pCMV-XPO1-6×His-Neo plasmid

PVT103912

pCMV-XPO1-6×His-Neo plasmid Information

Plasmid name: pCMV-XPO1(human)-6×His-Neo
Alias: NM_003400.4;CRM-1; CRM1; emb; exp1;
Gene lenght: 3213
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-XPO1-6×His-Neo plasmid Description

pCMV-XPO1-6×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-XPO1-6×His-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 Cgccaccatg ccagcaatta tgacaatgtt agcagaccat gcagctcgtc agctgcttga 961 tttcagccaa aaactggata tcaacttatt agataatgtg gtgaattgct tataccatgg 1021 agaaggagcc cagcaaagaa tggctcaaga agtactgaca catttaaagg agcatcctga 1081 tgcttggaca agagtcgaca caattttgga attttctcag aatatgaata cgaaatacta 1141 tggactacaa attttggaaa atgtgataaa aacaaggtgg aagattcttc caaggaacca 1201 gtgcgaagga ataaaaaaat acgttgttgg cctcattatc aagacgtcat ctgacccaac 1261 ttgtgtagag aaagaaaagg tgtatatcgg aaaattaaat atgatccttg ttcagatact 1321 gaaacaagaa tggcccaaac attggccaac ttttatcagt gatattgttg gagcaagtag 1381 gaccagcgaa agtctctgtc aaaataatat ggtgattctt aaactcttga gtgaagaagt 1441 atttgatttc tctagtggac agataaccca agtcaaatct aagcatttaa aagacagcat 1501 gtgcaatgaa ttctcacaga tatttcaact gtgtcagttt gtaatggaaa attctcaaaa 1561 tgctccactt gtacatgcaa ccttggaaac attgctcaga tttctgaact ggattcccct 1621 gggatatatt tttgagacca aattaatcag cacattgatt tataagttcc tgaatgttcc 1681 aatgtttcga aatgtctctc tgaagtgcct cactgagatt gctggtgtga gtgtaagcca 1741 atatgaagaa caatttgtaa cactatttac tctgacaatg atgcaactaa agcagatgct 1801 tcctttaaat accaatattc gacttgcgta ctcaaatgga aaagatgatg aacagaactt 1861 cattcaaaat ctcagtttgt ttctctgcac ctttcttaag gaacatgatc aacttataga 1921 aaaaagatta aatctcaggg aaactcttat ggaggccctt cattatatgt tgttggtatc 1981 tgaagtagaa gaaactgaaa tctttaaaat ttgtcttgaa tactggaatc atttggctgc 2041 tgaactctat agagagagtc cattctctac atctgcctct ccgttgcttt ctggaagtca 2101 acattttgat gttcctccca ggagacagct atatttgccc atgttattca aggtccgttt 2161 attaatggtt agtcgaatgg ctaaaccaga ggaagtattg gttgtagaga atgatcaagg 2221 agaagttgtg agagaattca tgaaggatac agattccata aatttgtata agaatatgag 2281 ggaaacattg gtttatctta ctcatctgga ttatgtagat acagaaagaa taatgacaga 2341 gaagcttcac aatcaagtga atggtacaga gtggtcatgg aaaaatttga atacattgtg 2401 ttgggcaata ggctccatta gtggagcaat gcatgaagag gacgaaaaac gatttcttgt 2461 tactgttata aaggatctat taggattatg tgaacagaaa agaggcaaag ataataaagc 2521 tattattgca tcaaatatca tgtacatagt aggtcaatac ccacgttttt tgagagctca 2581 ctggaaattt ctgaagactg tagttaacaa gctgttcgaa ttcatgcatg agacccatga 2641 tggagtccag gatatggctt gtgatacttt cattaaaata gcccaaaaat gccgcaggca 2701 tttcgttcag gttcaggttg gagaagtgat gccatttatt gatgaaattt tgaacaacat 2761 taacactatt atttgtgatc ttcagcctca acaggttcat acgttttatg aagctgtggg 2821 gtacatgatt ggtgcacaaa cagatcaaac agtacaagaa cacttgatag aaaagtacat 2881 gttactccct aatcaagtgt gggatagtat aatccagcag gcaaccaaaa atgtggatat 2941 actgaaagat cctgaaacag tcaagcagct tggtagcatt ttgaaaacaa atgtgagagc 3001 ctgcaaagct gttggacacc cctttgtaat tcagcttgga agaatttatt tagatatgct 3061 taatgtatac aagtgcctca gtgaaaatat ttctgcagct atccaagcta atggtgaaat 3121 ggttacaaag caaccattga ttagaagtat gcgaactgta aaaagggaaa ctttaaagtt 3181 aatatctggt tgggtgagcc gatccaatga tccacagatg gtcgctgaaa attttgttcc 3241 ccctctgttg gatgcagttc tcattgatta tcagagaaat gtcccagctg ctagagaacc 3301 agaagtgctt agtactatgg ccataattgt caacaagtta gggggacata taacagctga 3361 aatacctcaa atatttgatg ctgtttttga atgcacattg aatatgataa ataaggactt 3421 tgaagaatat cctgaacata gaacgaactt tttcttacta cttcaggctg tcaattctca 3481 ttgtttccca gcattccttg ctattccacc tacacagttt aaacttgttt tggattccat 3541 catttgggct ttcaaacata ctatgaggaa tgtcgcagat acgggcttac agatactttt 3601 tacactctta caaaatgttg cacaagaaga agctgcagct cagagttttt atcaaactta 3661 tttttgtgat attctccagc atatcttttc tgttgtgaca gacacttcac atactgctgg 3721 tttaacaatg catgcatcaa ttcttgcata tatgtttaat ttggttgaag aaggaaaaat 3781 aagtacatca ttaaatcctg gaaatccagt taacaaccaa atctttcttc aggaatatgt 3841 ggctaatctc cttaagtcgg ccttccctca cctacaagat gctcaagtaa agctctttgt 3901 gacagggctt ttcagcttaa atcaagatat tcctgctttc aaggaacatt taagagattt 3961 cctagttcaa ataaaggaat ttgcaggtga agacacttct gatttgtttt tggaagagag 4021 agaaatagcc ctacggcagg ctgatgaaga gaaacataaa cgtcaaatgt ctgtccctgg 4081 catctttaat ccacatgaga ttccagaaga aatgtgtgat GCGGCCGCAC ATCACCATCA 4141 TCACCATTGA CTCGAGTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC 4201 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 4261 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 4321 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 4381 CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG 4441 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 4501 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 4561 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 4621 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 4681 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 4741 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 4801 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 4861 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG 4921 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 4981 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 5041 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 5101 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 5161 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 5221 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 5281 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 5341 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 5401 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 5461 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 5521 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 5581 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 5641 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 5701 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 5761 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 5821 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 5881 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 5941 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 6001 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 6061 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 6121 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 6181 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 6241 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 6301 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 6361 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 6421 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 6481 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 6541 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 6601 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 6661 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 6721 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 6781 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 6841 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 6901 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 6961 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 7021 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 7081 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 7141 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 7201 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 7261 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 7321 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 7381 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 7441 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 7501 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 7561 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 7621 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 7681 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 7741 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 7801 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 7861 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 7921 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 7981 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 8041 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 8101 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 8161 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 8221 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 8281 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 8341 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 8401 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 8461 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 8521 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 8581 TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???