pEX18TC
PVT10636 Packing 2ug
pEX18TC Information
Carrier name: pEX18Tc, pEX-18Tc
Plasmid type: Suicide Plasmid
Cloning method: restriction endonuclease, polyclonal site
Promoter: Lac
Carrier size: 6349 BP
5'sequencing primers and sequences: M13F (-47) CGCCAGGGTTTTCCCAGTCACGAC
3'sequencing primers and sequences: M13R (-48) AGCGGATAACAATTTCACACAGGA
Carrier resistance: tetracycline
Virus / non virus: non viral
Function E.coli Editing plasmids
pEX18TC Reference
1. Biswas I., Mettlach J. (2019) Targeted Gene Replacement in Acinetobacter baumannii. In: Biswas I., Rather P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY
DOI https://doi.org/10.1007/978-1-4939-9118-1_10
Publisher Name Humana Press, New York, NY
Cloning vector pEX18Tc, complete sequence
LOCUS AF047519 6349 bp DNA circular SYN 25-NOV-2003
DEFINITION Cloning vector pEX18Tc, complete sequence.
ACCESSION AF047519
VERSION AF047519.1
KEYWORDS .
SOURCE Cloning vector pEX18Tc
ORGANISM Cloning vector pEX18Tc
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6349)
AUTHORS Hoang,T.T., Karkhoff-Schweizer,R.R., Kutchma,A.J. and
Schweizer,H.P.
TITLE A broad-host-range Flp-FRT recombination system for site-specific
excision of chromosomally-located DNA sequences: application for
isolation of unmarked Pseudomonas aeruginosa mutants
JOURNAL Gene 212 (1), 77-86 (1998)
PUBMED 9661666
REFERENCE 2 (bases 1 to 6349)
AUTHORS Hoang,T.T., Karkhoff-Schweizer,R.R., Kutchma,A.J. and
Schweizer,H.P.
TITLE Direct Submission
JOURNAL Submitted (10-FEB-1998) Microbiology, Colorado State University,
Fort Collins, CO 80523, USA
FEATURES Location/Qualifiers
source 1..6349
/organism="Cloning vector pEX18Tc"
/mol_type="genomic DNA"
/db_xref="taxon:73160"
gene complement(209..1630)
/gene="sacB"
CDS complement(209..1630)
/gene="sacB"
/codon_start=1
/transl_table=11
/product="levansucrase precursor"
/protein_id="AAC27026.1"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
HITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG
YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
IKGKKTSVVKDSILEQGQLTVNK"
oriT 2104..2928
regulatory complement(2980..3006)
/regulatory_class="terminator"
/note="T2"
regulatory complement(3139..3182)
/regulatory_class="terminator"
/note="T1"
rRNA complement(3186..3305)
/product="Eschericia coli 5S ribosomal RNA"
gene complement(3341..3625)
/gene="lacZalpha"
CDS complement(3341..3625)
/gene="lacZalpha"
/codon_start=1
/transl_table=11
/product="lac alpha peptide"
/protein_id="AAC27027.1"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRAWLFWRMREDFQPDTD"
gene complement(4885..6075)
/gene="tet"
CDS complement(4885..6075)
/gene="tet"
/codon_start=1
/transl_table=11
/product="tetracycline efflux protein"
/protein_id="AAC27028.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataatcg gcattttctt ttgcgttttt atttgttaac tgttaattgt ccttgttcaa
241 ggatgctgtc tttgacaaca gatgttttct tgcctttgat gttcagcagg aagctaggcg
301 caaacgttga ttgtttgtct gcgtagaatc ctctgtttgt catatagctt gtaatcacga
361 cattgtttcc tttcgcttga ggtacagcga agtgtgagta agtaaaggtt acatcgttag
421 gatcaagatc catttttaac acaaggccag ttttgttcag cggcttgtat gggccagtta
481 aagaattaga aacataacca agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca
541 tttttgatcc gcgggagtca gtgaacagat accatttgcc gttcatttta aagacgttcg
601 cgcgttcaat ttcatctgtt actgtgttag atgcaatcag cggtttcatc acttttttca
661 gtgtgtaatc atcgtttagc tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc
721 gttttttatc gctttgcaga agtttttgac tttcttgacg gaagaatgat gtgcttttgc
781 catagtatgc tttgttaaat aaagattctt cgccttggta gccatcttca gttccagtgt
841 ttgcttcaaa tactaagtat ttgtggcctt tatcttctac gtagtgagga tctctcagcg
901 tatggttgtc gcctgagctg tagttgcctt catcgatgaa ctgctgtaca ttttgatacg
961 tttttccgtc accgtcaaag attgatttat aatcctctac accgttgatg ttcaaagagc
1021 tgtctgatgc tgatacgtta acttgtgcag ttgtcagtgt ttgtttgccg taatgtttac
1081 cggagaaatc agtgtagaat aaacggattt ttccgtcaga tgtaaatgtg gctgaacctg
1141 accattcttg tgtttggtct tttaggatag aatcatttgc atcgaatttg tcgctgtctt
1201 taaagacgcg gccagcgttt ttccagctgt caatagaagt ttcgccgact ttttgataga
1261 acatgtaaat cgatgtgtca tccgcatttt taggatctcc ggctaatgca aagacgatgt
1321 ggtagccgtg atagtttgcg acagtgccgt cagcgttttg taatggccag ctgtcccaaa
1381 cgtccaggcc ttttgcagaa gagatatttt taattgtgga cgaatcgaac tcaggaactt
1441 gatatttttc atttttttgc tgttcaggga tttgcagcat atcatggcgt gtaatatggg
1501 aaatgccgta tgtttcctta tatggctttt ggttcgtttc tttcgcaaac gcttgagttg
1561 cgcctcctgc cagcagtgcg gtagtaaagg ttaatactgt tgcttgtttt gcaaactttt
1621 tgatgttcat cgttcatgtc tcctttttta tgtactgtgt tagcggtctg cttcttccag
1681 ccctcctgtt tgaagatggc aagttagtta cgcacaataa aaaaagacct aaaatatgta
1741 aggggtgacg ccaaagtata cactttgccc tttacacatt ttaggtcttg cctgctttat
1801 cagtaacaaa cccgcgcgat ttacttttcg acctcattct attagactct cgtttggatt
