pCMV-TSC2-8×His-Neo plasmid

PVT101498

pCMV-TSC2-8×His-Neo plasmid Information

Plasmid name: pCMV-TSC2(human)-8×His-Neo
Alias: NM_001406676.1;LAM; PPP1R160; TSC4
Gene lenght: 5202bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TSC2-8×His-Neo plasmid Description

pCMV-TSC2-8×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TSC2-8×His-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga atgtggcctc aacaatcgca 781 tccggatgat agggcagatt tgtgaagtcg caaaaaccaa gaaatttgaa gagcacgcag 841 tggaagcact ctggaaggcg gtcgcggatc tgttgcagcc ggagcggccg ctggaggccc 901 ggcacgcggt gctggctctg ctgaaggcca tcgtgcaggg gcagggcgag cgtttggggg 961 tcctcagagc cctcttcttt aaggtcatca aggattaccc ttccaacgaa gaccttcacg 1021 aaaggctgga ggttttcaag gccctcacag acaatgggag acacatcacc tacttggagg 1081 aagagctggc tgactttgtc ctgcagtgga tggatgttgg cttgtcctcg gaattccttc 1141 tggtgctggt gaacttggtc aaattcaata gctgttacct cgacgagtac atcgcaagga 1201 tggttcagat gatctgtctg ctgtgcgtcc ggaccgcgtc ctctgtggac atagaggtct 1261 ccctgcaggt gctggacgcc gtggtctgct acaactgcct gccggctgag agcctcccgc 1321 tgttcatcgt taccctctgt cgcaccatca acgtcaagga gctctgcgag ccttgctgga 1381 agctgatgcg gaacctcctt ggcacccacc tgggccacag cgccatctac aacatgtgcc 1441 acctcatgga ggacagagcc tacatggagg acgcgcccct gctgagagga gccgtgtttt 1501 ttgtgggcat ggctctctgg ggagcccacc ggctctattc tctcaggaac tcgccgacat 1561 ctgtgttgcc atcattttac caggccatgg catgtccgaa cgaggtggtg tcctatgaga 1621 tcgtcctgtc catcaccagg ctcatcaaga agtataggaa ggagctccag gtggtggcgt 1681 gggacattct gctgaacatc atcgaacggc tccttcagca gctccagacc ttggacagcc 1741 cggagctcag gaccatcgtc catgacctgt tgaccacggt ggaggagctg tgtgaccaga 1801 acgagttcca cgggtctcag gagagatact ttgaactggt ggagagatgt gcggaccaga 1861 ggcctgagtc ctccctcctg aacctgatct cctatagagc gcagtccatc cacccggcca 1921 aggacggctg gattcagaac ctgcaggcgc tgatggagag attcttcagg agcgagtccc 1981 gaggcgccgt gcgcatcaag gtgctggacg tgctgtcctt tgtgctgctc atcaacaggc 2041 agttctatga ggaggagctg attaactcag tggtcatctc gcagctctcc cacatccccg 2101 aggataaaga ccaccaggtc cgaaagctgg ccacccagtt gctggtggac ctggcagagg 2161 gctgccacac acaccacttc aacagcctgc tggacatcat cgagaaggtg atggcccgct 2221 ccctctcccc acccccggag ctggaagaaa gggatgtggc cgcatactcg gcctccttgg 2281 aggatgtgaa gacagccgtc ctggggcttc tggtcatcct tcagaccaag ctgtacaccc 2341 tgcctgcaag ccacgccacg cgtgtgtatg agatgctggt cagccacatt cagctccact 2401 acaagcacag ctacaccctg ccaatcgcga gcagcatccg gctgcaggcc tttgacttcc 2461 tgttgctgct gcgggccgac tcactgcacc gcctgggcct gcccaacaag gatggagtcg 2521 tgcggttcag cccctactgc gtctgcgact acatggagcc agagagaggc tctgagaaga 2581 agaccagcgg ccccctttct cctcccacag ggcctcctgg cccggcgcct gcaggccccg 2641 ccgtgcggct ggggtccgtg ccctactccc tgctcttccg cgtcctgctg cagtgcttga 2701 agcaggagtc tgactggaag gtgctgaagc tggttctggg caggctgcct gagtccctgc 2761 gctataaagt gctcatcttt acttcccctt gcagtgtgga ccagctgtgc tctgctctct 2821 gctccatgct ttcaggccca aagacactgg agcggctccg aggcgcccca gaaggcttct 2881 ccagaactga cttgcacctg gccgtggttc cagtgctgac agcattaatc tcttaccata 2941 actacctgga caaaaccaaa cagcgcgaga tggtctactg cctggagcag ggcctcatcc 3001 accgctgtgc cagccagtgc gtcgtggcct tgtccatctg cagcgtggag atgcctgaca 3061 tcatcatcaa ggcgctgcct gttctggtgg tgaagctcac gcacatctca gccacagcca 3121 gcatggccgt cccactgctg gagttcctgt ccactctggc caggctgccg cacctctaca 3181 ggaactttgc cgcggagcag tatgccagtg tgttcgccat ctccctgccg tacaccaacc 3241 cctccaagtt taatcagtac atcgtgtgtc tggcccatca cgtcatagcc atgtggttca 3301 tcaggtgccg cctgcccttc cggaaggatt ttgtcccttt catcactaag ggcctgcggt 3361 ccaatgtcct cttgtctttt gatgacaccc ccgagaagga cagcttcagg gcccggagta 3421 ctagtctcaa cgagagaccc aagagtctga ggatagccag accccccaaa caaggcttga 3481 ataactctcc acccgtgaaa gaattcaagg agagctctgc agccgaggcc ttccggtgcc 3541 gcagcatcag tgtgtctgaa catgtggtcc gcaggataca gacgtccctc accagtgcca 3601 gcttggggtc tgcagatgag aactccgtgg cccaggctga cgatagcctg aaaaacctcc 3661 acctggagct cacggaaacc tgtctggaca tgatggctcg atacgtcttc tccaacttca 3721 cggctgtccc gaagaggtct cctgtgggcg agttcctcct