pEU-E01-MCS

LOCUS       Exported                3747 bp ds-DNA     circular SYN 11-SEP-2016

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pEU-E01-MCS

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 3747)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sunday, September 11, 2016 from SnapGene Viewer 3.1.4

FEATURES             Location/Qualifiers

     source          1..3747

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        1..19

                     /note="SP6 promoter"

                     /note="promoter for bacteriophage SP6 RNA polymerase"

     primer_bind     complement(834..850)

                     /note="M13 rev"

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     protein_bind    858..874

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="lac operator"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(882..912)

                     /note="lac promoter"

                     /note="promoter for the E. coli lac operon"

     protein_bind    927..948

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding site"

                     /note="CAP binding activates transcription in the presence 

                     of cAMP."

     rep_origin      complement(1236..1824)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(1995..2855)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIRYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(2856..2960)

                     /gene="bla"

                     /note="AmpR promoter"

ORIGIN

        1 atttaggtga cactatagaa ctcacctatc tccccaacac ctaataacat tcaatcactc

       61 tttccactaa ccacctatct acatcaccaa gatatcacta gttctcgagc tcggtacctg

      121 tccgcggtcg cgacgtacgc gggcggccgc cataaattgg atccatatat agggcccggg

      181 ttataattac ctcaggtcga cgtcccatgg ttttgtatag aatttacggc tagcgccgga

      241 tgcgacgccg gtcgcgtctt atccggcctt cctatatcag gcggtgttta agacgccgcc

      301 gcttcgccca aatccttatg ccggttcgac gactggacaa aatactgttt atcttcccag

      361 cgcaggcagg ttaatgtacc accccagcag cagccggtat ccagcgcgta tataccttcc

      421 ggcgtacctt tgccctccag cgatgcccag tgaccaaagg cgatgctgta ttcttcagcg

      481 acagggccag gaatcgcaaa ccacggtttc agtggggcag gggcctcttc cggcgattct

      541 tactagctag tatgcatagg tgctgaaata taaagtttgt gtttctaaaa cacacgtggt

      601 acgtacgata acgtacagtg tttttccctc cacttaaatc gaagggtagt gtcttggagc

      661 gcgcggagta aacatatatg gttcatatat gtccgtaggc acgtaaaaaa agcgagggat

      721 tcgaattccc ccggaacccc cggttggggc ccacgcctcg atcgagcaaa aaaaaaaaaa

      781 aagaaaaaaa aaaaaaaaaa aagctttccc gcggccagct tggcgtaatc atggtcatag

      841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc

      901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc

      961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa

     1021 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcact cactgactcg

     1081 ctgcgctcgg tcgctcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg

     1141 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag

     1201 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac

     1261 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga

     1321 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt

     1381 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc

     1441 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc

     1501 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta

     1561 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat

     1621 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca

     1681 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct

     1741 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt

     1801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct

     1861 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc

     1921 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa

     1981 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta

     2041 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc

     2101 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat

     2161 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta

     2221 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt

     2281 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt

     2341 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg

     2401 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc

     2461 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc

     2521 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagcgtatg

     2581 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga

     2641 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta

     2701 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct

     2761 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag

     2821 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga

     2881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat

     2941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc

     3001 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg

     3061 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct

     3121 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc

     3181 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat

     3241 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc

     3301 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc

     3361 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg

     3421 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc

     3481 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct

     3541 gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg

     3601 tccaaccaaa ccgactctgg cggcagttta cgagagagat gatagggtct gcttcagtaa

     3661 gccagatgct acacaattag gcttgtacat actgtcgtta gaacgcggct acaattaata

     3721 cataacctta tgtatcatac acatacg

//

 

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.  Shipping temperature is 2-8℃.