pCMV-SCN2A-FLAG-Twinstrep-Neo plasmid

PVT112040

pCMV-SCN2A-FLAG-Twinstrep-Neo plasmid Information

Plasmid name: pCMV-SCN2A(human-Toxic-opt)-FLAG-Twinstrep-Neo
Alias: NM_021007.3;BFIC3; BFIS3; BFNIS; DEE11; EA9; EIEE11;HBA; HBSCI; HBSCII; Na(v)1.2; NAC2; Nav1.2; SCN2A1;SCN2A2
Gene lenght: 6015
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SCN2A-FLAG-Twinstrep-Neo plasmid Description

pCMV-SCN2A-FLAG-Twinstrep-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-SCN2A-FLAG-Twinstrep-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTGAATT Cgccaccatg gcacagtcag tgctggtacc gccaggacct 961 gacagcttcc gcttctttac cagggaatcc cttgctgcta ttgaacaacg cattgcagaa 1021 gagaaagcta agagacccaa acaggaacgc aaggatgagg atgatgaaaa tggcccaaag 1081 ccaaacagtg acttggaagc aggaaaatct cttccattta tttatggaga cattcctcca 1141 gagatggtgt cagtgcccct ggaggatctg gacccctact atatcaataa gaaaacgttt 1201 atagtattga ataaagggaa agcaatctct cgattcagtg ccacccctgc cctttacatt 1261 ttaactccct tcaaccctat tagaaaatta gctattaaga ttttggtaca ttctttattc 1321 aatatgctca ttatgtgcac gattcttacc aactgtgtat ttatgaccat gagtaaccct 1381 ccagactgga caaagaatgt ggagtatacc tttacaggaa tttatacttt tgaatcactt 1441 attaaaatac ttgcaagggg cttttgttta gaagatttca catttttacg ggatccatgg 1501 aattggttgg atttcacagt cattactttt gcatatgtga cagagtttgt ggacctgggc 1561 aatgtctcag cgttgagaac attcagagtt ctccgagcat tgaaaacaat ttcagtcatt 1621 ccaggcctga agaccattgt gggggccctg atccagtcag tgaagaagct ttctgatgtc 1681 atgatcttga ctgtgttctg tctaagcgtg tttgcgctaa taggattgca gttgttcatg 1741 ggcaacctac gaaataaatg tttgcaatgg cctccagata attcttcctt tgaaataaat 1801 atcacttcct tctttaacaa ttcattggat gggaatggta ctactttcaa taggacagtg 1861 agcatattta actgggatga atatattgag gataaaagtc acttttattt tttagagggg 1921 caaaatgatg ctctgctttg tggcaacagc tcagatgcag gccagtgtcc tgaaggatac 1981 atctgtgtga aggctggtag aaaccccaac tatggctaca cgagctttga cacctttagt 2041 tgggcctttt tgtccttatt tcgtctcatg actcaagact tctgggaaaa cctttatcaa 2101 ctgacactac gtgctgctgg gaaaacgtac atgatatttt ttgtgctggt cattttcttg 2161 ggctcattct atctaataaa tttgatcttg gctgtggtgg ccatggccta tgaggaacag 2221 aatcaggcca cattggaaga ggctgaacag aaggaagctg aatttcagca gatgctcgaa 2281 cagttgaaaa agcaacaaga agaagctcag gcggcagctg cagccgcatc tgctgaatca 2341 agagacttca gtggtgctgg tgggatagga gttttttcag agagttcttc agtagcatct 2401 aagttgagct ccaaaagtga aaaagagctg aaaaacagaa gaaagaaaaa gaaacagaaa 2461 gaacagtctg gagaagaaga gaaaaatgac agagtccgaa aatcggaatc tgaagacagc 2521 ataagaagaa aaggtttccg tttttccttg gaaggaagta ggctgacata tgaaaagaga 2581 ttttcttctc cacaccagtc cttactgagc atccgtggct cccttttctc tccaagacgc 2641 aacagtaggg cgagcctttt cagcttcaga ggtcgagcaa aggacattgg ctctgagaat 2701 gactttgctg atgatgagca cagcaccttt gaggacaatg acagccgaag agactctctg 2761 ttcgtgccgc acagacatgg agaacggcgc cacagcaatg tcagccaggc cagccgtgcc 2821 tccagggtgc tccccatcct gcccatgaat gggaagatgc atagcgctgt ggactgcaat 2881 ggtgtggtct ccctggtcgg gggcccttct accctcacat ctgctgggca gctcctacca 2941 gagggcacaa ctactgaaac agaaataaga aagagacggt ccagttctta tcatgtttcc 3001 atggatttat tggaagatcc tacatcaagg caaagagcaa tgagtatagc cagtattttg 3061 accaacacca tggaagaact tgaagaatcc agacagaaat gcccaccatg ctggtataaa 3121 tttgctaata tgtgtttgat ttgggactgt tgtaaaccat ggttaaaggt gaaacacctt 3181 gtcaacctgg ttgtaatgga cccatttgtt gacctggcca tcaccatctg cattgtctta 3241 aatacactct tcatggctat ggagcactat cccatgacgg agcagttcag cagtgtactg 3301 tctgttggaa acctggtctt cacagggatc ttcacagcag aaatgtttct caagataatt 3361 gccatggatc catattatta ctttcaagaa ggctggaata tttttgatgg ttttattgtg 3421 agccttagtt taatggaact tggtttggca aatgtggaag gattgtcagt tctccgatca 3481 ttccggctgc tccgagtttt caagttggca aaatcttggc caactctaaa tatgctaatt 3541 aagatcattg gcaattctgt gggggctcta ggaaacctca ccttggtatt ggccatcatc 3601 gtcttcattt ttgctgtggt cggcatgcag ctctttggta agagctacaa agaatgtgtc 3661 tgcaagattt ccaatgattg tgaactccca cgctggcaca tgcatgactt tttccactcc 3721 ttcctgatcg tgttccgcgt gctgtgtgga gagtggatag agaccatgtg ggactgtatg 3781 gaggtcgctg gccaaaccat gtgccttact gtcttcatga tggtcatggt gattggaaat 3841 ctagtggttc tgaacctctt cttggccttg cttttgagtt ccttcagttc tgacaatctt 3901 gctgccactg