pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S plasmid

PVT89655

pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S plasmid Information

Plasmid name: pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S
Alias: SC133183
Gene lenght: 3531bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Arabidopsis thaliana
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: LacI-R
Copy number: High
Induction: IPTG Induction

pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S plasmid Description

pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S is a plasmid suitable for protein expression in Escherichia coli.

pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S plasmid Sequence

1 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt 61 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca 121 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga 181 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac 241 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg 301 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc 361 tagaaataat tttgtttaac tttaagaagg agatataccA TGGCGACTCT AGACTCCGAC 421 GTTACCATGA TTCCTGCCGG AGAAGCCTCC AGCAGCGTAG CCGCGTCGTC TTCCAACAAG 481 AAAGCTAAGC GATTCGAAAT TAAGAAGTGG AGCGCCGTTG CTCTCTGGGC TTGGGATATC 541 GTTGTTGACA ACTGTGCGAT CTGCAGAAAC CACATCATGG ATCTTTGTAT CGAGTGTCAG 601 GCTAATCAGG CCAGTGCCAC AAGTGAAGAG TGCACTGTAG CTTGGGAGGA CGACCAAAAT 661 AACTGCAATA AGTATTTCTG TATTCTTGAT TGCTCAATGA AAGATGACCA CTTGGAGGGG 721 GTTTGCAATC ACGCCTTCCA CTTTCACTGC ATCAGCAGAT GGCTAAAGAC TCGTCAAGTT 781 TGTCCATTGG ATAACAGTGA GTGGGAGTTT CAGAAATATG GTCACagcca ggatccgaat 841 tcgatggcta gcatgactgg tggacagcaa atgggtCCAt aaCCCCGAAA GAAGGAGATA 901 TACATatgcc ttcaattaag ttgcagagtt ctgatggaga gatatttgaa gttgatgtgg 961 aaattgccaa acaatctgtg actattaaga ccatgttgga agatttggga atggatgatg 1021 aaggagatga tgacccagtt cctctaccaa atgtgaatgc agcaatatta aaaaaggtca 1081 ttcagtggtg cacccaccac aaggatgacc ctcctcctcc tgaagatgat gagaacaaag 1141 aaaagcgaac agatgatatc cctgtttggg accaagaatt cctgaaagtt gaccaaggaa 1201 cactttttga actcattctg gctgcaaact acttagacat caaaggtttg cttgatgtta 1261 catgcaagac tgttgccaat atgatcaagg ggaaaactcc tgaggagatt cgcaagacct 1321 tcaatatcaa aaatgacttt actgaagagg aggaagccca ggtaggtagc acacagtttt 1381 gtcttagctc ggcgcgcctg cagatggcta gcatgactgg tggacagcaa atgggtgtcg 1441 acaagcttgc ggccgcataa tgcttaagtc gaacagaaag taatcgtatt gtacacggcc 1501 gcataatcga aattaatacg actcactata ggggaattgt gagcggataa caattcccca 1561 tcttagtata ttagttaagt ataagAAGGA Gatatacata tggcagatct caattggata 1621 tcggccggcc acgcgatcgc tgacgtcggt accATGGAGC GCAAGACTAT TGACTTGGAG 1681 CAAGGATGGG ACTATATGCA GACTGGAATT ACTAAGCTGA AACGGATTCT GGAAGGATTG 1741 AATGAGCCAG CATTTGACTC TGAGCAATAT ATGATGCTTT ATACGACTAT CTACAACATG 1801 TGCACCCAGA AACCTCCTCA TGATTACTCA CAGCAGCTTT ATGACAAGTA TCGGGAAGCA 1861 TTTGAGGAAT ATATTAACTC AACAGTTTTG CCTGCGTTGA GGGAGAAGCA TGATGAGTTT 1921 ATGCTGCGGG AGCTATTTAA AAGATGGTCA AACCATAAAG TAATGGTCAG ATGGCTATCC 1981 CGCTTCTTCT ACTACCTTGA CCGTTACTTC ATTGCTCGGA GATCACTTCC ACCACTGAAT 2041 GAAGTTGGCC TGACATGCTT CCGTGACCTG GTTTATAATG AGCTACATTC TAAGGTCAAA 2101 CAAGCTGTAA TAGCTCTTGT TGATAAAGAA CGGGAGGGCG AGCAGATTGA TAGGGCCCTG 2161 CTGAAAAACG TATTAGATAT CTATGTAGAG ATTGGAATGG GGCAGATGGA GAGGTATGAA 2221 GAAGATTTTG AAAGCTTCAT GCTTCAAGAT ACTTCTTCGT ATTATTCTCG CAAGGCATCA 2281 AGCTGGATTC AGGAAGATTC TTGCCCTGAT TACATGTTGA AGTCTGAAGA ATGTCTAAAG 2341 AAGGAGAGGG AGAGAGTGGC TCACTACCTA CACTCAAGCA GTGAGCCAAA GCTGGTTGAG 2401 AAAGTACAAC ATGAATTGCT GGTTGTGTTT GCAAGTCAGC TTCTAGAAAA AGAACACTCA 2461 GGGTGCCGTG CATTGCTAAG AGATGACAAG GTGGATGATC TCTCCAGGAT GTACAGGCTT 2521 TACCATAAAA TTTTGCGAGG CTTGGAACCT GTTGCAAACA TCTTTAAGCA GCATGTCACA 2581 GCAGAGGGTA ACGCTCTTGT CCAACAGGCC GAAGACACGG CTACTAATCA GGTTGCAAAT 2641 ACTGCTAGCG TCCAGGAACA GGTTCTTATC AGAAAAGTGA TTGAACTTCA TGATAAATAC 2701 ATGGTATATG TCACCGAGTG TTTCCAGAAC CACACCCTCT TCCATAAGGC TTTGAAAGAG 2761 GCATTTGAGA TTTTTTGTAA CAAAACGGTT GCTGGAAGTT CAAGTGCAGA ACTACTTGCA 2821 ACATTTTGCG ACAATATTCT CAAAAAGGGG GGAAGTGAAA AGCTGAGTGA TGAAGCTATC 2881 GAAGATACGC TTGAGAAGGT TGTCAAATTG CTTGCATACA TAAGTGACAA GGATCTTTTC 2941 GCTGAGTTCT ACAGGAAGAA GCTGGCCCGT AGGCTCTTAT TTGATCGCAG TGCTAATGAT 3001 GATCATGAGA GAAGTATCCT GACAAAGCTC AAGCAACAAT GTGGTGGACA GTTTACTTCT 3061 AAGATGGAGG GCATGGTGAC GGATTTGACA CTGGCAAGAG AAAACCAAAA CAGTTTCGAG 3121 GATTATCTAG GCAGTAACCC TGCTGCAAAC CCAGGGATTG ACTTGACCGT CACTGTTCTT 3181 ACCACTGGTT TCTGGCCAAG TTACAAATCA TTTGACATAA ATCTACCCAG TGAAATGATC 3241 AAGTGTGTTG AAGTCTTCAA AGGGTTTTAT GAAACGAAAA CGAAACACAG GAAGCTTACG 3301 TGGATCTATT CACTGGGAAC TTGTCACATA AACGGGAAGT TTGATCAAAA GGCCATCGAG 3361 TTAATAGTGT CTACTTACCA GGCTGCTGTG CTTCTACTCT TTAACACAAC TGACAAGTTA 3421 AGTTACACTG AGATCTTGGC TCAACTGAAC CTAAGCCATG AAGATCTAGT TAGGTTGCTT 3481 CATTCCTTGT CATGTGCTAA GTACAAGATA CTCCTTAAGG AGCCAAACAC CAAGACTGTC 3541 TCCCAGAATG ATGCCTTTGA GTTCAACTCC AAATTCACCG ATAGAATGCG CAGAATCAAG 3601 ATCCCTCTTC CCCCAGTTGA TGAAAGGAAG AAAGTCGTTG AAGATGTCGA TAAAGACAGA 3661 AGATATGCAA TTGATGCTGC CATTGTCAGG ATCATGAAGA GCAGGAAAGT