pET9a-nirB-pelB-anti-PDL1-Nanobody-6×His plasmid

PVT103820

pET9a-nirB-pelB-anti-PDL1-Nanobody-6×His plasmid Information

Plasmid name: pET9a-nirB-pelB-anti-PDL1-Nanobody(opt-Ecoli)-6×His
Alias:
Gene lenght: 1077
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: E.coli
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: T7 terminator-R
Copy number: Low
Induction:

pET9a-nirB-pelB-anti-PDL1-Nanobody-6×His plasmid Description

pET9a-nirB-pelB-anti-PDL1-Nanobody-6×His is a plasmid suitable for protein expression in Escherichia coli.

pET9a-nirB-pelB-anti-PDL1-Nanobody-6×His plasmid Sequence

1 TTCTTAGAAA AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT CAGGATTATC 61 AATACCATAT TTTTGAAAAA GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT 121 CCATAGGATG GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA 181 ACCTATTAAT TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC 241 GACTGAATCC GGTGAGAATG GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG 301 CCAGCCATTA CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT TCATTCGTGA 361 TTGCGCCTGA GCGAGACGAA ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT 421 CGAATGCAAC CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG 481 ATATTCTTCT AATACCTGGA ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC 541 ATCATCAGGA GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA 601 GTTTAGTCTG ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC CATGTTTCAG 661 AAACAACTCT GGCGCATCGG GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC 721 GACATTATCG CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG 781 CGGCCTCGAG CAAGACGTTT CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT 841 TATGTAAGCA GACAGTTTTA TTGTTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC 901 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC 961 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG 1021 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA 1081 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC 1141 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT 1201 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA 1261 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC 1321 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC 1381 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT 1441 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT 1501 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC 1561 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 1621 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 1681 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC 1741 ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC 1801 CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC 1861 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT 1921 TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA 1981 CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG 2041 ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG 2101 CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC 2161 GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC 2221 ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA 2281 CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC 2341 GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG 2401 AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG 2461 AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT 2521 CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG 2581 GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG 2641 ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG 2701 GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT 2761 CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC 2821 GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT 2881 TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG 2941 TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT 3001 GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA 3061 TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT 3121 CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG 3181 TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA 3241 TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT 3301 GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC 3361 GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC 3421 CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG 3481 CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC 3541 CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT 3601 CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC 3661 CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTGAAT TTGATTTACA TCAATAAGCG 3721 GGGTTGCTGA ATCGTTAAGG Ttctagaaat aattttgttt aactttaaga aggagaatga 3781 aatacctgct gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg 3841 ccatgCAAGT TCAGCTGCAG GAATCCGGTG GTGGTCTGGT ACAACCTGGC GGCAGCCTGC 3901 GCCTGTCCTG CGCAGCAAGC GGCAAAATGT CTAGCCGTCG TTGCATGGCC TGGTTTCGTC 3961 AAGCGCCGGG CAAAGAGCGT GAACGTGTAG CTAAGCTGCT GACCACCTCC GGTAGCACTT 4021 ACCTGGCGGA CTCTGTTAAA GGCCGTTTCA CCATCAGCCA GAACAACGCC AAAAGCACTG 4081 TGTATCTGCA GATGAACTCC CTGAAGCCGG AAGACACTGC GATGTACTAC TGTGCAGCAG 4141 ATTCCTTCGA AGATCCAACC TGTACCCTGG TGACTTCCTC TGGTGCATTT CAGTACTGGG 4201 GTCAGGGCAC CCAAGTGACC GTTTCTGAGC CGAAATCTTG CGACAAAACC CACACTTGCC 4261 CTCCGTGCCC GGCGCCGGAA CTGCTGGGTG GCCCATCTGT GTTCCTGTTC CCACCGAAAC 4321 CGAAAGACAC CCTGATGATT TCTCGCACCC CGGAAGTGAC CTGCGTTGTG GTTGATGTCA 4381 GCCATGAGGA CCCGGAAGTG AAATTCAACT GGTACGTAGA TGGTGTTGAA GTTCACAACG 4441 CGAAAACCAA ACCGCGTGAA GAACAGTACA ACTCCACCTA TCGCGTAGTG TCCGTCCTGA 4501 CCGTTCTGCA CCAGGATTGG CTGAACGGTA AAGAATACAA GTGCAAAGTT TCTAACAAGG 4561 CTCTGCCGGC TCCGATTGAG AAAACTATCA GCAAAGCTAA AGGTCAACCG CGTGAACCAC 4621 AGGTGTATAC CCTGCCGCCG TCCCGTGACG AGCTGACCAA GAACCAGGTA AGCCTGACTT 4681 GTCTGGTTAA AGGCTTCTAC CCATCTGACA TCGCAGTTGA ATGGGAATCT AATGGCCAGC 4741 CGGAGAACAA CTACAAGACT ACCCCGCCAG TACTGGACAG CGACGGCTCC TTTTTCCTGT 4801 ACAGCAAACT GACCGTAGAC AAAAGCCGCT GGCAGCAGGG CAATGTCTTT TCTTGCAGCG 4861 TGATGCACGA AGCACTGCAT AACCACTACA CGCAAAAGAG CCTGTCTCTG TCCCCAGGTA 4921 AAcatcacca tcatcaccac taGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT 4981 TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT 5041 TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC 5101 GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG 5161 CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT 5221 AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG 5281 AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG 5341 AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT 5401 AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8???