pCMV-Zbtb11-3×HA-IRES2-EGFP-Neo plasmid

PVT93523

pCMV-Zbtb11-3×HA-IRES2-EGFP-Neo plasmid Information

Plasmid name: pCMV-Zbtb11(mouse)-3×HA-IRES2-EGFP-Neo
Alias: NM_173026.2;9230110G02Rik; ZNF-U69274
Gene lenght: 3150bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: PIRES2-EGFP.P3
Copy number: High
Induction:

pCMV-Zbtb11-3×HA-IRES2-EGFP-Neo plasmid Description

pCMV-Zbtb11-3×HA-IRES2-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Zbtb11-3×HA-IRES2-EGFP-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atgtcaagcg aggagagcta ccgggccatc 961 ctgcgctacc tgacgaacga acgcgagccc tatgcgccgg gcaccgaggg caatgtcaag 1021 cggaagatcc gcaaggccgc cgcctgctac gtcgtgcgcg gcgggaccct ctactaccag 1081 cggcggcagc ggcaccgcaa gaccttcgcc gagctggagg tggtgttgca gccggagcgt 1141 cgccagggcc tcatcgaggc ggcccatttg ggccccgggg gcacccacca cacccggcac 1201 cagacctggc acgacctgtc caagacctac tggtggcgag gtatattaaa gcaagttaaa 1261 gattacataa aacagtgtag caaatgtcag gagaagctcg atcgctcccg tccaatatcg 1321 gatgcttcgg aaatgttgga agagttgggg ttggatcttg actctggaga agaaagtaat 1381 gagtcggagg atgacctgag caacttcacc tcccctccat ccacagcctc caagtcttcc 1441 aaaaagaagc cagtgtcgaa acatgagctg gtgtttgttg acaccaaagg agtggtcaaa 1501 cggtcctctc caaagcattg ccaggcggtt ctgaaacagt tgaatgagca gaggctttcc 1561 aaccagttct gtgacgtcac attgctcatc gagggggagg agtacaaagc gcacaagtct 1621 gtcctctcgg ctaatagtga atattttcga gatcttttta ttgaaaaagg agcagtttct 1681 agtcacgagg ctgttgtgga tctttctggt ttctgtaaag caagcttcct tcctttgctg 1741 gaatttgcct atacttctgt actaagtttt gacttttgta gcatggctga tgtagccgtc 1801 ttagctcgcc acctttttat gtcagaagtt ttagaaattt gtgaaagtgt acataaacta 1861 atggaagaaa aacagcttac agtgtataag aaaggagaag ttcaaacggt tgcatctact 1921 caggacttag cagcacacaa tggaactaca acaccacctg gcactaggaa tgaagcaacc 1981 acaactttgt ccggtgaact tgggcattgt gaaattgtac tgctggtaaa tggagaattg 2041 ccagaggcag agcagaatgg ggagccagag cagcagcctg cacctcaggc ttctccagag 2101 gctgaagcta gtgtgtcccc tgtagaaggt atacctgagc cccaccctga aatgggaact 2161 gcaagcttag caaaagaaag caaccaacca gagtccgcag tcaccagaga agatggcata 2221 gttgctagtg ttcatcctaa aatctcaaaa gagaatgtta ccaacgcctc ccaagaggac 2281 agtgatacag gaaacgatac atcacctgag gacatcggtg ccaaagactg tccagatcat 2341 agtcagagcc ctggccaacc ttcaaaggat gaggacactc taactgaagc tacagaaaag 2401 acagactcag gcccagatga tgatacttat aggagcaggc ttaggcagcg gtctgttaat 2461 gaagggggct atatccggct acacaagggc atggagaaaa agttacagaa gcggaaagcc 2521 atctctaagt cagcagttca acaggtggcc cagaaattag ttcaaagagg aaaaaagatg 2581 aaacaaccaa aacgggatgc taaggagagc accgaagaaa cggctcataa atgtggtgaa 2641 tgtggaatgg tttttccgag acgatatgcc tttataatgc acacattgaa acatgaaaga 2701 gctagagatt acaaatgtcc gttatgtaag aaacagtttc agtatagtgc ctcattacga 2761 gcacacctta ttcgacatac aagaaaagag gcacccactt catcctcatc caattccaca 2821 tctactgagg cgtcgggagg atcatctgag aagggaagaa ccaaaagaga atttatatgt 2881 tctatatgtg gaaggacatt acctaaatta tactctctcc gcatacatat gctaaagcac 2941 acgggtgtaa agccgcatgc atgccaggtg tgtggaaaaa cttttatcta taagcatggt 3001 ctaaaattgc atcaaagtct gcatcaatca caaaagcagt ttcagtgtga actgtgtgtc 3061 aagtcatttg ttaccaagcg gagtcttcag gaacatatga gtattcacac aggagagtcc 3121 aagtacttct gctcaatttg tggaaagtct tttcataggg gctctggact tagcaagcac 3181 cttaagaaac accagccaaa gcctgaagtt cgaggctatc attgtactca atgtgaaaaa 3241 agtttttttg aagccagaga tctacgccag catatgaaca aacatcttgg tgtgaagcca 3301 ttccagtgcc agttttgtga taagtgttac agctggaaga aagattggta ttcccatgtg 3361 aagtcccatt ctgtcactga gccttacagg tgtaatatat gcggcaaaga attttatgaa 3421 aaagctttat tcagaagaca tgtaaagaag gccacccatg ggaagaaagg aagagcaaag 3481 caaaacctgg agcgggtgtg tgatcagtgt gggaggaagt tcacgcagct gagagagtac 3541 aggagacaca tgaacaacca tgaaggcgtt aagcctttcg agtgcttaac atgtggagta 3601 gcgtgggctg atgcccgatc actaaagcgt catgtccgaa cacatactgg tgaacggccc 3661 tatgtgtgtc ctgtgtgtag tgaagcctac attgatgctc gaacacttcg taagcatatg 3721 actaaattcc acagagacta tgtgccttgc aaaattatgc tggaaaaaga cactctgcag 3781 tttcataacc aaggaacaca agtggaacat gctgtcagca tcttaactgc agacatgcag 3841 gaacaagaaa gcagtgggcc tcaagaactg gagactgtgg tggtgacagg agaaactatg 3901 gaagttcttg aagctgtggc agctactgag gagtgtccgt cggtgtctac cctctctgac 3961 caaagcatta tgcaagtggt taactatgtg ctggcgcagc agcaagggca gaagctgtct 4021 gaagttgcag aagccattca gactgttgaa gtggaggttg cacatatgcc agaggcagag 4081 TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC 4141 TATCCTTATG ATGTCCCAGA CTACGCATAG CTCGAGtaac tgcagtcgac ggtaccgcgg 4201 gcccgggatc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 4261 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 4321 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 4381 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 4441 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 4501 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 4561 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 4621 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 4681 ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc 4741 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca 4801 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 4861 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 4921 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 4981 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 5041 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 5101 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 5161 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 5221 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 5281 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 5341 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 5401 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 5461 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag 5521 gTCTAGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC 5581 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 5641 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 5701 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 5761 TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG 5821 GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 5881 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 5941 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 6001 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 6061 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 6121 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 6181 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 6241 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 6301 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 6361 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 6421 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 6481 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 6541 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 6601 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 6661 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 6721 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 6781 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 6841 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 6901 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 6961 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 7021 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 7081 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 7141 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 7201 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 7261 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 7321 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 7381 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 7441 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 7501 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 7561 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 7621 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 7681 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 7741 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 7801 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 7861 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 7921 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 7981 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 8041 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 8101 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 8161 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 8221 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 8281 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 8341 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 8401 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 8461 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 8521 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 8581 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 8641 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 8701 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 8761 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 8821 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 8881 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 8941 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 9001 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 9061 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 9121 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 9181 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 9241 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 9301 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 9361 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 9421 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 9481 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 9541 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 9601 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 9661 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 9721 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 9781 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 9841 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 9901 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 9961 ACGGATCGGG AGATCTCCCG ATCCCCTATG GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???