pCMV-PTPN14-3×FLAG-Neo plasmid

PVT100416

pCMV-PTPN14-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-PTPN14(human)-3×FLAG-Neo
Alias: NM_005401.5;CATLPH; PEZ; PTP36; PTPD2
Gene lenght: 3561bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PTPN14-3×FLAG-Neo plasmid Description

pCMV-PTPN14-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PTPN14-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCatgcctt ttggtctgaa gctccgccgg acacggcgct acaacgtcct gagcaagaac 1021 tgctttgtca cacggattcg cctgctggac agcaatgtta tcgagtgcac gctgtcggtg 1081 gaaagcacag ggcaagaatg cctggaggct gtggcccaga ggctggagct gcgagagacg 1141 cactactttg gcctttggtt tctcagcaag agccagcaag cacgatgggt ggagctggag 1201 aaacctctga agaaacatct ggacaaattc gctaatgagc ctttgctttt ctttggagtc 1261 atgttctatg tgccaaatgt gtcatggctt cagcaagagg ccacaagata tcagtattac 1321 ctgcaagtca aaaaagatgt gcttgaaggg cgattacgat gtacattgga ccaggtgatt 1381 cggctagccg gcctagctgt gcaagctgat tttggagact ataatcagtt tgattctcaa 1441 gatttcctca gagagtatgt gctatttcct atggatttgg ccctggaaga ggctgttctg 1501 gaggagctga cccagaaggt agcccaagaa cacaaagccc acagtggaat cctgccagca 1561 gaagctgaac tgatgtacat caatgaagtt gaacgtttgg atggatttgg acaggaaatc 1621 ttccctgtaa aggacaatca tggaaactgt gtacaccttg gcattttctt tatggggatt 1681 ttcgtgagga acagaattgg aagacaagcg gtaatataca ggtggaatga catggggaat 1741 atcactcata acaagtcgac cattctagtg gagctcatca acaaagaaga gactgccctc 1801 tttcacacgg atgatatcga aaatgccaag tatatttctc ggttgtttgc cacacgacac 1861 aagttttaca aacaaaacaa aatctgcact gaacagtcaa attctccacc ccccatcaga 1921 cgccagccca cctggagccg atcctctctg cccaggcagc agccgtacat cctgcctccc 1981 gttcacgtcc agtgtggtga gcactactcg gaaacgcaca cctcgcaaga cagcattttt 2041 catgggaatg aagaagcctt gtattgcaac tctcacaaca gcctggactt aaattattta 2101 aatggcactg tcaccaatgg cagcgtgtgt agcgttcaca gcgtcaactc cctcaactgc 2161 tcgcaaagtt tcatccaggc ctcccctgta tcctccaacc tcagtatccc tgggagtgac 2221 atcatgcggg ccgactacat cccgagccac cggcacagcg cgatcatcgt gccctcgtac 2281 aggccaaccc ccgattatga gacagtcatg cgccagatga agagggggat cctgcataca 2341 gacagccaga gccagtctct gagaaacctc aacattatca acacccatgc ctacaaccag 2401 ccagaggatc tggtgtacag ccaaccggag atgcgggaga ggcaccccta cactgtccct 2461 tatgggccac agggggtcta cagcaacaaa cttgtcagtc catctgacca gaggaaccca 2521 aagaataatg tggtaccaag caagccgggg gcaagcgcca tctcgcacac ggtgagcacc 2581 ccagagctgg ccaacatgca gctgcagggc agccataact acagcacggc ccacatgctt 2641 aagaactatc tcttcaggcc accgcccccc tacccacggc cacgacctgc caccagcacc 2701 ccagacctgg ccagccaccg ccacaagtac gtcagcggca gcagcccgga cctggtgacc 2761 cggaaggtgc agctctcggt gaagaccttc caagaggaca gctctccggt ggttcatcag 2821 tctctccagg aggtgagcga gcccctcacg gccaccaagc accacggcac tgtgaacaag 2881 cgccacagcc tggaggtgat gaacagcatg gtgcggggca tggaggccat gacgctcaag 2941 tcgctccacc tccccatggc tcgccgcaac acgctccggg agcagggacc gcccgaggag 3001 gggtcaggca gccacgaggt cccccagctc cctcagtatc accacaagaa gaccttctct 3061 gatgccacta tgctaatcca cagcagcgag agtgaggagg aggaggagga ggctccagaa 3121 tcggtgcccc agatccccat gctccgggag aagatggagt acagtgccca gctgcaggcg 3181 gccctggccc gcatccccaa caagcccccg cctgagtacc ccggtccaag gaagagtgtg 3241 agcaatgggg ctctgaggca ggaccaagcc agccttcctc ccgccatggc cagagccagg 3301 gtgctgaggc atgggccagc caaggccatc agcatgtctc ggactgaccc gccggctgtc 3361 aacggggcct ctctcggccc atccatctcg gaacccgacc tgactagtgt gaaggagcgg 3421 gtcaaaaaag agcctgtgaa ggagagacct gtgtctgaaa tgttttccct ggaagacagc 3481 attatagaga gagagatgat gatcaggaat ctagagaagc agaagatggc aggcctggag 3541 gcacagaaga ggccgctgat gttggcagca ttgaatgggc tctcggtggc tcgagtctca 3601 gggcgggaag agaatcgagt tgatgccacc cgggttccca tggacgagag gttcagaacc 3661 ctgaagaaga aactagaaga gggaatggtg ttcacagaat atgagcaaat tccaaagaaa 3721 aaggcgaatg gcattttcag cacagcagct ctgccagaaa acgccgagcg cagccgaatc 3781 cgtgaagttg tcccctatga ggagaatcga gtagagctga taccaaccaa agaaaataac 3841 acaggataca ttaatgcctc ccacatcaag gtggtggttg gcggggcaga atggcactac 3901 atagccaccc aggggcccct gccacacacg tgccacgact tctggcagat ggtgtgggag 3961 cagggagtga atgtgattgc catggtcact gcagaggagg agggtggacg aaccaaaagc 4021 caccgatact ggcccaaact aggttcaaag cacagctcag ccacctatgg caagttcaag 4081 gtcaccacga agtttcgaac ggattctgtt tgctatgcaa ccacgggctt gaaggtcaag 4141 caccttttgt ctgggcaaga aaggacggtg tggcatttac aatatactga ctggccagat 4201 cacggctgtc cagaagatgt ccaaggattt ttatcctact tggaggagat ccagtcggtc 4261 cgtcgccata ccaacagcat gctggaaggc accaagaacc ggcacccgcc catcgtggtc 4321 cactgtagtg ctggggtggg aaggaccggc gtgctcattc tttctgagct gatgatctac 4381 tgcttggaac ataacgaaaa ggtggaagtg cccatgatgc tgaggctcct cagggagcag 4441 aggatgttca tgatccagac tatcgctcag tacaagtttg tctaccaagt cctcatccag 4501 ttcctccaaa actccagact cattCTCGAG GACTACAAAG ACCATGACGG TGATTATAAA 4561 GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT GAGGGCCCGT TTAAACCCGC 4621 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG 4681 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT 4741 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC 4801 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT 4861 TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC 4921 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 4981 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 5041 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 5101 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 5161 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 5221 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 5281 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT 5341 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 5401 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 5461 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 5521 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 5581 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT 5641 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA 5701 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT 5761 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 5821 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 5881 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 5941 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 6001 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 6061 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 6121 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 6181 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 6241 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 6301 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 6361 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 6421 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 6481 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG 6541 GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 6601 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 6661 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 6721 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 6781 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 6841 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 6901 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 6961 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 7021 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 7081 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 7141 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 7201 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 7261 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 7321 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 7381 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 7441 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 7501 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 7561 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 7621 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 7681 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 7741 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 7801 AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 7861 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 7921 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 7981 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 8041 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 8101 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 8161 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 8221 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 8281 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 8341 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 8401 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 8461 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 8521 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 8581 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 8641 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 8701 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 8761 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 8821 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 8881 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 8941 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 9001 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???