1861 gcaactggtc tattttcctc ttttgtttga tagaaaatca taaaaggatt tgcagactac
1921 gggcctaaag aactaaaaaa tctatctgtt tcttttcatt ctctgtattt tttatagttt
1981 ctgttgcatg ggcataaagt tgccttttta atcacaattc agaaaatatc ataatatctc
2041 atttcactaa ataatagtga acggcaggta tatgtgatgg gttaaaaagg atcgatcctc
2101 tagctagagt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
2161 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
2221 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
2281 ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc
2341 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
2401 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
2461 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
2521 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
2581 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
2641 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc
2701 ttccctgctg ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg
2761 aactgagggg acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg
2821 gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg
2881 gtgagggcgg atgtcgatat gcgtaaggag aaaataccgc atcaggcgca tatttgaatg
2941 tatttagaaa aataaacaaa aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
3001 gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
3061 ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
3121 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
3181 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
3241 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc
3301 aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa
3361 aatcttctct catccgccaa aacagccaag ctcgccattc gccattcagg ctgcgcaact
3421 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
3481 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
3541 cgacggccag tgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
3601 agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
3661 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
3721 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
3781 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
3841 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
3901 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
3961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
4021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
4081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
4141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
4201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
4261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
4321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
4381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
4441 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
4501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
4561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
4621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
4681 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
4741 aaatcaatct aaagtatata tgagtaaact tggtctgaca atcgatgcaa gggttggttt
4801 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
4861 tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg
4921 caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
4981 tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag
5041 gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
5101 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
5161 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
5221 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
5281 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
5341 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
5401 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
5461 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcggc gctctccctt
5521 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
5581 cgcaaggaat ggtgcatgta aggagatggc gcccaacagt cccccggcca cggggcctgc
5641 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
5701 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
5761 cacgatgcgt ccggcgtaga gaatccacag gacgggtgtg gtcgccatga tcgcgtagtc
5821 gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag
5881 tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc
5941 atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg
6001 cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc
6061 attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc
6121 gcattaaagc taatcggttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
6181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
6241 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
6301 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
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