pET28a-SIRT1(E230K) Plasmid 

Catalog No. PVTB00126-1b

Vector Backbone pET-28a(+)

Backbone manufacturer Novagen

Vector type E. coli Expression

Backbone size w/o insert 5369bp

Cloning site 5 BamH I

Site destroyed during cloning NO

Cloning site 3 Not I

Bacterial resistance Kanamycin

Growth strain DH5alpha

Growth temperature 37 Centigrade

High or low copy Low Copy

Selectable markers None

Background E. coli Expression vector of Homo sapiens sirtuin 1(one point mutation E230K) with N terminal 6xHis tag

Gene Information

Alternative Gene Name SIR2; SIR2L1; SIR2alpha

Insert Gene name SIRT1(E230K)

Insert size 2244bp

Accessions NM_012238

Tag His

Species Homo sapiens (human)

Gene ID 23411