pCMV-T7-Col5a2-3×FLAG-Neo plasmid

PVT102958

pCMV-T7-Col5a2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-T7-Col5a2(mouse)-3×FLAG-Neo
Alias: NM_007737.2;1110014L14Rik
Gene lenght: 4491
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-T7-Col5a2-3×FLAG-Neo plasmid Description

pCMV-T7-Col5a2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-T7-Col5a2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgat ggcaaactgg gtgggagcaa 781 gacctctcct cattctttct gttttattag ggtactgtgt ctcaataaaa gcccaggaac 841 aagagaatga tgaatatgat gaagaaatag cctgtactca acatggacag atgtatttaa 901 atagagacat ttggaaacct tctccatgtc agatctgtgt ctgtgacaat ggtgccattc 961 tttgtgacaa gatagagtgc ccagaggtgc tcaactgtgc caatcctata acacccactg 1021 gggagtgctg tccggtgtgc ccacaaactg gaggtggaga tactagtttt ggtagaggaa 1081 gaaagggaca aaaaggagaa ccaggattag tgcctgttgt aacaggtata cgcggtcgtc 1141 caggacctgc aggcccccca ggatcacaag gaccaagagg ggaccgaggc cctaaaggaa 1201 gacctggtcc tcgtggtcct cagggaattg atggagagcc aggtgtgcct ggtcagccag 1261 gtgctccagg gcctcctggg cacccctctc acccaggacc tgatggcatg agcaggcctt 1321 tttcagctca gatggctggg ctggatgaaa aatctggact tggaagtcaa gtaggactca 1381 tgcctggctc tgtgggtcct gttggcccaa gaggacctca gggtttacaa ggacagcaag 1441 gtggtgtggg acccgcagga cctcctggtg agcctggcga gcctggacca atgggtccaa 1501 ttggttctcg tggaccagag ggcccacctg ggaagcccgg agaagatggt gaacctggaa 1561 gaaatgggaa tacaggtgaa gtgggattct caggatcacc aggtgctcga ggcttccctg 1621 gtgctcctgg tctccctggc ttgaaaggtc acagaggaca caaaggtctt gaaggcccca 1681 aaggtgaaat tggagcacct ggtgctaagg gtgaagctgg ccccactggt ccaatgggtg 1741 ctatgggccc tctgggacca agaggaatgc caggagagag gggaagactt gggccacagg 1801 gtgctcctgg aaaacggggt gcccacggta tgcctggaaa acctggacca atgggtcctc 1861 ttgggatacc tggctcttcc ggttttccag gaaatcctgg aatgaaggga gaagcaggtc 1921 ctactggagc ccgaggccct gaaggtcctc aggggcagag aggtgaaact gggccacctg 1981 gtccagctgg ctctcagggt cttcctggtg cagtggggac tgatggtaca cctggcgcca 2041 aaggccccac aggctctgca ggaacctctg gccctcctgg cttggcaggg ccccctgggt 2101 ctcctggccc tcaaggcagt actggacctc agggaatccg aggacaatcg ggtgatccag 2161 gagttccagg tttcaaagga gaagctggtc caaagggaga accagggcca catggtattc 2221 agggtcccat tggtccacct ggtgaagaag gcaaaagagg tcctcggggt gatccaggca 2281 cagttggtcc tccaggacca atgggagaaa ggggcgctcc tggcaatcgt ggttttccag 2341 ggtctgatgg tttacctgga ccaaagggtg ctcagggaga gcgtggtcct gtaggttctt 2401 cggggcctaa aggaggccag ggagatccag gacgtccagg agaacctggg cttccaggtg 2461 ctcggggtct gacaggaaat cctggtgttc aaggtcctga aggaaaactt gggcctttgg 2521 gtgctcctgg ggaagatggt cggccaggtc ctccaggctc cattgggatc agaggacagc 2581 ctggaagcat gggccttcca ggccccaaag gtagcagtgg tgaccttgga aaaccaggag 2641 aagcaggaaa tgctggtgtg cctgggcaaa ggggggctcc tggcaaagat ggagaagttg 2701 gtccttcagg ccctgtggga cctccgggcc tagctgggga aagaggagag cagggacctc 2761 caggccccac tggctttcag ggacttcctg gacctccagg gccacctgga gaaggtggaa 2821 aggctggtga tcaaggtgtt cctggagagc ctggagcagt tggcccatta ggacctcggg 2881 gagaaagagg gaatcctgga gaaagaggag agccagggat aactggactg cctggggaga 2941 aaggcatggc tggaggacat ggccctgatg gtccaaaggg caacccaggt ccaactggga 3001 ccattggaga cacagggcca ccaggtcttc agggcatgcc aggagaaaga ggaattgcag 3061 gaacacctgg ccctaagggt gaccgaggtg gcataggaga gaaaggtgct gaaggaacag 3121 caggaaatga cggggcaagg ggtcttccag gtcccttggg acctccaggt cctgcaggcc 3181 ctactggaga aaagggtgaa cctggtccta gaggcttagt tggccctcct ggctcccgtg 3241 gcaatcctgg ttctcgtggt gaaaatggtc caactggagc tgttggtttt gctggacccc 3301 agggtcccga tggacagcct ggtgtcaagg gtgagcctgg agagccagga cagaagggag 3361 atgctgggtc tcctggaccc caagggctag caggatcacc tggcccccat ggtccacatg 3421 gtgttcctgg actgaaaggc ggtagaggaa cccagggtcc acctggtgct acagggtttc 3481 ctggttctgc tggcagagtt gggcctccag gccctgctgg agctccagga cctgcagggc 3541 ccgcaggaga acctgggaag gaaggacctc ctggtcttcg tggtgaccct ggctctcatg 3601 ggcgagtggg agatagagga ccagctggcc cacctggtag cccaggagat aaaggggacc 3661 caggagaaga tggacaacct ggtccagatg gtcctcctgg tcccgctggt accactgggc 3721 aaagaggaat tgttggaatg cccggacaac gtggggaacg aggaatgcct ggccttcctg 3781 gcccagcggg tacaccagga aaagtaggac caactggtgc gacaggagat aaaggtcccc 3841 ctggacctgt aggacctcca ggttccaatg gtcctgtagg agaacctggg ccagaaggtc 3901 cggctggtaa tgatggtacc ccaggacggg atggtgctgt tggagaacgg ggtgaccgtg 3961 gagaccccgg gcctgctggt ctaccaggct ctcagggtgc ccctggaact cctggccctg 4021 ttggtgcccc tggagatgca ggacaaagag gagagccagg ttctcgaggc cctgtaggac 4081 cacctggtcg agctggaaag cgtggtttac ctggaccaca aggacctcga ggtgacaagg 4141 gtgacaatgg agacagaggt gaccgaggtc agaaaggcca cagagggttc actggcctgc 4201 agggtcttcc tggacctcct ggtccaaatg gtgaacaagg aagtgctggc attcctggtc 4261 ctttcggtcc aagaggtcct ccaggaccag taggtccttc aggaaaggaa ggaaaccctg 4321 ggccacttgg accaattgga cctcctggtg tgcggggcag tgtaggagaa gcaggaccag 4381 agggtcctcc tggtgaacct gggcctcctg gccctccagg tccccctggc catcttactg 4441 ctgctcttgg tgatatcatg ggacactatg atgagaacat gccagatcca cttccagagt 4501 ttacggaaga ccaggcagct ccagatgaca caaacaaaac agaccctggg atccatgtta 4561 ccctgaagtc tctcagtagt cagattgaaa ccatgcgtag ccctgatggt tccaagaaac 4621 acccagcccg cacttgtgat gatttaaagc tttgccatcc cacaaagcag agcggtgaat 4681 attggattga tcctaaccag ggatcagctg aagatgcaat caaagtttac tgcaatatgg 4741 aaacaggaga gacatgtatt tcagcaaacc cagccagtgt tccacgtaaa acctggtggg 4801 ccagcaaatc ccctgacaat aagcctgtgt ggtacggtct tgacatgaat agaggatctc 4861 agttcacata tggagattac cagtctccta acacagctat cactcagatg acctttttgc 4921 gtcttttatc aaaagaagcc tcgcagaacc ttacttacat ctgtaggaac actgttggat 4981 atatggatga tcaagctaaa aacctcaaga aagctgtggt ccttaaaggg tcaaacgact 5041 tagaaataaa aggagaagga aacatcagat tcagatacac agttcttcaa gacacctgct 