agcgggtggc aggaccaaaa 3781 cctggctggt tgggaacaag cttgtcactg tgacgacaag cgtgggaacc gggacccggt 3841 cgttactagg cctggactcg ggggagctgc agtccggccc ggagtcgagc tccagccccg 3901 gggtgcatgt gagacagacc aaggaggcgc cggccaagct ggagtcccag gctgggcagc 3961 aggtgtcccg tggggcccgg gatcgggtcc gttccatgtc ggggggccat ggtcttcgag 4021 ttggcgccct ggacgtgccg gcctcccagt tcctgggcag tgccacttct ccaggaccac 4081 ggactgcacc agccgcgaaa cctgagaagg cctcagctgg cacccgggtt cctgtgcagg 4141 agaagacgaa cctggcggcc tatgtgcccc tgctgaccca gggctgggcg gagatcctgg 4201 tccggaggcc cacagggaac accagctggc tgatgagcct ggagaacccg ctcagccctt 4261 tctcctcgga catcaacaac atgcccctgc aggagctgtc taacgccctc atggcggctg 4321 agcgcttcaa ggagcaccgg gacacagccc tgtacaagtc actgtcggtg ccggcagcca 4381 gcacggccaa accccctcct ctgcctcgct ccaacacaga ctccgccgtg gtcatggagg 4441 agggaagtcc gggcgaggtt cctgtgctgg tggagccccc agggttggag gacgttgagg 4501 cagcgctagg catggacagg cgcacggatg cctacagcag gtcgtcctca gtctccagcc 4561 aggaggagaa gtcgctccac gcggaggagc tggttggcag gggcatcccc atcgagcgag 4621 tcgtctcctc ggagggtggc cggccctctg tggacctctc cttccagccc tcgcagcccc 4681 tgagcaagtc cagctcctct cccgagctgc agactctgca ggacatcctc ggggaccctg 4741 gggacaaggc cgacgtgggc cggctgagcc ctgaggttaa ggcccggtca cagtcaggga 4801 ccctggacgg ggaaagtgct gcctggtcgg cctcgggcga agacagtcgg ggccagcccg 4861 agggtccctt gccttccagc tccccccgct cgcccagtgg cctccggccc cgaggttaca 4921 ccatctccga ctcggcccca tcacgcaggg gcaagagagt agagagggac gccttaaaga 4981 gcagagccac agcctccaat gcagagaaag tgccaggcat caaccccagt ttcgtgttcc 5041 tgcagctcta ccattccccc ttctttggcg acgagtcaaa caagccaatc ctgctgccca 5101 atgagtcaca gtcctttgag cggtcggtgc agctcctcga ccagatccca tcatacgaca 5161 cccacaagat cgccgtcctg tatgttggag aaggccagag caacagcgag ctcgccatcc 5221 tgtccaatga gcatggctcc tacaggtaca cggagttcct gacgggcctg ggccggctca 5281 tcgagctgaa ggactgccag ccggacaagg tgtacctggg aggcctggac gtgtgtggtg 5341 aggacggcca gttcacctac tgctggcacg atgacatcat gcaagccgtc ttccacatcg 5401 ccaccctgat gcccaccaag gacgtggaca agcaccgctg cgacaagaag cgccacctgg 5461 gcaacgactt tgtgtccatt gtctacaatg actccggtga ggacttcaag cttggcacca 5521 tcaagggcca gttcaacttt gtccacgtga tcgtcacccc gctggactac gagtgcaacc 5581 tggtgtccct gcagtgcagg aaagacatgg agggccttgt ggacaccagc gtggccaaga 5641 tcgtgtctga ccgcaacctg cccttcgtgg cccgccagat ggccctgcac gcaaatatgg 5701 cctcacaggt gcatcatagc cgctccaacc ccaccgatat ctacccctcc aagtggattg 5761 cccggctccg ccacatcaag cggctccgcc agcggatctg cgaggaagcc gcctactcca 5821 accccagcct acctctggtg caccctccgt cccatagcaa agcccctgca cagactccag 5881 ccgagcccac acctggctat gaggtgggcc agcggaagcg cctcatctcc tcggtggagg 5941 acttcaccga gtttgtgGGA TCCCATCACC ACCATCACCA TCATCACTAG TGAGGGCCCG 6001 ATATCTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 6061 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT 6121 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA 6181 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC 6241 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT 6301 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG 6361 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC 6421 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC 6481 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT 6541 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC 6601 TGGAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT 6661 ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG 6721 CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA 6781 ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG 6841 GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC 6901 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 6961 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 7021 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT 7081 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 7141 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 7201 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 7261 TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 7321 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 7381 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 7441 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 7501 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 7561 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 7621 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 7681 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 7741 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 7801 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 7861 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 7921 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 7981 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 8041 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 8101 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 8161 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 8221 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 8281 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 8341 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 8401 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 8461 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 8521 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGCGAAA TGACCGACCA 8581 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 8641 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 8701 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 8761 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 8821 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 8881 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 8941 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 9001 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 9061 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 9121 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 9181 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 9241 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 9301 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 9361 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 9421 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 9481 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 9541 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 9601 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 9661 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 9721 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 9781 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 9841 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 9901 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 9961 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 10021 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 10081 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 10141 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 10201 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 10261 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 10321 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 10381 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 10441 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 10501 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 10561 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 10621 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 10681 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 10741 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 10801 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 10861 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 10921 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 10981 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 11041 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC 11101 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT 11161 GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG 11221 AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???