pCMV-PSMA4-3×FLAG-IRES2-EGFP plasmid

PVT105651

pCMV-PSMA4-3×FLAG-IRES2-EGFP plasmid Information

Plasmid name: pCMV-PSMA4(human)-3×FLAG-IRES2-EGFP
Alias: NM_002789.6;HC9; HsT17706; PSC9;
Gene lenght: 783
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PSMA4-3×FLAG-IRES2-EGFP plasmid Description

pCMV-PSMA4-3×FLAG-IRES2-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-PSMA4-3×FLAG-IRES2-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagGC CACCatgtct cgaagatatg actccaggac cactatattt 661 tctccagaag gtcgcttata ccaagttgaa tatgccatgg aagctattgg acatgcaggc 721 acctgtttgg gaattttagc aaatgatggt gttttgcttg cagcagagag acgcaacatc 781 cacaagcttc ttgatgaagt ctttttttct gaaaaaattt ataaactcaa tgaggacatg 841 gcttgcagtg tggcaggcat aacttctgat gctaatgttc tgactaatga actaaggctc 901 attgctcaaa ggtatttatt acagtatcag gagccaatac cttgtgagca gttggttaca 961 gcgctgtgtg atatcaaaca agcttataca caatttggag gaaaacgtcc ctttggtgtt 1021 tcattgctgt acattggctg ggataagcac tatggctttc agctctatca gagtgaccct 1081 agtggaaatt acgggggatg gaaggccaca tgcattggaa ataatagcgc tgcagctgtg 1141 tcaatgttga aacaagacta taaagaagga gaaatgacct tgaagtcagc acttgcttta 1201 gctatcaaag tactaaataa gaccatggat gttagtaaac tctctgctga aaaagtggaa 1261 attgcaacac taacaagaga gaatggaaag acagtaatca gagttctcaa acaaaaagaa 1321 gtggagcagt tgatcaaaaa acatgaggaa gaagaagcca aagctgagcg tgagaagaaa 1381 gaaaaagaac agaaagaaaa ggataaaGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 1441 CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGgaat tctgcagtcg acggtaccgc 1501 gggcccggga tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg 1561 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 1621 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 1681 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 1741 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 1801 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 1861 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca 1921 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat 1981 ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc 2041 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac 2101 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 2161 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 2221 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 2281 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 2341 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 2401 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 2461 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 2521 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 2581 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 2641 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 2701 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 2761 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 2821 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 2881 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 2941 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3061 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 3121 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 3181 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 3241 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 3301 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 3361 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 3421 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 3481 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 3541 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 3601 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 3661 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 3961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 4021 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 4081 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 4141 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 4201 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 4261 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 4321 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 4381 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 4441 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 4501 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 4561 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 4621 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4681 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4741 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 4801 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 4861 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 4921 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 4981 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 5041 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 5101 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 5161 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 5221 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 5281 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 5341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5581 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 5641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 6001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 6121 cctgattctg tggataaccg tattaccgcc atgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???