atgatgataa cgaaatgaat aatctccaga ttgctgtggg aaggatgcag 3961 aaaggaatcg attttgttaa aagaaaaata cgtgaattta ttcagaaagc ctttgttagg 4021 aagcagaaag ctttagatga aattaaaccg cttgaagatc taaataataa aaaagacagc 4081 tgtatttcca accataccac catagaaata ggcaaagacc tcaattatct caaagacgga 4141 aatggaacta ctagtggcat aggcagcagt gtagaaaaat atgtcgtgga tgaaagtgat 4201 tacatgtcat ttataaacaa ccctagcctc actgtgacag taccaattgc tgttggagaa 4261 tctgactttg aaaatttaaa tactgaagaa ttcagcagcg agtcagatat ggaggaaagc 4321 aaagagaagc taaatgcaac tagttcatct gaaggcagca cggttgatat tggagctccc 4381 gccgagggag aacagcctga ggttgaacct gaggaatccc ttgaacctga agcctgtttt 4441 acagaagact gtgtacggaa gttcaagtgt tgtcagataa gcatagaaga aggcaaaggg 4501 aaactctggt ggaatttgag gaaaacatgc tataagatag tggagcacaa ttggttcgaa 4561 accttcattg tcttcatgat tctgctgagc agtggggctc tggcctttga agatatatac 4621 attgagcagc gaaaaaccat taagaccatg ttagaatatg ctgacaaggt tttcacttac 4681 atattcattc tggaaatgct gctaaagtgg gttgcatatg gttttcaagt gtattttacc 4741 aatgcctggt gctggctaga cttcctgatt gttgatgtct cactggttag cttaactgca 4801 aatgccttgg gttactcaga acttggtgcc atcaaatccc tcagaacact aagagctctg 4861 aggccactga gagctttgtc ccggtttgaa ggaatgaggg ttgttgtaaa tgctctttta 4921 ggagccattc catctatcat gaatgtactt ctggtttgtc tgatcttttg gctaatattc 4981 agtatcatgg gagtgaatct ctttgctggc aagttttacc attgtattaa ttacaccact 5041 ggagagatgt ttgatgtaag cgtggtcaac aactacagtg agtgcaaagc tctcattgag 5101 agcaatcaaa ctgccaggtg gaaaaatgtg aaagtaaact ttgataacgt aggacttgga 5161 tatctgtctc tacttcaagt agccacgttt aagggatgga tggatattat gtatgcagct 5221 gttgattcac gaaatgtaga attacaaccc aagtatgaag acaacctgta catgtatctt 5281 tattttgtca tctttattat ttttggttca ttctttacct tgaatctttt cattggtgtc 5341 atcatagata acttcaacca acagaaaaag aagtttggag gtcaagacat ttttatgaca 5401 gaagaacaga agaaatacta caatgcaatg aaaaaactgg gttcaaagaa accacaaaaa 5461 cccatacctc gacctgctaa caaattccaa ggaatggtct ttgattttgt aaccaaacaa 5521 gtctttgata tcagcatcAT GATTTTGATT TGTCTTAACA TGGTTACAAT GATGGTGGAA 5581 ACAGATGACC AATCCCAGGA AATGACCAAC ATTCTGTATT GGATCAACCT GGTGTTTATC 5641 GTGCTCTTCA CCGGCGAGTG CGTGCTGAAA CTCATATCAC TGCGCTACTA CTACTTCACT 5701 ATCGGGTGGA ATATCTTCGA CTTCGTGGTC GTTATCCTGT CTATCGTAGG AATGTTCCTT 5761 GCGGAGCTGA TCGAGAAATA CTTCGTATCC CCGACTCTGT TCCGCGTGAT CAGGTTGGCT 5821 CGAATCGGTA GAATCCTGAG GCTGATTAAG GGCGCCAAGG GCATCAGAAC TCTTCTGTTT 5881 GCACTGATGA TGAGTCTCCC AGCTCTTTTC AACATAGGCC TGCTGCTGTT CCTGGTCATG 5941 TTTATCTATG CCATATTTGG GATGTCTAAT TTCGCGTACG TTAAGCGGGA AGTTGGCATC 6001 GATGACATGT TTAACTTTGA AACCTTCGGG AACTCCATGA TTTGTCTGTT CCAAATCACA 6061 ACATCTGCAG GATGGGACGG ACTGTTGGCT CCCATCCTCA ATTCAGGCCC TCCCGACTGC 6121 GATCCTGATA AGGACCATCC CGGGAGCAGC GTCAAGGGGG ATTGTGGTAA CCCATCAGTG 6181 GGCATCTTCT TTTTTGTGAG TTATATCATC ATCTCCTTCC TGGTAGTGGT GAACATGTAT 6241 ATTGCAGTGA TTCTGGAAAA CTTCTCAGTG GCAACTGAAG AATCAGCTGA ACCCTTGTCC 6301 GAGGATGACT TCGAGATGTT TTATGAAGTG TGGGAAAAAT TTGACCCCGA TGCAACGCAG 6361 TTCATCGAAT TCGCAAAACT GTCCGACTTC GCCGATGCCC TGGACCCCCC TCTCCTTATC 6421 GCAAAGCCCA ACAAGGTGCA ACTCATCGCC atggatctgc ccatggtgag tggtgaccgg 6481 atccactgtc ttgacatctt atttgctttt acaaagcgtg ttttgggtga gagtggagag 6541 atggatgccc ttcgaataca gatggaagag cgattcatgg catcaaaccc ctccaaagtc 6601 tcttatgagc ccattacgac cacgttgaaa cgcaaacaag aggaggtgtc tgctattatt 6661 atccagaggg cttacagacg ctacctcttg aagcaaaaag ttaaaaaggt atcaagtata 6721 tacaagaaag acaaaggcaa agaatgtgat ggaacaccca tcaaagaaga tactctcatt 6781 gataaactga atgagaattc aactccagag aaaaccgata tgacgccttc caccacgtct 6841 ccaccctcgt atgatagtgt gaccaaacca gaaaaagaaa aatttgaaaa agacaaatca 6901 gaaaaggaag acaaagggaa agatatcagg gaaagtaaaa agCTCGAGGA TTACAAGGAT 6961 GACGACGATA AGGGATCCag cgcctggtcc caccctcagt ttgagaaggg cggaggctct 7021 ggcggcggaa gcggaggatc tgcttggagc cacccccagt tcgaaaagtg aTGAGGGCCC 7081 GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC 7141 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA 7201 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG 7261 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG 7321 GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG 7381 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 7441 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 7501 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 7561 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 7621 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 7681 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 7741 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 7801 AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 7861 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG 7921 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC 7981 CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC 8041 ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT 8101 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG 8161 CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG 8221 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG 8281 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG 8341 GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG 8401 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG 8461 TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC 8521 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC 8581 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC 8641 GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC 8701 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG 8761 ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT 8821 TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT 8881 TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC 8941 TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT 9001 TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC 9061 ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG 9121 GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC 9181 CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 9241 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 9301 TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT 9361 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 9421 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 9481 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 9541 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 9601 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 9661 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 9721 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 9781 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 9841 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 9901 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 9961 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 10021 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 10081 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 10141 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 10201 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 10261 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 10321 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 10381 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 10441 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 10501 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 10561 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 10621 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 10681 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 10741 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 10801 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 10861 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 10921 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 10981 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 11041 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 11101 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 11161 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 11221 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 11281 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 11341 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 11401 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 11461 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.