ATTGGGACAT 3721 CAACAACTTG TTTCTGAGTG TGTTGAGCAA CTTAGCCGAA TGTTCAAGCC TGATATCAAA 3781 GCGATCAAGA AGCGTATGGA GGATTTAATA ACCAGAGATT ATTTGGAGAG GGACAAGGAG 3841 AATCCTAACA TGTTTAGGTA CTTGGCTggt accctcgagt ctggtaaaga aaccgctgct 3901 gcgaaatttg aacgccagca catggactcg tctactagcg cagcttaatt aacctaggct 3961 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg 4021 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg 4081 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 4141 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 4201 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 4261 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 4321 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 4381 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 4441 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 4501 aaatattaac gtttacaatt tctggcggca cgatggcatg agattatcaa aaaggatctt 4561 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 4621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 4681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 4741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 4801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 4861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 4921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 4981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 5041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 5101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 5161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 5221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 5281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 5341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 5401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 5461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatcatgatt 5521 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5581 ataaacaaat aggtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 5641 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 5701 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 5761 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 5821 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 5881 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 5941 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 6001 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 6061 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 6121 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 6181 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 6241 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 6301 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 6361 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 6421 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt 6481 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 6541 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 6601 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 6661 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 6721 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg 6781 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa 6841 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg 6901 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt 6961 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg 7021 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga 7081 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt 7141 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca 7201 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat 7261 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga 7321 caggagcacg atcatgctag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 7381 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat 7441 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7501 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt 7561 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca 7621 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg 7681 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac 7741 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg 7801 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac 7861 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga 7921 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta 7981 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt 8041 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg 8101 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt 8161 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt 8221 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag 8281 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc 8341 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca 8401 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt 8461 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg 8521 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac 8581 cgcgaaaggt tttgcgccat tcgatggtgt c

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.