Catalog No. PVT11172
Packing 2ug
Function plant expression plasmid
Resistance Kan
Screen Hyg
Strain DB3.1
Culture temperature 37degrees centigrade
LOCUS Exported 12008 bp ds-DNA circular SYN 30-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12008)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..12008
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 115..367
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(439..744)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
promoter complement(1101..3091)
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
misc_feature 3332..3356
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4655..5284
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5718..6785
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 6851..7045
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 7389..7529
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7715..8303)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8390..9184)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 9609..9633
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 9711..9885
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(9925..10950)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(11017..11694)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
ORIGIN
1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
361 aacgatcggg gaaattcgga tccccaatac ttgtatgggt cgacctgcag actggctgtg
421 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
481 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
541 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
601 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
661 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
721 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
781 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
841 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
901 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
961 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
1021 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccccataca
1081 agtattgggg atcccccggg ctgcagaagt aacaccaaac aacagggtga gcatcgacaa
1141 aagaaacagt accaagcaaa taaatagcgt atgaaggcag ggctaaaaaa atccacatat
1201 agctgctgca tatgccatca tccaagtata tcaagatcaa aataattata aaacatactt
1261 gtttattata atagataggt actcaaggtt agagcatatg aatagatgct gcatatgcca
1321 tcatgtatat gcatcagtaa aacccacatc aacatgtata cctatcctag atcgatattt
1381 ccatccatct taaactcgta actatgaaga tgtatgacac acacatacag ttccaaaatt
1441 aataaataca ccaggtagtt tgaaacagta ttctactccg atctagaacg aatgaacgac
1501 cgcccaacca caccacatca tcacaaccaa gcgaacaaaa agcatctctg tatatgcatc
1561 agtaaaaccc gcatcaacat gtatacctat cctagatcga tatttccatc catcatcttc
1621 aattcgtaac tatgaatatg tatggcacac acatacagat ccaaaattaa taaatccacc
1681 aggtagtttg aaacagaatt ctactccgat ctagaacgac cgcccaacca gaccacatca
1741 tcacaaccaa gacaaaaaaa agcatgaaaa gatgacccga caaacaagtg cacggcatat
1801 attgaaataa aggaaaaggg caaaccaaac cctatgcaac gaaacaaaaa aaatcatgaa
1861 atcgatcccg tctgcggaac ggctagagcc atcccaggat tccccaaaga gaaacactgg
1921 caagttagca atcagaacgt gtctgacgta caggtcgcat ccgtgtacga acgctagcag
1981 cacggatcta acacaaacac ggatctaaca caaacatgaa cagaagtaga actaccgggc
2041 cctaaccatg gaccggaacg ccgatctaga gaaggtagag aggggggggg ggggaggacg
2101 agcggcgtac cttgaagcgg aggtgccgac gggtggattt gggggagatc tggttgtgtg
2161 tgtgtgcgct ccgaacaaca cgaggttggg gaaagagggt gtggaggggg tgtctattta
2221 ttacggcggg cgaggaaggg aaagcgaagg agcggtggga aaggaatccc ccgtagctgc
2281 cgtgccgtga gaggaggagg aggccgcctg ccgtgccggc tcacgtctgc cgctccgcca
2341 cgcatttctg gatgccgaca gcggagcaag tccaacggtg gagcggaact ctcgagaggg
2401 gtccagaggc agcgacagag atgccgtgcc gtctgcttcg cttggcccga cgcgacgctg
2461 ctggttcgct ggttggtgtc cgttagactc gtcgacggcg tttaacaggc tggcattatc
2521 tactcgaaac aagaaaaatg tttccttagt ttttttaatt tcttaaaggg tatttgttta
2581 atttttagtc actttatttt attctatttt atatctaaat tattaaataa aaaaactaaa
2641 atagagtttt agttttctta atttagaggc taaaatagaa taaaatagat gtactaaaaa
2701 aattagtcta taaaaaccat taaccctaaa ccctaaatgg atgtactaat aaaatggatg
2761 aagtattata taggtgaagc tatttgcaaa aaaaaaggag aacacatgca cactaaaaag
2821 ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc ctttagacca tgtctaactg
2881 ttcatttata tgattctcta aaacactgat attattgtag tactatagat tatattattc
2941 gtagagtaaa gtttaaatat atgtataaag atagataaac tgcacttcaa acaagtgtga
3001 caaaaaaaat atgtggtaat tttttataac ttagacatgc aatgctcatt atctctagag
3061 aggggcacga ccgggtcacg ctgcactgca ggaattcgat atcaagcttg gcactggccg
3121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
3181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
3241 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
3301 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
3361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
3421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
3481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
3541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
3601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
3661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
3721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
3781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
3841 ccacctagcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
3901 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
3961 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
4021 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
4081 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
4141 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
4201 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
4261 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
4321 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
4381 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
4441 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
4501 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
4561 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
4621 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
4681 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
4741 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
4801 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
4861 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
4921 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
4981 ttacgacata tgggcaaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
5041 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
5101 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
5161 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
5221 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
5281 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
5341 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
5401 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
5461 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
5521 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
5581 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
5641 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
5701 cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
5761 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
5821 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
5881 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
5941 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
6001 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
6061 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
6121 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
6181 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
6241 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
6301 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
6361 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
6421 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
6481 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
6541 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
6601 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
6661 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
6721 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
6781 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
6841 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
6901 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
6961 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
7021 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
7081 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
7141 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
7201 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
7261 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
7321 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
7381 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
7441 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
7501 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
7561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
7621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
7681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
7741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
7801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
7861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
7921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
7981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
8041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
8101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
8161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
8221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
8281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
8341 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
8401 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
8461 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
8521 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
8581 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
8641 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
8701 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
8761 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
8821 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
8881 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
8941 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
9001 ccttttcccg ttcaacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
9061 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
9121 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
9181 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
9241 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
9301 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
9361 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
9421 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
9481 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
9541 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
9601 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
9661 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
9721 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
9781 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
9841 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat
9901 cgacagatcc ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg
9961 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
10021 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
10081 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
10141 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg
10201 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
10261 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
10321 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
10381 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
10441 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
10501 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
10561 cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac
10621 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
10681 gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc
10741 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
10801 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
10861 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
10921 agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccggatc tgcgaaagct
10981 cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa
11041 tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt
11101 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
11161 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
11221 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
11281 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
11341 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
11401 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc
11461 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
11521 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
11581 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
11641 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
11701 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
11761 accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga
11821 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
11881 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
11941 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
12001 atgattac
//
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