pCMV-Rrp12-3×FLAG-Neo plasmid

PVT103409

pCMV-Rrp12-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Rrp12(mouse)-3×FLAG-Neo
Alias: NM_199447.2;mKIAA0690
Gene lenght: 3885
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Rrp12-3×FLAG-Neo plasmid Description

pCMV-Rrp12-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Rrp12-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatggg tcgctcgggg aaattgccct 781 ctggtgtctc ggccaagttg aagcgctgga agaaaggcca cagcagcgac agcaaccccg 841 ccacttgccg ccatcgccag gccgctcgga gccgcttctt cagccgcccg tcaggaaaga 901 gcgacctgac tgttgatgct gtgaagttgc ataatgagct gcagtcgggg accctgagcc 961 tgggcaaaag ccaagcccct gagacgacca tggaccaaga tccagaggtg gctttcaccg 1021 agaagtcctc gggcacgttt ctgagtggct tgtctgactg tacaaatgtc actttcagca 1081 aagtgcagcg cttctgggaa tccaactcag ctgctcacaa ggagatctgc gctgtgctgg 1141 ctgctgttac agaggtgatt cgatcccagg gaggcaagga gacggagacc gagtactttg 1201 ctgctctgat gacaacaatg gaagcagtgg agtccccaga gtcactggct gccgttgcat 1261 acctattgaa ccttgtcctg aagcgggtgc caagtcctgt gctcatgaag aagttctctg 1321 atacctccaa agccttcatg gacatcatgt ctgcgcaggc gagcagtggc tccacctctg 1381 ctctccgatg ggtcctctcc tgcctggcca tcctccttcg gaagcaagac ctggaggcct 1441 ggggctatcc catcaccctt caggtgtacc atggactgct gagcttcacc gtgcatgcca 1501 agccgaagat ccgtaaggct gctcagcacg gagtgtgctc agttctcaag ggcagtgact 1561 tcatgtttgg tgaaaaggcc cctgcccatc atcctgctgc cgtctccact gccaagttct 1621 gcatccagga gattgagaag tctggaggtt ccaaagaggc caccaccacg ctgcatatgc 1681 taacactgct gaaggacatg ctgccctgct tcccggaagg cctggtgaag agctgcagtg 1741 agactctgct tcgggtcatg accttgaacc atgtgcttgt gacagcctgt gccatgcagg 1801 ccttccataa cctcttccat gccaagccca gccctagcac cctgtcagca gaactcaacg 1861 cccagatcgt cacggcacta tatgactacg ttcccagtga gaatgattta cagcccctgc 1921 tggcctggct taaggtcatg gagaaagccc acatcaacct ggtcaggctg cagcgagacc 1981 tggggctagg ccatcttgct cgatttttcg gaaccgcagt gacctgtctt ctctcgcctc 2041 attcacaagt ggcggcggct gctacccaga ctctcaagga gatcctgaag gagtgtgtgg 2101 ctccacacat tgctgacatt ggctctgtga cctcttcagc ctcaggtcct ccccagtaca 2161 tcactaagat gttcagggca gtggaagagg gcctcacata caaatttcat gcagcctgga 2221 gctctgtgct gcagctcctg ggggtcttct ttgaagcttg cggaaagcag gcccatcctg 2281 tgatgaagaa gtgcctccag tccctgtgtg acctgcgcct ctctcctcac ttcccccaca 2341 cagcagccct ggaccaggct gtaggggctg cagtgaccag catgggtccc gaggtggtgt 2401 tgcaggccgt gcccttggaa atcgatggct ctgaggagac tctggatttc ccacggagct 2461 ggctgctgcc tgtcatccgg gatcatgttc gggaaaccag gcttggtttc ttcaccactt 2521 acttcttgcc cttggccacc actcttaaga gaaaagcaat ggacctggct caggcaggca 2581 gcacagtgga gtcgaagatt tatgatacac tccagtggca gatctggacc ctcctgcctg 2641 ggttctgcac aaggcccaca gatgtggctg cctccttcaa aggcctggca cggactctgg 2701 gcacagctat caatgaacgc ccagacctga gggtcactgt gtgccaggcc ctacgtaccc 2761 tcatcactaa gggctgtgag gcagaggctg accgagctga agtgagccgc tttgccaaaa 2821 acttcctgcc aatcctcttc aacctgtatg ggcagcctgt tgcagctgga gaggcggcag 2881 cccctcgccg tgctgtgctg gaaaccatca aaacttacct caccataact gaggctcagt 2941 tggtgaatag cttcctggaa aaagccaccg agaaggttct cgatcctgcc agctctgatt 3001 tcaccagatt gtctgtcctg gatctggttg tggctttggc tccttactcg gatgaagctg 3061 ccatcagtaa gctatactct accatccggc cctaccttga gagcaaggtc cacggtgtac 3121 agaagaaggc ctaccgcgtg ctggaggagg tgtgtgccag ttctcagggc ccggcagccc 3181 gcttcgtgca gagccacctg gatgatctga agaagactct gctggactcg cttcggacca 3241 catcctcgcc tgctaaaagg ccccgtttga agtgcctcat acatattgtg aagacgctgt 3301 cggctgagca tgaggagttc atagctgccc tcatcccaga ggtgatcctg tgcaccaagg 3361 aggtgtcagt gggtgcaagg aagagtgcgt ttaccttgct ggtggagatg ggccatgctt 3421 tcctgcgatt tggctccaac caggaagacg ctctgcagcg gtaccttgtc ctcatctatc 3481 ctggccttct gggtgcagtt accacagtta gttgcagcat tttggctctg acccacctct 3541 tgtttgagtt caaagggctg atggggacca gcacagtgga gcagctcctg gagaacgtgt 3601 gcctgctgct ggcctctcga acccgtgatg tggtcaagtc cgcgctaggc ttcatcaagg 3661 ttgctgtggt ggtcatggac gtggtgcacc ttgctaagca tgtgcagcta gtgatggaag 3721 ccattgggaa gttgtcagat gacatgcgac ggcacttccg gatgaagctt cggaacctgt 3781 tcatcaagtt cacccgcaag tttggattcg agttggtgaa gggtctgcta cccgcagaat 3841 accacaaagt cctgataaac atcaggaaag ctgagacccg agccaagaag catcgtgccc 3901 tgagccaggc tgctgtagag gaggaggaag aggaggaaga ggaagaggag cctgtccaga 3961 gcaaaggtga cagcattgag gagattttgg ctgactctga ggatgaggat gaggaagaag 4021 aaagaggccg gggcaaggag cagcggaagc tggcacgaca gaggagccgg gcatggctga 4081 aggaaggtgg tggcgatgag cccctcaact tcctggatcc caaggtggcc catcgagtcc 4141 ttgccaccca gcctgggcct gggcggggca agaagaggga ccatggcttc aagttgagtg 4201 ctgatggccg gctgatcata cgggaggagg aagatggaaa caaggtggag gaagaagatg 4261 gcactaaagg ggaagatgaa gacatgaccg atgcgatgga ggatgccagt gtgagaagta 4321 aaaagaagct taagcggcag agggaggacg aggaggatga gctggagatc ccacctcggt 4381 accaagctgg aggctctggc atccatcgcc ctgtggctaa gaaggctgca cctggagctg 4441 aatacaaggc caagaaggca aaaggcgatg taaagaagaa gggccgcctg gatccctatg 4501 cgtacgttcc actcaacaga tccaagctca accgcaggaa gaaggtgaag ctccaggggc 4561 agtttaaagg tctggtgaaa gccacccagc ggggatccca ggcgggacac aaactccgca 4621 gaaaggatcg gcggcggccc GGATCCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC 4681 ATGACATCGA CTACAAGGAT GACGATGACA AGTAGTGAGG GCCCGATATC TCGACAATCA 4741 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT 4801 