pCMV-T7-Cfap206-3×FLAG-Neo plasmid

PVT105136

pCMV-T7-Cfap206-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-T7-Cfap206(mouse)-3×FLAG-Neo
Alias: NM_027041.4;Cfap206/1700003M02Rik/AU024269
Gene lenght: 1515
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-T7-Cfap206-3×FLAG-Neo plasmid Description

pCMV-T7-Cfap206-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-T7-Cfap206-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccATGCC TCCCACTCAA GCAGAAAGTG 781 TGATAAAGAA TATCATACGA GAAATAGGAC AAGAATGTGC AGCCCACGGA GAGATCGCTT 841 CAGAGACGGT GGTTGCTTTT ATGGTGAAAG CTGTCGTCCT AGATCCCAGC AATGGCTTTA 901 ACATGGATAG GACCCTCATC AAGACTGACG TGCAGAAACT TGTTAAGCTT TGTGTGGCAC 961 GGCTACTGGA CAATAAAAAT CCATCCCTGG ACACCATTAA GATGCAGGTC TACTTTGATA 1021 TGAACTATAC AAGTCGAGAG GACTTTCTTG AAGAACATCA CCGGGTCTTA GAATCCAGGT 1081 TAGGCATTGT TAGCAGAGAA ATCACAGACA ACAGAGCAAG TGCTAGGGAA GAGCTGGAAA 1141 ATCTCTACAG AAAGATCGTC AGCTACGTGC TCCTGCGCTC TGGGCTTGGA TCCCCCACAG 1201 ACATCAAGAT TGTCAGAGAG GCAACAGCCG CTCTGCAGAG CGTTTTCCCT CAGGCTGAGC 1261 TCGCCACGTT TCTGACCCTC AGTAAGAAGG ACAAAGAGCG CCAGTTGAAA GAGCTCACCA 1321 TGATTGTTAC CGGAATTCGC TTATTTAACA GAGACTGTGG AAAAGGAGGA GAAGGCATTG 1381 ATGATTTGCC GGCCATTCTC CACGAAGCGA TCCCAACCAC CACACAGCAC ATAGACTCCC 1441 AGCTTCAGAT TGCCCAGGAC CAGGCGTTTC GCTACACGGC CATCCTTGAG AAAGTAACCA 1501 ACAACCCCCT CATGGCCAAA GAACTCCAGC CGTATATGTT AAAGGAAGCA CTGTACAATG 1561 TTCGGCAGTA TGAGATCTTC CTGCAGACTG TTTTGTCAGA TATAATTACT TGTGCTGAGG 1621 AAGTAGAGAT GATGATAAAA CAATTAGCAG CCCAACTGGA ACAACTGAAA ATGGCAGTAA 1681 AATCTAAGAC GGCGGTCCCA ACATCCCAAG TCTTCCCCAT CTTCGTTGCG CTGGCGAGTC 1741 TGTGGATGAG CTTCCAGGAC GAGACGGTTC TGATCAGCGT TCTCAGTAAC TTGACCACCA 1801 ACCTGGAGCT CTTCCTGGGC ACCCACGAGC TCCTCTTTCC CGAGAAAGTG ATGCTAGGGC 1861 TTCTCGATGG CGTCGTCGTG AAAAGCGATT TGACGAGGAT AGAGGAACAC ATGGAAGAAA 1921 GAGTGGAGTT GGCAGATTTC AGAACACAGG AATGGCTTTT TCCAGAAACA ACAGCAAACT 1981 TTAACAAACT GTTAATTCAG TATCGTGGAT TTTGTGGCTA CACATTTGCT GTGACTGATG 2041 GCCTTCTCCT CCCAGGAAAT CCAGCCATTG GAATTTTGAA ATACAAAGAA AAATATTATA 2101 CTTTCAGTAC CAGAGATGCT GCATACACAT TCGCAGAAAA TCCAGACCAT TACATCCATT 2161 TGATTAAGGA GAAAGCCAAG AAGAATGCAG AGTTAATTCA GCTACTAGAA CTTCATCAGC 2221 AGTTTGAAAC TCTCATACCC TATTCTCAGG TAAACATGGC ATTCTCTGGA TCCGACTACA 2281 AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT 2341 AGTGAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 2401 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 2461 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 2521 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 2581 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 2641 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 2701 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 2761 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 2821 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 2881 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 2941 CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA 3001 GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT 3061 TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC 3121 CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG 3181 TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG 3241 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 3301 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 3361 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 3421 GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 3481 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 3541 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 3601 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA 3661 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA 3721 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 3781 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 3841 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 3901 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 3961 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 4021 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 4081 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 4141 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 4201 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 4261 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 4321 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 4381 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 4441 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 4501 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 4561 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 4621 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 4681 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 4741 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 4801 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 4861 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA 4921 AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT 4981 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG 5041 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG 5101 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 5161 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC 5221 TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 5281 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 5341 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 5401 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 5461 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 5521 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 5581 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 5641 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 5701 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 5761 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 5821 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 5881 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 5941 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 6001 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 6061 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 6121 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 6181 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 6241 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 6301 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 6361 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 6421 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 6481 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 6541 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 6601 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 6661 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 6721 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 6781 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 6841 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 6901 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 6961 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 7021 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 7081 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 7141 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 7201 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 7261 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 7321 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 7381 CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT 7441 CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG 7501 TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC 7561 GACAATTGCA TGAAGAATCT GCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???