5101 ctaagcgaaa tggaaatgta ggcaaaacta tctttgaata cagaacacag aatgtggccc 5161 gcttgcccat catagatgtt ggtcctgtgg atattggcaa tgcagaccag gaatttggcc 5221 ttgatattgg gccagtttgt ttcatgGGAT CCGACTACAA AGACCATGAC GGTGATTATA 5281 AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GTGAGGGCCC GATATCTCGA 5341 CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 5401 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 5461 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 5521 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 5581 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 5641 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 5701 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT 5761 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 5821 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 5881 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG 5941 GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA 6001 ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG 6061 GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG 6121 CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC 6181 CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA 6241 TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT 6301 GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT 6361 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG 6421 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG 6481 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 6541 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 6601 TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 6661 ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 6721 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 6781 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 6841 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 6901 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC 6961 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 7021 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 7081 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 7141 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 7201 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 7261 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 7321 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 7381 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 7441 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 7501 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 7561 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 7621 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 7681 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG 7741 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 7801 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 7861 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC 7921 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG 7981 AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT 8041 CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT 8101 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 8161 CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC 8221 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 8281 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 8341 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 8401 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT 8461 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 8521 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 8581 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 8641 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 8701 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 8761 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 8821 ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 8881 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 8941 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 9001 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 9061 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 9121 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 9181 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 9241 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 9301 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 9361 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC 9421 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT 9481 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC 9541 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC 9601 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG 9661 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG 9721 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG 9781 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG 9841 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT 9901 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA 9961 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC 10021 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC 10081 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC 10141 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC 10201 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA 10261 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 10321 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT 10381 CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT 10441 GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC 10501 GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG 10561 CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???