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC 4861 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 4921 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG 4981 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC 5041 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG 5101 CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG 5161 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC 5221 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT 5281 TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA ACGGGGGAGG 5341 CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA 5401 AGACAGAATA AAACGCACGG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA 5461 GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC 5521 TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT 5581 CGGGGCGGCA GGCCCTGCCA TAGCAGATCT GCGCAGCTGG GGCTCTAGGG GGTATCCCCA 5641 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC 5701 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC 5761 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT TCCGATTTAG 5821 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC 5881 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG 5941 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 6001 AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 6061 CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 6121 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 6181 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 6241 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 6301 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG 6361 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 6421 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 6481 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 6541 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 6601 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 6661 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 6721 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 6781 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 6841 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 6901 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 6961 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 7021 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 7081 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 7141 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 7201 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 7261 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA CGCCCAACCT 7321 GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT 7381 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC 7441 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 7501 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 7561 TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC 7621 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 7681 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 7741 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 7801 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 7861 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 7921 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 7981 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 8041 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 8101 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 8161 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC 8221 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 8281 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 8341 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 8401 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 8461 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 8521 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 8581 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 8641 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 8701 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 8761 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 8821 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 8881 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 8941 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 9001 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 9061 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 9121 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 9181 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 9241 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 9301 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 9361 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 9421 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 9481 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 9541 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 9601 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 9661 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 9721 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG 9781 ATCGGGAGAT CTCCCGATCC CCTATGGTCG ACTCTCAGTA CAATCTGCTC TGATGCCGCA 9841 TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC 9901 AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???