pCMV-Trim24-3×FLAG-Neo plasmid

PVT110655

pCMV-Trim24-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Trim24(mouse)-3×FLAG-Neo
Alias: NM_145076.4;A130082H20Rik; D430004I05Rik; TIF1;TIF1-alpha; Tif1a; TIF1alpha
Gene lenght: 3153
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Trim24-3×FLAG-Neo plasmid Description

pCMV-Trim24-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Trim24-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga ggtggctgtg gagaaggcgg 781 cggcggcagc ggctccggcc ggaggccccg cagcggcggc gccgagcggg gagaatgagg 841 ccgagagccg gcagggcccg gactcggaga gcggcggcga ggcgtcccgg ctcaacctgt 901 tggacacttg cgccgtgtgc caccagaaca tccagagccg ggtgcccaag ctgctgccct 961 gcctgcactc gttctgccag cgctgtttgc ccgcgccgca gcgctatctc atgctgacgg 1021 cgcccgcgct gggctcggca gagacccctc cacccgctcc cgcccccgcc cccgccccgg 1081 gctccccggc cggtggtcct tcgccattcg ccacccaagt tggagtcatt cgatgcccag 1141 tttgcagtca agagtgtgct gagagacaca tcatagacaa cttttttgtg aaggacacca 1201 ctgaagttcc tagtagtaca gtagaaaagt ctaatcaggt atgtacaagc tgtgaagaca 1261 atgcagaagc taatgggttt tgtgtagagt gtgttgaatg gctctgcaag acatgtatta 1321 gagctcacca gagggtgaag ttcacaaaag accacacagt caggcagaaa gaagaagtat 1381 ctccagaggc agttggggtg accagtcagc gaccagtgtt ttgtcccttc cataaaaagg 1441 agcagttgaa actttactgt gaaacatgtg ataaactgac ctgtcgagac tgccagctgc 1501 tagaacacaa agaacacagg tatcaattta tagaagaagc ttttcagaat caaaaagtga 1561 tcatagatac tctaatcacc aaactgatgg aaaaaacaaa atatataaag tatacaggaa 1621 atcagatcca aaataggata attgaaataa atcaaaacca aaagcaggtg gaacaggata 1681 ttaaagttgc catcttcaca ttgatggtgg agataaacaa aaaagggaaa gctctgctgc 1741 accagcttga gagtcttgca aaggaccatc gaatgaaact catgcaacaa cagcaggaag 1801 tggctgggct ttctaagcag ttagagcacg tcatgcattt ttctaaatgg gctgtttcca 1861 gtggcagcag cacagccttg ctgtacagca agcggctgat tacatacagg ttacggcacc 1921 ttcttcgtgc aaggtgtgat gcttctcctg tgaccaacac caccatccag tttcactgtg 1981 atcctagttt ctgggctcaa aatattatca acttgggttc tttagtaatc gaggataaag 2041 agagccagcc acaaatgcct aagcagaatc ctgtcgtgga gcagagttca cagccaccag 2101 gtggtttacc ttccaaccag ttatccaagt tcccaacaca gatcagccta gctcagttac 2161 gactccagca tattcagcaa caggtaatgg ctcagaggca acaggtgcaa cggaggccag 2221 cacctgtggg tttaccaaac cctagaatgc aggggcccat ccagcagcct tccatctctc 2281 atcagcatcc gccaccacgc ttaataaact ttcagaatca cagccctaag cccaatggac 2341 cagttcttcc tccttatcct cagcagctga gatattcacc aagccagaat gtacctcggc 2401 agacaacaat aaagcccaac cccttgcaaa tggctttttt ggctcaacag gccataaaac 2461 agtggcagat cagcagtgta caggctccgc ccacaactgc cagcagctcc tcctccacgc 2521 cgtccagccc cacaatcaca agtgcagctg ggtacgatgg aaaagctttt agttcaccca 2581 tgattgatct gagtgcaccg gtgggagggt cttacaatct tccttctctt ccagatattg 2641 attgttcaag tactataatg ttggacaaca ttgcaaggaa agacacaggt gtagatcacg 2701 cccagccgag gcctccgtca aacagaacgg tgcagtcacc aaattcatca gtgccatctc 2761 caggccttgc agggcctgtt actatgacta gcgtccatcc cccaatacgt tcacctagtg 2821 cctccagtgt tggaagtcga ggaagctctg gctcttccag caaaccagca ggagctgatt 2881 ctactcacaa ggtcccagta gtcatgctgg agccaattcg aataaaacag gaaaacagtg 2941 gaccacctga aaattatgat tttcctgttg ttatagtaaa acaagaatca gatgaagaat 3001 ctagacctca aaatactaac tatccaagaa gcatacttac ctccctcctc ttaaacagca 3061 gtcagagctc tgcttctgag gaaaccgtgt tacgatctga tgcccctgat agtacaggag 3121 atcagcctgg actccatcaa gaaaattcct caaatggaaa gtctgagtgg tcggatgcct 3181 cccagaagtc ccctgtgcat gtcggagaga cgaggaagga ggatgacccc aatgaagact 3241 ggtgtgctgt ttgtcaaaat ggtggggaac tcctatgctg tgagaaatgt cctaaagtat 3301 tccatcttac ttgtcatgtg cccaccttga caaattttcc aagtggagaa tggatctgta 3361 ctttctgccg agacttatct aagccagagg ttgactatga ttgtgatgtt cccagtcacc 3421 actcagagaa acggaaaagt gaaggcctta ctaagttaac gccaatagac aaaaggaaat 3481 gtgaacgcct acttctgttt ctttactgcc atgaaatgag cctggctttc caagaccctg 3541 ttcctctaac tgtgcctgat tattataaaa taattaaaaa cccaatggac ttgtcaacca 3601 tcaagaaaag acttcaggag gattattgca tgtatacaaa gcctgaagac tttgtagctg 3661 attttagatt gatctttcaa aactgtgctg aattcaatga gcctgattct gaagtagcca 3721 atgctggtat aaaacttgaa agctattttg aagaacttct aaagaatctt tatccagaaa 3781 aaaggtttcc taaggtagaa ttcaggcatg aagcagaaga ctgtaagttc agtgacgact 3841 cagacgatga ctttgtacag ccccggaaga agcgtctcaa gagcaccgag gatcgccagc 3901 tgcttaagGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT 3961 ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGATATCTC GACAATCAAC CTCTGGATTA 4021 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 4081 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 4141 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 4201 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 4261 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 4321 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 4381 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG 4441 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 4501 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 4561 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGAAAC GGGGGAGGCT AACTGAAACA 4621 CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA 4681 ACGCACGGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC 4741 TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC 4801 ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG 4861 CCCTGCCATA GCAGATCTGC GCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG 4921 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 4981 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 5041 TCCCCGTCAA GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 5101 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 5161 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 5221 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG 5281 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT 5341 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5401 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 5461 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 5521 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 5581 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG 5641 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 5701 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 5761 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 5821 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 5881 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 5941 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 6001 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 6061 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 6121 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 6181 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 6241 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 6301 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 6361 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 6421 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 6481 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 6541 GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA 6601 TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC 6661 CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT 6721 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA 6781 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA 6841 TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 6901 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 6961 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 7021 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 7081 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 7141 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 7201 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 7261 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 7321 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 7381 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 7441 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA 7501 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 7561 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 7621 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 7681 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 7741 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 7801 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 7861 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 7921 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 7981 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 8041 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 8101 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 8161 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 8221 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 8281 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8341 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8401 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8461 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8521 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8581 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8641 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8701 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 8761 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 8821 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 8881 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 8941 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 9001 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 9061 CCCGATCCCC TATGGTCGAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG 9121 TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT GCGCGAGCAA AATTTAAGCT 9181 ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.