gacaagtgtg atcggagctg caagattcag aagaagaacc 1381 ggaacaaatg ccagtactgc cgttttcaca agtgcctgtc tgtcgggatg tcacacaatg 1441 caattcgctt tggaagaatg ccaagatctg aaaaagcaaa actgaaagca gaaattctta 1501 cctgtgaaca cgacctgaaa gattcggaaa ctgcagacct caaatctctg ggcaagagaa 1561 tccacgaagc ctacctgaag aacttcaaca tgaacaaggt caaggcccgg gtcatactcg 1621 cgggaaagac cagcaacaac ccgccttttg tcatacatga catggagacc ttgtgtatgg 1681 ccgagaagac gcttgtggcc aagatggtgg ccaacggcgt cgaagacaaa gaggcagagg 1741 tccgattctt ccactgctgc cagtgcatgt ccgtggagac cgtcacggag ctcacagaat 1801 ttgccaaggc tatcccaggc tttgcaaact tggacttgaa cgaccaagtc accttgctaa 1861 agtacggtgt gtatgaagcc atcttcacga tgctgtcctc cttgatgaac aaagacggga 1921 tgctgatcgc gtacggcaat ggctttatca cacgcgagtt ccttaagaac ctgaggaagc 1981 cgttctgtga catcatggaa cccaagtttg acttcgctat gaagttcaat gccttagaac 2041 tggatgacag tgacatttcc ctgtttgtgg ctgctataat ttgctgtgga gatcggcctg 2101 gccttctaaa cataggctac attgagaagt tgcaggaggg gattgtgcac gtgcttaagc 2161 tccacctgca gagcaaccat ccagatgaca ccttcctctt cccaaagctc cttcaaaaaa 2221 tggtggacct tcggcagctg gtcacggagc atgcgcagct cgtacaggtc atcaagaaga 2281 ccgagtccga cgcagcgctg cacccactgt tgcaagagat ctacagagac atgtactgag 2341 gatccgagct cggtaccaag cttaagttta aaccgctgat cagcctcgac tgtgccttct 2401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 2461 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 2521 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 2581 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 2641 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 2701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 2761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 2821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 2881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 2941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 3001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 3061 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 3121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3181 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 3301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 3361 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 3421 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag 3481 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 3541 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 3601 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 3661 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 3721 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 3781 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 3841 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 3901 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 3961 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 4021 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 4081 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 4141 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 4201 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 4261 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 4321 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 4381 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 4441 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata 4501 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 4561 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag 4621 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 4681 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 4741 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 4801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 4861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 4921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 4981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 5041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 5101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 5161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 5221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 5281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 5341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 5401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 5461 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 5521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt 5581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 6001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 6061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 6121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 6181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 6361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 6421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 6481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 6541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6781 acctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.