pCMV-Stag2-3×FLAG-Neo plasmid

PVT92654

pCMV-Stag2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Stag2(mouse-opt-mouse)-3×FLAG-Neo
Alias: NM_021465.4;9230105L23Rik; B230112I07Rik; SA-2; SAP2
Gene lenght: 3693bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-Stag2-3×FLAG-Neo plasmid Description

pCMV-Stag2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Stag2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgat agcagctcca gaaataccaa 781 ctgattttaa tctactgcag gagtcagaga cacacttttc ttctgacaca gattttgaag 841 acattgaagg aaaaaatcag aagcaaggca aaggcaaaac ttgtaaaaaa ggcaaaaagg 901 gtccagcaga aaagggtaaa agtggaaatg gaggaggaaa acctccttct ggctcaaacc 961 gaatgaacgg tcatcatcaa cagaatggag ttgaaaacat gatgttgttc gaagttgtta 1021 aaatgggcaa gagtgctatg cagtcggtgg tagatgactg gatcgagtcc tacaagcatg 1081 accgggacat agcgcttctt gaccttatca atttctttat tcagtgctca ggctgcaaag 1141 gagtcgtcac agcagagatg ttcagacata tgcagaactc tgagataatt cgaaaaatga 1201 cagaagaatt tgacgaggat agtggggatt acccacttac catggctggt cctcagtgga 1261 agaagttcaa gtccagcttc tgtgagttca ttggtgtgtt agtacggcag tgtcaataca 1321 gtatcatata tgatgagtac atgatggata ctgtcatttc acttcttact ggattgtctg 1381 actcccaagt cagagcattt cgacatacaa gcaccctggc agctatgaag ttgatgactg 1441 ctttggtaaa tgtggcacta aatcttagca ttaatatgga taacacacaa agacagtacg 1501 aggcagaacg gaataaaatg attggaaaac gagctaacga aaggctagaa ctcctgttac 1561 aaaagcgtaa agagcttcag gaaaatcaag atgagataga aaatatgatg aatgcaatat 1621 ttaaaggagt gtttgtacat agatacaggg atgcaatagc tgaaatcaga gctatatgca 1681 ttgaagagat tggcatttgg atgaagatgt atagtgatgc cttccttaat gacagctatt 1741 taaaatatgt tggttggact atgcatgata agcaaggtga agtcagactc aaatgtctta 1801 ctgctttaca agggctttac tataacaaag aacttaattc caaactggaa ctctttacca 1861 gtcgattcaa ggatagaatt gtgtctatga cccttgacaa agaatatgat gttgcagtac 1921 aagcaataaa gttactcact cttgttttac agagtagtga agaagttcta actgcagaag 1981 attgtgaaaa tgtctatcat ctggtttatt cagctcaccg gccagtagca gttgcagctg 2041 gagaatttct ctacaaaaag ctcttcagcc gcagagaccc agaagaggat gggctgatga 2101 aaagaagggg aaggcaaggc ccgaatgcca accttgtgaa gactttggtg tttttctttt 2161 tggaaagtga gttacacgag catgcagctt accttgtgga cagcATGTGG GACTGCGCTA 2221 CAGAACTCTT GAAAGATTGG GAATGCATGA ATTCTCTCCT GTTGGAAGAA CCCCTGAGCG 2281 GAGAGGAGGC ACTCACTGAT CGCCAGGAAA GTGCTCTTAT CGAGATCATG TTGTGCACTA 2341 TTAGACAGGC AGCAGAATGT CATCCCCCTG TTGGAAGAGG CACAGGGAAA CGCGTGCTGA 2401 CAGCAAAGGA AAAAAAGACC CAGCTGGACG ACAGGACCAG GATTACTGAA TTGTTCGCCG 2461 TCGCCCTGCC TCAGCTGTTG GCCAAGTACA GTGTGGACGC CGAGAAAGTG ACTAATCTGC 2521 TGCAGCTTCC TCAATATTTC GACCTGGAGA TCTATACCAC TGGAAGGCTC GAGAAACATC 2581 TGGACGCACT GCTGAGGCAG ATCAGGAATA TCGTCGAAAA ACACACCGAC ACAGACGTTT 2641 TGGAGGCTTG CTCCAAAACT TACCATGCAC TTTGCAACGA AGAGTTCACA ATCTTCAACC 2701 GCGTCGACAT ATCTAGGTCC CAGTTGATCG ACGAACTGGC TGATAAGTTC AATAGGCTGC 2761 TTGAGGACTT TCTGCAGGAG GGAGAGGAAC CCGATGAGGA TGATGCCTAC CAGGTGCTCT 2821 CCACCCTTAA AAGAATCACA GCATTCCACA ACGCCCATGA TTTGAGCAAG TGGGACTTGT 2881 TCGCATGCAA CTATAAGCTC CTGAAGACAG GAATCGAAAA CGGTGATATG CCCGAACAGA 2941 TTGTAATCCA TGCCTTGCAG TGCGCACATT ACGTCATACT GTGGCAGCTT GCAAAGATTA 3001 CCGAAAGTAC TAGTACCAAG GAAGACCTCC TGCGGCTCAA GAAACAAATG CGGGTATTCT 3061 GTCAGATTTG TCAGCACTAT TTGACCAATG TCAACACTAC CGTGAAGGAA CAAGCCTTCA 3121 CAATTCTGTG CGATATTCTG ATGATCTTCA GCCATCAGAT CATGTCCGGT GGCCGAGACa 3181 tgttagagcc attagtttat actcctgatt cttcattgca gtctgagttg ctcagcttta 3241 ttttggatca tgtcttcatt gaacaggatg atgatagtaa tagtgcagat ggtcagcaag 3301 aggatgaagc cagcaaaatt gaagctttgc acaagaggag aaatttactt gcagcatttt 3361 gtaagctaat tgtatatacc gtggtggaga tgaacacagc tgcagatatc ttcaagcagt 3421 atatgaagta ttataatgat tatggcgata tcatcaaaga aacaatgagt aaaacaaggc 3481 agattgacaa aattcagtgt gctaagaccc ttattctcag tttgcagcag ctttttaatg 3541 agatgataca agagaatggc tataattttg atagatcatc ctcaacattt agtggcataa 3601 aagaacttgc tcggcgtttt gctttaactt ttggacttga tcaattgaaa accagagaag 3661 ccattgccat gctccacaaa gatggtatag aatttgcttt taaggagcct aatccacaag 3721 gggagagcca tccaccttta aacttggcat ttcttgatat tctcagtgaa ttttcttcta 3781 aactgctccg acaggataaa agaacagtgt atgtttattt ggaaaagttc atgacctttc 3841 agatgtcact ccgaagagaa gatgtatggc ttcctctgat gtcttaccga aattctttgc 3901 tagctggtgg tgatgatgat actatgtcag tcattagtgg aatgagcagt cggggatcaa 3961 ctgtacggag taaaaaatca aaaccatcta caggaaagcg gaaagtggtt gaaggcatgc 4021 aactagcact ccctgaagaa agcagtagca gtgacagtat gtggttgagc agagagcaaa 4081 cactgcacac ccctgtcatg atgcagacac cacagctcac ctccactatt atgagagaac 4141 cgaaaaggtt acggcctgaa gatagcttca tgagtgtcta tccgatgcag gcagaacacc 4201 atcaaacgcc tctggattat aatcggcgtg gtacaagcct aatggaagat gatgaagagc 4261 caattgttga agatgttatg atgtcctcag aaggaaggat tgaggatctt aatgagggaa 4321 tggactttga caccatggat atagacttgc caccatcaaa gaatagaaga gagagaacag 4381 aactgaagcc cgatttcttt gatccagctt caattatgga tgaatcagtt cttggggtgt 4441 caatgtttGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT 4501 ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA 4561 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 4621 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 4681 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 4741 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 4801 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 4861 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 4921 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG 4981 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 5041 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 5101 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA 5161 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA 5221 ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC 5281 TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC 5341 ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG 5401 CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 5461 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 5521 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 5581 TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 5641 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 5701 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 5761 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG 5821 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 5881 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5941 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 6001 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 6061 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 6121 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 6181 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 6241 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 6301 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 6361 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 6421 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 6481 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 6541 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 6601 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 6661 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 6721 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 6781 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 6841 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 6901 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 6961 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 7021 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 7081 GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA 7141 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 7201 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT 7261 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 7321 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 7381 TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 7441 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 7501 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 7561 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 7621 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 7681 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 7741 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 7801 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 7861 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 7921 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 7981 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA 8041 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 8101 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 8161 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 8221 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 8281 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 8341 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 8401 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 8461 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 8521 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 8581 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 8641 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 8701 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 8761 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 8821 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8881 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8941 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 9001 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 9061 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 9121 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 9181 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 9241 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 9301 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 9361 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 9421 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 9481 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 9541 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 9601 CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 9661 TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT 9721 ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???