(不稳??? pCMV-T7-Ncl-3×FLAG-Neo plasmid
PVT105272
(不稳??? pCMV-T7-Ncl-3×FLAG-Neo plasmid Information
Plasmid name: pCMV-T7-Ncl(rat)-3×FLAG-Neo
Alias: NM_012749.2
Gene lenght: 2142
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:
(不稳??? pCMV-T7-Ncl-3×FLAG-Neo plasmid Description
(不稳??? pCMV-T7-Ncl-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.
(不稳??? pCMV-T7-Ncl-3×FLAG-Neo plasmid Sequence
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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Recombinant DNA Molecular Cloning and Expression Vector Architecture
(重组DNA分子克隆与表达载体工程构建技术). This ensures maximum reproducibility across clinical research and scientific workflows.