pCMV-STAT2-EGFP-Neo plasmid

PVT97768

pCMV-STAT2-EGFP-Neo plasmid Information

Plasmid name: pCMV-STAT2(human)-EGFP-Neo
Alias: NM_005419.4;IMD44; ISGF-3; P113; PTORCH3; STAT113
Gene lenght: 2553bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-STAT2-EGFP-Neo plasmid Description

pCMV-STAT2-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-STAT2-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatgg cgcagtggga aatgctgcag aatcttgaca 961 gcccctttca ggatcagctg caccagcttt actcgcacag cctcctgcct gtggacattc 1021 gacagtactt ggctgtctgg attgaagacc agaactggca ggaagctgca cttgggagtg 1081 atgattccaa ggctaccatg ctattcttcc acttcttgga tcagctgaac tatgagtgtg 1141 gccgttgcag ccaggaccca gagtccttgt tgctgcagca caatttgcgg aaattctgcc 1201 gggacattca gcccttttcc caggatccta cccagttggc tgagatgatc tttaacctcc 1261 ttctggaaga aaaaagaatt ttgatccagg ctcagagggc ccaattggaa caaggagagc 1321 cagttctcga aacacctgtg gagagccagc aacatgagat tgaatcccgg atcctggatt 1381 taagggctat gatggagaag ctggtaaaat ccatcagcca actgaaagac cagcaggatg 1441 tcttctgctt ccgatataag atccaggcca aagggaagac accctctctg gacccccatc 1501 agaccaaaga gcagaagatt ctgcaggaaa ctctcaatga actggacaaa aggagaaagg 1561 aggtgctgga tgcctccaaa gcactgctag gccgattaac taccctaatc gagctactgc 1621 tgccaaagtt ggaggagtgg aaggcccagc agcaaaaagc ctgcatcaga gctcccattg 1681 accacgggtt ggaacagctg gagacatggt tcacagctgg agcaaagctg ttgtttcacc 1741 tgaggcagct gctgaaggag ctgaagggac tgagttgcct ggttagctat caggatgacc 1801 ctctgaccaa aggggtggac ctacgcaacg cccaggtcac agagttgcta cagcgtctgc 1861 tccacagagc ctttgtggta gaaacccagc cctgcatgcc ccaaactccc catcgacccc 1921 tcatcctcaa gactggcagc aagttcaccg tccgaacaag gctgctggtg agactccagg 1981 aaggcaatga gtcactgact gtggaagtct ccattgacag gaatcctcct caattacaag 2041 gcttccggaa gttcaacatt ctgacttcaa accagaaaac tttgaccccc gagaaggggc 2101 agagtcaggg tttgatttgg gactttggtt acctgactct ggtggagcaa cgttcaggtg 2161 gttcaggaaa gggcagcaat aaggggccac taggtgtgac agaggaactg cacatcatca 2221 gcttcacggt caaatatacc taccagggtc tgaagcagga gctgaaaacg gacaccctcc 2281 ctgtggtgat tatttccaac atgaaccagc tctcaattgc ctgggcttca gttctctggt 2341 tcaatttgct cagcccaaac cttcagaacc agcagttctt ctccaacccc cccaaggccc 2401 cctggagctt gctgggccct gctctcagtt ggcagttctc ctcctatgtt ggccgaggcc 2461 tcaactcaga ccagctgagc atgctgagaa acaagctgtt cgggcagaac tgtaggactg 2521 aggatccatt attgtcctgg gctgacttca ctaagcgaga gagccctcct ggcaagttac 2581 cattctggac atggctggac aaaattctgg agttggtaca tgaccacctg aaggatctct 2641 ggaatgatgg acgcatcatg ggctttgtga gtcggagcca ggagcgccgg ctgctgaaga 2701 agaccatgtc tggcaccttt ctactgcgct tcagtgaatc gtcagaaggg ggcattacct 2761 gctcctgggt ggagcaccag gatgatgaca aggtgctcat ctactctgtg caaccgtaca 2821 cgaaggaggt gctgcagtca ctcccgctga ctgaaatcat ccgccattac cagttgctca 2881 ctgaggagaa tatacctgaa aacccactgc gcttcctcta tccccgaatc ccccgggatg 2941 aagcttttgg gtgctactac caggagaaag ttaatctcca ggaacggagg aaatacctga 3001 aacacaggct cattgtggtc tctaatagac aggtggatga actgcaacaa ccgctggagc 3061 ttaagccaga gccagagctg gagtcattag agctggaact agggctggtg ccagagccag 3121 agctcagcct ggacttagag ccactgctga aggcagggct ggatctgggg ccagagctag 3181 agtctgtgct ggagtccact ctggagcctg tgatagagcc cacactatgc atggtatcac 3241 aaacagtgcc agagccagac caaggacctg tatcacagcc agtgccagag ccagatttgc 3301 cctgtgatct gagacatttg aacactgagc caatggaaat cttcagaaac tgtgtaaaga 3361 ttgaagaaat catgccgaat ggtgacccac tgttggctgg ccagaacacc gtggatgagg 3421 tttacgtctc ccgccccagc cacttctaca ctgatggacc cttgatgcct tctgacttcc 3481 tctcgagtgg cggtggagga agcggcggag gtggatctat ggtgagcaag ggcgaggagc 3541 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 3601 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 3661 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 3721 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 3781 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 3841 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 3901 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 3961 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 4021 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 4081 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 4141 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 4201 ccgggatcac tctcggcatg gacgagctgt acaagtagtc tagagggccc gtttaaaccc 4261 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 4321 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 4381 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 4441 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 4501 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 4561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 4621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 4681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 4741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 4801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 4861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 4921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 4981 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 5041 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 5101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 5161 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 5221 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 5281 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 5341 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 5401 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 5461 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 5521 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 5581 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 5641 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 5701 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 5761 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 5821 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 5881 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 5941 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 6001 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 6061 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 6121 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 6181 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 6241 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 6301 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 6361 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 6421 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 6481 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 6541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 6601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 6661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 6721 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 6781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 6841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 6901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 6961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 7021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 7081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 7141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 7201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 7261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 7321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 7381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 7441 caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 7501 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 7561 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 7621 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 7681 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 7741 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 7801 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 7861 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 7921 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 7981 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 8041 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 8101 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 8161 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 8221 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 8281 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 8341 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 8401 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 8461 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 8521 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 8581 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 8641 ccgcgcacat ttccccgaaa agtgccacct gacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???