pECMV-Usp4-m-FLAG Plasmid

PVT15625   2ug

pECMV-Usp4-m-FLAG Plasmid Information

Promoter CMV

Prokaryotic resistance Kan

Screening marker G418

Clone strain DH5a

Culture Conditions LB/37 Celsius degree

Forward primer CMV-F

Reverse primer pFLAG-CMV-R

pECMV-Usp4-m-FLAG Plasmid Description

pECMV-Usp4-m-FLAG Plasmid is a ORF expression plasmid

pECMV-Usp4-m-FLAG Plasmid Sequence

AACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCT
TCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACG
ACGTTGTAAAACGACGGCCAGTGCCAAGCTGATCTATACATTGAATCAATATTGGCAATTAGCCATATTAGTCATTGGTT
ATATAGCATAAATCAATATTGGCTATTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCA
TGTCCAATATGACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAG
CCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGA
CGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAA
ACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGC
CTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCA
TGGTGATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATT
GACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCA
AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGAC
TCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGCGGAAGGC
CGGGGCAGCCGTGAGCGACCGGATGTGGAGACCCAGAAGACGGAGCTCGGAGCCTTGATGGGGACCACGCTCCAACGTGG
GGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAAGTATGTGGGCTTTGACAGCTGGGACATGTACA
ATGTCGGGGAGCATAACCTGTTTCCTGGACCTATTGACAACTCCGGACTCTTCTCAGATCCTGAGAGTCAGACCTTGAAG
GAGCACTTAATCGATGAGCTGGACTATGTGCTGGTCCCAGCCGAAGCCTGGAATAAATTGCTGAATTGGTATGGCTGTGT
GGAGGGCCAGCAGCCTATTGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTGGAAGTGTATTTGC
TGGAGCTGAAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTAAGTTGCCATTTTAGCAAAGCAGACACCATTGCAACT
ATTGAGAAGGAGATGAGGAAGCTCTTCAACATCCCTGCAGAACGTGAAACACGGCTTTGGAACAAATACATGAGCAACAC
CTATGAGCAGTTGAGCAAGCTAGACAACACTATCCAGGATGCTGGGCTGTACCAGGGTCAGGTGCTAGTAATTGAGCCCC
AAAATGAAGATGGCACATGGCCCCGGCAGAGCCTGCAGTCAAAATCAAGCACTGCACCTAGCAGAAATTTCACTACCTCT
TCAAAACCATCCGCAAGTCCCTATTGCTCAGTGTCTGCCTCTCTCATTGCAAATGGTGATAGCACTAACAGCTCTGGGAT
GCACAGCTCCGGTGTCAGCAGGGGTGGATCTGGCTTCTCTGCTTCGTATAATTGCCAGGAGCCCCCATCACCTCATATAC
AGCCTGGCCTCTGTGGACTTGGAAACCTGGGGAACACTTGCTTCATGAACTCTGCTCTGCAGTGCCTGAGCAACACTGCC
CCACTGACTGAGTACTTTCTCAAAGATGAGTATGAGGCCGAGATCAACCGAGACAACCCTCTGGGGATGAAAGGGGAGAT
TGCAGAGGCCTATGCAGAGCTCATCAAGCAGATGTGGTCTGGAAGGGACACTCACGTGGCACCACGGATGTTCAAGACGC
AAGTGGGACGTTTTGCCCCTCAGTTTTCTGGCTACCAACAGCAAGACTCCCAGGAGCTGCTAGCCTTTATTCTAGACGGA
CTGCACGAGGACCTGAACCGCGTAAAGAAGAAGCCTTACCTGGAGCCCAAGGACGCCAATGGGCGACCAGATGCGGTGGT
AGCAAAGGAAGCCTGGGAAAACCACAGGCTGAGGAATGATTCTGTGATTGTGGATACTTTCCATGGCCTTTTCAAATCGA
CTTTGGTTTGCCCAGAATGTGCTAAAGTTTCTGTGACCTTTGACCCATTTTGCTATCTAACTCTCCCACTGCCTTTGAAG
AAGGATCGGATTATGGAGGTCTTCCTGGTTCCTGCTGACCCTCAGTGCAGACCTATCCAGTACCGTGTGACTGTGCCATT
GATGGGGGCCATTTCTGACCTGTGTGAAGCACTCTCCAAGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCACTGATG
TATATAATCACCGCTTCCACAAAATTTTCCAAATGGATGAAGGTTTAAGCCACATCACGCCTCGAGATGACATTTTTGTG
TATGAGGTCTGCAACACGTCCATGGATGGCTCAGAGTGTATCACTCTTCCAGTCTACTTCAGAGAGAAGAAGTCCAGGCC
GTCGAGTGCTTCCTCGGGGGCTGTGCTCTATGGACAGCCCCTTCTTGTCTCTGTCCCTAAGCATAAGCTAACCCTAGAGT
CTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTACATAAAACAGCCTTTGCCTGATGAGTTTCTCAGCTCACCCTTA
GAGCCTGGGGCCTGCAATGGCTCTAGGAGTAGCTATGAAGGAGATGAAGAGGAAGAAATGGATCATCAAGAGGAAGGAAA
AGAGCAGCTTTCCGAAGTGGAAGGCAGTGGTGAGGACGATCAGGGAGATGACCATAGCGAGAGCGCCCAAAAGGTGAAAG
GCCAGCCGAGGCACAAGAGGCTCTTTACCTTCAGCCTCGTGAACTCCTGTGGAACTGCTGATATCAATTCACTGGCAACT
GACGGGAAACTCCTCAAACTCAACTCTCGATCCACACTGGCCATTGACTGGGACAGTGAGACCCGAAGCCTTTACTTTGA
TGAGCAAG......
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Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.  Shipping temperature is 2-8℃.