pCMV-SPORT6-Hdlbp Plasmid

Alias:BC035301.1
Gene length:3807bp
Host: E.coli
Use(s): Gene template
Fragment Type: cDNA
Fragmented species: Mouse
Prokaryotic resistance: Amp
Screening Markers:
Fluorescent labeling:
Promoter:
replicon: pUC
Copy Number:
Competent cells: DH5a
Temperature: 37 Degrees
Back Bones:pCMV-SPORT6
Forward primer:M13R：CAGGAAACAGCTATGACC
Reverse primer: M13F：TGTAAAACGACGGCCAGTInduction method:
Sequence:
GCTGTGCTAGGCAGGCTGGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGTCACACTCACGATAAAAGCTGGCAGGCTGACAGAGGCAACCTCAGGACGGACTTTCTGGCTACTGACCATTTTGCTGTGTCTTACTAGGATCGTGTGTGGACGTGAGATCAACCATGAGCTCCGTTGCAGTTTTGACCCAAGAGAGTTTTGCTGAACATCGAAGTGGGCTGGTTCCACAACAAATCAAAGTTGCCACCTTAAATTCAGAAGAGGAAAATGACCCTCCAACCTACAAGGATGCCTTCCCTCCACTTCCTGAGAAAGCAGCTTGCTTGGAGAGTGCCCAAGAACCTGCCGGAGCCTGGAGTAACAAGATCCGGCCCATCAAGGCCTCTGTCATCACTCAGGTGTTTCATGTACCCCTGGAAGAGAGAAAATATAAGGACATGAACCAGTTTGGAGAGGGTGAACAAGCAAAAATCTGCCTTGAGATCATGCAGAGGACTGGTGCTCACCTGGAACTCTCTCTGGCTAAAGATCAAGGTCTCTCCATCATGGTCTCAGGGAAGCTGGATGCCGTCATGAAAGCCCGCAAAGACATTGTTGCTAGACTACAGACACAGGCCTCAGCAACTGTTCCCATTCCTAAAGAGCACCATCGCTTTGTTATTGGCAAAAACGGAGAGAAACTGCAAGACTTAGAACTAAAAACTGCAACCAAAATCCAGATCCCACGCCCAGATGACCCCAGTAATCAGATCAAGATCACTGGCACCAAAGAAGGCATCGAGAAAGCTCGCCATGAAGTCCTCCTAATCTCTGCTGAGCAGGACAAGCGTGCTGTAGAGAGGCTAGAAGTGGAGAAGGCTTTCCACCCCTTCATCGCTGGGCCGTACAACAGACTTGTGGGTGAAATCATGCAGGAGACAGGGACGCGAATCAACATCCCTCCACCCAGTGTTAACCGTACAGAAATCGTCTTCACTGGAGAGAAGGAGCAGCTGGCTCAGGCTGTGGCTCGCATTAAGAAGATTTATGAGGAAAAGAAGAAGAAGACTACAACCATTGCGGTAGAGGTGAAGAAGTCTCAGCACAAGTATGTTATTGGGCCAAAGGGCAATTCACTGCAAGAAATCCTGGAAAGAACTGGAGTTTCTGTTGAGATCCCACCCTCCGACAGCATCTCTGAGACTGTGATACTTAGAGGCGAACCTGAAAAGTTGGGACAGGCGTTGACTGAAGTCTATGCCAAGGCCAACAGTTTTACAGTCTCCTCAGTCTCTGCCCCTTCCTGGCTTCATCGTTTCATCATTGGTAAGAAAGGGCAGAACCTGGCCAAAATCACTCAGCAAATGCCAAAGGTTCACATTGAGTTCACAGAAGGTGAGGACAAGATCACCCTGGAGGGCCCCACAGAGGATGTGAATGTAGCCCAAGAACAGATTGAAGGAATGGTCAAAGACCTGATTAATAGGATGGACTATGTAGAGATCAACATTGACCATAAATTCCACAGACACCTCATTGGAAAGAGCGGAGCCAACATCAACAGAATCAAAGACCAGTACAAAGTGTCTGTGCGCATCCCCCCTGACAGTGAAAAGAGTAACCTGATCCGAATTGAGGGAGACCCACAGGGTGTTCAGCAGGCCAAGCGGGAGCTGCTGGAGCTTGCTTCTCGCATGGAAAATGAGCGCACCAAGGATCTAATCATCGAGCAAAGATTTCATCGCACAATCATTGGGCAGAAAGGTGAACGGATTCGAGAAATTCGTGACAAATTCCCAGAGGTTATCATCAACTTTCCAGACCCAGCACAAAAAAGTGATATTGTACAACTCAGAGGCCCCAAGAATGAGGTGGAGAAGTGCACTAAGTATATGCAGAAGATGGTCGCAGACCTGGTGGAAAATAGCTATTCAATTTCTGTACCAATCTTCAAACAGTTTCACAAGAATATCATCGGGAAAGGAGGCGCAAACATTAAAAAGATCCGTGAAGAGAGCAACACCAAGATTGACCTTCCTGCAGAGAACAGCAACTCTGAGACCATTATTATCACTGGCAAGCGAGCCAACTGTGAAGCTGCCCGCAGCCGGATTCTGTCTATCCAGAAAGATCTGGCCAACATAGCTGAAGTGGAGGTCTCAATTCCTGCCAAACTACACAACTCTCTCATTGGCACAAAGGGCCGTCTGATCCGCTCCATCATGGAGGAATGTGGTGGCGTCCATATTCACTTTCCTGTGGAAGGTTCTGGAAGCGACACTGTTGTCATCAGGGGTCCTTCCTCAGATGTAGAAAAGGCCAAGAAGCAGCTCCTGCACCTGGCTGAGGAGAAGCAAACCAAGAGTTTTACTGTGGACATCCGAGCCAAGCCAGAATACCACAAATTCCTCATTGGCAAAGGGGGAGGTAAAATCCGAAAGGTGCGTGACAGTACTGGAGCCCGCATCATATTTCCGGCTGCTGAAGATAAGGACCAGGACCTGATCACAATCATAGGAAAGGAGGATGCTGTCCGTGAGGCCCAAAAGGAGCTGGAAGCATTGATCCAGAACCTGGAGAATGTGGTTGAAGACTATATGCTGGTGGACCCCAAACACCACCGCCATTTTGTCATCCGAAGAGGACAAGTTTTACGAGAAATTGCTGAAGAGTATGGCGGGGTGATGGTCAGCTTCCCACGCTCTGGCACGCAGAGTGATAAAGTCACTCTCAAGGGTGCCAAGGACTGTGTGGAGGCAGCCAAGAAACGCATTCAGGAAATCATTGAGGATCTGGAAGCTCAAGTGACAGTGGAATGTGCTATACCCCAGAAGTTTCACCGATCGGTCATGGGTCCCAAAGGTTCAAGAATCCAGCAGATCACTCGGGACTACAATGTTCAAATCAAGTTCCCAGATAGAGAGGAAAATCCAGTTCACAGTGTAGAGCCTTCCATCCAGGAGAATGGAGATGAGGCTGGAGAGGGCAGAGAGGCCAAGGAGACCGACCCTGGCTCTCCCAGGAGGTGTGACATCATAATCATCTCTGGCCGGAAAGAGAAATGCGAGGCTGCCAAGGAAGCCCTGGAGGCACTGGTTCCAGTCACCATCGAGGTAGAGGTACCCTTTGATCTTCACCGCTACATCATTGGGCAAAAAGGAAGTGGAATCCGCAAGATGATGGATGAGTTTGAGGTGAACATCCATGTACCAGCCCCCGAGCTGCAGTCAGACACCATCGCCATCACTGGCCTTGCAGCAAATTTGGACAGGGCTAAGGCGGGGCTACTGGATCGAGTAAAGGAGCTGCAGGCTGAGCAGGAAGATCGGGCTTTAAGGAGTTTTAAGCTGAGTGTCACTGTAGACCCCAAATACCATCCCAAAATTATCGGGAGAAAAGGAGCAGTGATCACCCAGATCCGCTTGGAACATGAAGTGAACATCCAATTTCCTGATAAGGATGATGGGAACCAGCCCCAGGACCAAATCACCATCACAGGGTATGAGAAGAACACTGAAGCTGCCCGTGATGCTATCCTGAAAATTGTGGGTGAGCTTGAGCAGATGGTTTCTGAGGATGTTCCACTGGATCACCGTGTGCACGCTCGAATCATTGGTGCCCGGGGCAAAGCCATCCGGAAAATAATGGATGAATTCAAGGTGGACATTCGCTTCCCACAGAGTGGAGCGCCAGACCCCAATTGCGTCACTGTGACAGGACTCCCAGAGAATGTGGAGGAAGCTATCGACCATATCCTCAACCTAGAAGAGGAATATCTGGCAGATGTGGTGGACAGTGAGGCACTGCAAGTTTACATGAAGCCCCCAGCACATGAAGAGTCCAGGGCACCATCCAAAGGCTTTGTGGTGCGAGATGCTCCCTGGACCTCCAACAGCAGTGAGAAGGCTCCTGATATGAGCAGCTCTGAAGAATTTCCCAGCTTTGGGGCTCAAGTGGCCCCCAAGACCCTCCCTTGGGGCCCCAAACGATAATGATCAAAAAGAAACCTTTCCAGCCTGCTGCCCAAACCCAACCACACAATGGTTTGTCTCAATCTGACCCAGCAGCTGGACCCTCGTAAATTGTTGATGCTCTTTCCCCTCCCCGAGGTCTCGCAGCAAAGCCTGGTGGGCTGGCCTTGTGTGGCTGCTTCTCCAGGCCTGGTCAGCACCCAGCAGGCTCAGGGTCTGCTCCTGATGCTGTGCTCTGGGACAGGCACGCCACTGTGTGAAACACTAAGCAAGGTAATCGAGCATTTCGTGATCACAGACTCCAGCTTCCTGGTCCACCCAGCATGTAGTCAGCACTCTGACCTTCACACCAGAGCTCCACAGCGGCTAGGAGTTGACTTCCTGTGTCATGACCTCAGGAAATAAATTTCCTTGACTTTATAAAAGCCAAAAAAAAAAAAAAAGGGCGGCCGCTCTAGAGTATCCCTCGAGGGGCCCAAGCTTACGCGTACCCAGCTTTCTTGTACAAAGTGGTCCCTATAGTGAGTCGTATTATAAGCTAGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACTGCTAGCTTGGGATCTTTGTGAAGGAACCTTACTTCTGTGGTGTGACATAATTGGACAAACTACCTACAGAGATTTAAAGCTCTAAGGTAAATTTTC
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Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
4.mouse;