pCMV-T7-3×FLAG-Igf2bp1-Neo plasmid

PVT101012

pCMV-T7-3×FLAG-Igf2bp1-Neo plasmid Information

Plasmid name: pCMV-T7-3×FLAG-Igf2bp1(mouse)-Neo
Alias: NM_009951.4;CRD-BP; Crdbp; D030026A21Rik; D11Moh40e; D11Moh45; IMP1; mir-3063; Neilsen; ZBP-1; Zbp1
Gene lenght: 1731bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-T7-3×FLAG-Igf2bp1-Neo plasmid Description

pCMV-T7-3×FLAG-Igf2bp1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-T7-3×FLAG-Igf2bp1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atgaacaagc tttacatcgg caacctcaac 1021 gagagtgtga cccccgcaga cttggagaaa gtattcgcgg agcacaagat ctcctacagc 1081 ggccagttct tggtcaaatc cggctacgcc ttcgtggatt gccccgacga gcactgggcg 1141 atgaaggcca tcgaaacttt ctcggggaaa gtagaactgc aaggaaaacg tctagagatt 1201 gaacactcag tccccaaaaa acaaaggagt cggaaaatac agatccgcaa tattccacct 1261 cagctccgat gggaagtgct agatagcctg ctggctcagt acggtacagt ggagaactgt 1321 gagcaagtga acactgaaag tgagacagct gtggtcaacg tcacctactc taaccgggag 1381 cagaccaggc aagctatcat gaagctaaat ggccatcaac tggagaacca tgccctgaag 1441 gtctcctaca tacctgatga gcagataacg caaggtcctg agaatgggcg tcgtggaggc 1501 tttgggtctc ggggccagcc ccggcaaggg tcgcccgtgg cagcaggggc tccagccaag 1561 cagcagccag tggacatccc tctccggctc ctggtgccta cgcagtatgt aggcgctatc 1621 attggcaagg agggtgccac catccgaaac atcacaaaac agacgcagtc caaaatagac 1681 gtgcatagga aggagaatgc gggcgctgcg gagaaggcca tcagcgtgca ttcaacccct 1741 gaaggctgct cctccgcgtg caagatgatc ttggagatta tgcacaagga ggcaaaggac 1801 accaaaacgg cagatgaagt tcccctgaag atcctggctc ataacaactt cgtcgggcga 1861 ctcattggca aggaagggcg gaacctgaag aaggtggagc aggacacaga gacgaagatc 1921 accatctcat cgctccagga cctcacgctc tataaccctg agaggaccat cactgtgaag 1981 ggcgccattg agaactgttg cagggccgag caggagatca tgaagaaagt tcgagaggct 2041 tacgagaacg acgtggccgc catgagcttg cagtcccacc tcatccctgg gcttaacctg 2101 gctgctgtag gtctcttccc agcttcatcc agcgctgtcc ctcctcctcc cagcagtgtc 2161 accggggctg ctccctatag ctccttcatg caggctccgg agcaggagat ggtacaagtg 2221 ttcatccccg cccaggctgt gggcgccatc attggcaaga agggccagca catcaaacaa 2281 ctctcccgct tcgccagcgc ctccatcaag attgcaccac cagaaacacc tgactccaaa 2341 gttcgaatgg tcgtcatcac tggaccccca gaggctcagt tcaaggccca gggaagaatc 2401 tatggcaaac taaaagaaga gaatttcttt ggtcccaagg aggaagtaaa gctagagacc 2461 cacatacggg ttccggcttc agcagccggc cgtgtcatcg gcaaaggcgg caaaacggtg 2521 aatgagctgc agaacttgac cgcagctgag gtggtagtgc caagagacca gaccccggat 2581 gagaacgacc aagtcattgt taagatcatc ggacatttct atgccagcca gatggctcag 2641 cggaagatcc gagacatcct ggctcaagtt aagcaacagc accagaaggg acagagcaac 2701 ctggcccagg ctcggaggaa gTAgaattct gcagatatcc agcacagtgg cggccgctcg 2761 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 2821 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 2881 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 2941 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 3001 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 3061 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 3121 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 3181 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 3241 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 3301 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 3361 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 3421 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 3481 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 3541 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 3601 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 3661 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 3721 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 3781 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 3841 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc 3901 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 3961 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 4021 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 4081 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 4141 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 4201 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 4261 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 4321 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 4381 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 4441 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 4501 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 4561 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 4621 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4681 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 4741 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4801 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4861 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 4921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 4981 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 5041 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 5101 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 5161 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 5221 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 5281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5341 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5401 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5461 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5521 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5581 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5641 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 5701 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 5761 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 5821 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 5881 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 5941 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtGGTtt ttttgtttgc 6001 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6061 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6121 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6181 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6241 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6301 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6361 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6421 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6481 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6541 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6601 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6661 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6721 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6781 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6841 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6901 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6961 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 7021 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 7081 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 7141 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 7201 gtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???