pCMV-PPM1D-EYFP-Neo plasmid

PVT109828

pCMV-PPM1D-EYFP-Neo plasmid Information

Plasmid name: pCMV-PPM1D(human)-EYFP-Neo
Alias: NM_003620.4;IDDGIP; JDVS; PP2C-DELTA; WIP1
Gene lenght: 2157
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pCMV-PPM1D-EYFP-Neo plasmid Description

pCMV-PPM1D-EYFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-PPM1D-EYFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC atggcggggc tgtactcgct 661 gggagtgagc gtcttctccg accagggcgg gaggaagtac atggaggacg ttactcaaat 721 cgttgtggag cccgaaccga cggctgaaga aaagccctcg ccgcggcggt cgctgtctca 781 gccgttgcct ccgcggccgt cgccggccgc ccttcccggc ggcgaagtct cggggaaagg 841 cccagcggtg gcagcccgag aggctcgcga ccctctcccg gacgccgggg cctcgccggc 901 acctagccgc tgctgccgcc gccgttcctc cgtggccttt ttcgccgtgt gcgacgggca 961 cggcgggcgg gaggcggcac agtttgcccg ggagcacttg tggggtttca tcaagaagca 1021 gaagggtttc acctcgtccg agccggctaa ggtttgcgct gccatccgca aaggctttct 1081 cgcttgtcac cttgccatgt ggaagaaact ggcggaatgg ccaaagacta tgacgggtct 1141 tcctagcaca tcagggacaa ctgccagtgt ggtcatcatt cggggcatga agatgtatgt 1201 agctcacgta ggtgactcag gggtggttct tggaattcag gatgacccga aggatgactt 1261 tgtcagagct gtggaggtga cacaggacca taagccagaa cttcccaagg aaagagaacg 1321 aatcgaagga cttggtggga gtgtaatgaa caagtctggg gtgaatcgtg tagtttggaa 1381 acgacctcga ctcactcaca atggacctgt tagaaggagc acagttattg accagattcc 1441 ttttctggca gtagcaagag cacttggtga tttgtggagc tatgatttct tcagtggtga 1501 atttgtggtg tcacctgaac cagacacaag tgtccacact cttgaccctc agaagcacaa 1561 gtatattata ttggggagtg atggactttg gaatatgatt ccaccacaag atgccatctc 1621 aatgtgccag gaccaagagg agaaaaaata cctgatgggt gagcatggac aatcttgtgc 1681 caaaatgctt gtgaatcgag cattgggccg ctggaggcag cgtatgctcc gagcagataa 1741 cactagtgcc atagtaatct gcatctctcc agaagtggac aatcagggaa actttaccaa 1801 tgaagatgag ttatacctga acctgactga cagcccttcc tataatagtc aagaaacctg 1861 tgtgatgact ccttccccat gttctacacc accagtcaag tcactggagg aggatccatg 1921 gccaagggtg aattctaagg accatatacc tgccctggtt cgtagcaatg ccttctcaga 1981 gaatttttta gaggtttcag ctgagatagc tcgagagaat gtccaaggtg tagtcatacc 2041 ctcaaaagat ccagaaccac ttgaagaaaa ttgcgctaaa gccctgactt taaggataca 2101 tgattctttg aataatagcc ttccaattgg ccttgtgcct actaattcaa caaacactgt 2161 catggaccaa aaaaatttga agatgtcaac tcctggccaa atgaaagccc aagaaattga 2221 aagaacccct ccaacaaact ttaaaaggac attagaagag tccaattctg gccccctgat 2281 gaagaagcat agacgaaatg gcttaagtcg aagtagtggt gctcagcctg caagtctccc 2341 cacaacctca cagcgaaaga actctgttaa actcaccatg cgacgcagac ttaggggcca 2401 gaagaaaatt ggaaatcctt tacttcatca acacaggaaa actgtttgtg tttgcGAATT 2461 Cggtggaggc ggatcaggag gcggtggatc tCCggatcca ccggtcgcca ccatggtgag 2521 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 2581 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 2641 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 2701 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga 2761 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 2821 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 2881 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 2941 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 3001 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 3061 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 3121 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 3181 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg 3241 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 3301 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 3361 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 3421 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc 3481 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 3541 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 3601 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 3661 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 3721 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 3781 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 3841 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 3901 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 3961 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 4021 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga 4081 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 4141 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 4201 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4261 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4321 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 4381 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga 4441 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 4501 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 4561 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 4621 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 4681 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 4741 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 4801 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 4861 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 4921 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 4981 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 5041 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 5101 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 5161 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 5221 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 5281 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 5341 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 5401 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag 5461 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt 5521 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc 5581 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc 5641 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata 5701 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 5761 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 5821 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 5881 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 5941 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 6001 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 6061 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 6121 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 6181 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 6241 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 6301 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 6361 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 6421 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 6481 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 6541 gtggataacc gtattaccgc catgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.