pCMV-SLC5A12-3×FLAG-mCMV-EGFP plasmid

PVT95004

pCMV-SLC5A12-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-SLC5A12(human)-3×FLAG-mCMV-EGFP
Alias: NM_178498.4;SMCT2
Gene lenght: 1854bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SLC5A12-3×FLAG-mCMV-EGFP plasmid Description

pCMV-SLC5A12-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-SLC5A12-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatggag gtgaagaact ttgcagtttg 661 ggattatgtt gtatttgcag ccctcttttt catttcctct ggaattgggg tgttctttgc 721 cattaaggag agaaaaaagg caacttcccg agagttcctg gttgggggaa ggcaaatgag 781 ctttggccct gtcggcttgt ctctgacagc cagcttcatg tcagctgtca cggtcctggg 841 gaccccttct gaagtctacc gctttggggc atccttccta gtcttcttca ttgcttacct 901 atttgtcatc ctcttaacat cagagctctt tctccctgtg ttctacagat ctggtatcac 961 cagcacttat gagtacttac aactacgatt caacaaacca gttcgctatg ctgccacggt 1021 catctacatt gtacagacga ttctctacac aggagtggtg gtgtatgctc ctgccctggc 1081 actcaatcaa gtgactgggt ttgatctctg gggctctgtg tttgcaacag gaattgtttg 1141 cacattctac tgtaccctgg gaggattaaa agcagtggtg tggacagatg catttcagat 1201 ggttgtcatg attgtgggct tcttaacggt tctcattcaa ggatcaactc atgctggggg 1261 attccacaat gtattagagc aatcaacaaa tggatctcga ctacatatat ttgactttga 1321 tgtagatcct ctcaggcgac acactttttg gactatcaca gtgggaggaa cttttacttg 1381 gctcggaatc tatggggtca atcaatcaac tattcagcga tgcatctctt gcaaaacaga 1441 aaagcatgct aagcttgcct tgtattttaa cttgctgggt ctctggatca ttctggtgtg 1501 tgctgtcttc tctggcttaa tcatgtactc tcactttaaa gactgtgacc cttggacttc 1561 tggcatcatc tcagcaccag accagctgat gccgtacttt gtcatggaga tatttgccac 1621 aatgccagga ctgccaggac tttttgtggc ttgtgccttc agtggaactc tgagcaccgt 1681 ggcttccagc atcaatgcct tggcaacagt gacctttgag gattttgtca agagctgttt 1741 tcctcatctc tccgacaagc tgagcacctg gatcagtaaa ggcttatgtc tcttatttgg 1801 cgtgatgtgt acctctatgg ctgtggctgc atctgtcatg ggaggtgttg tgcaggcttc 1861 cctcagcatt cacggcatgt gtggaggacc aatgctgggc ttattctccc tgggaatcgt 1921 gttccctttt gtgaattgga agggtgcact aggaggtctt cttactggaa tcaccttgtc 1981 attttgggtg gccattgggg ccttcattta ccctgcacca gcctctaaga catggccttt 2041 gcctctgtca acagaccaat gtatcaaatc aaatgtgaca gcaacagggc ctccagtact 2101 atccagcaga cctggaatag ctgatacctg gtactcgatc tcctaccttt actacagtgc 2161 agtgggctgc ttaggatgca ttgttgctgg agtaatcatc agcctcataa caggtcgcca 2221 aagaggtgag gatattcaac cactgttaat tagaccagtt tgtaatttat tttgcttttg 2281 gtctaagaag tacaaaacac tatgctggtg tggagttcag catgacagtg ggacagagca 2341 ggaaaacctt gagaatggca gtgcccggaa acagggggct gaatctgtct tacagaacgg 2401 actcagaaga gaaagcctgg tacatgttcc aggctatgat cctaaggaca aaagctacaa 2461 caatatggca tttgagacta cccatttcAA GCTTACGCGT GACTACAAAG ACCATGACGG 2521 TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTGAG GATCCGGGCC 2581 CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 2641 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 2701 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 2761 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT 2821 GGGCTCTATG GTCTAGAGAT ATACTGAGTC ATTAGGGACT TTCCAATGGG TTTTGCCCAG 2881 TACATAAGGT CAATAGGGGT GAATCAACAG GAAAGTCCCA TTGGAGCCAA GTACACTGAG 2941 TCAATAGGGA CTTTCCATTG GGTTTTGCCC AGTACAAAAG GTCAATAGGG GGTGAGTCAA 3001 TGGGTTTTTC CCATTATTGG CACGTACATA AGGTCAATAG GGGTGAGTCA TTGGGTTTTT 3061 CCAGCCAaTT TAATTAAAAC GCCATGTACT TTCCCACCAT TGACGTCAAT GGGCTATTGA 3121 AACTAATGCA ACGTGACCTT TAAACGGTAC TTTCCCATAG CTGATTAATG GGAAAGTACC 3181 GTTCTCGAGC CAATACACGT CAATGGGAAG TGAAAGGGCA GCCAAAACGT AACACCGCCC 3241 CGGTTTTCCC CTGGAAATTC CATATTGGCA CGCATTCTAT TGGCTGAGCT GCGTTCTACG 3301 TGGGTATAAG AGGCGCGACC AGCGTCGGTA CCGTCGCAGT CTTCGGTCTG ACCACCGTAG 3361 AACGCAGATC accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 3421 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 3481 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 3541 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 3601 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 3661 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 3721 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 3781 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 3841 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 3901 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 3961 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 4021 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 4081 gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac 4141 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 4201 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 4261 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 4321 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa 4381 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 4441 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 4501 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 4561 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 4621 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 4681 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 4741 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 4801 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 4861 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 4921 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt 4981 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 5041 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 5101 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 5161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 5221 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 5281 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg 5341 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 5401 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 5461 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 5521 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 5581 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 5641 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 5701 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 5761 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 5821 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 5881 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 5941 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 6001 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 6061 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 6121 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 6181 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 6241 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 6301 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac 6361 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt 6421 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc 6481 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc 6541 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat 6601 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 6661 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 6721 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 6781 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 6841 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 6901 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 6961 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 7021 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 7081 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 7141 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 7201 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 7261 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 7321 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 7381 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???