pEYFP-Actin encodes a fusion of the enhanced yellow fluorescent protein (EYFP) and human cytoplasmic β-actin. EYFP is an enhanced yellow-green variant of the Aequorea victoria green fluorescent protein (GFP). The EYFP gene contains the four amino acid substitutions previously published as GFP-10C: Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. It also contains the Phe-64 to Leu mutation of GFPmut1. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 513 nm, the Em of EYFP is 36,500 cm–1M–1 and the fluorescence quantum yield is 0.63, resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that from EGFP
LOCUS Exported 5820 bp ds-DNA circular SYN 19-10-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled 4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5820)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-10-19 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5820
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/codon_start=1
/product="enhanced YFP"
/note="EYFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 2608..2729
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2736..3191)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3218..3322
/gene="bla"
/note="AmpR promoter"
promoter 3324..3681
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3532..3667
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3716..4510
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4742..4789
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 5118..5706
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atctcgagct atggatgatg atatcgccgc gctcgtcgtc
1381 gacaacggct ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc
1441 ttcccctcca tcgtggggcg ccccaggcac cagggcgtga tggtgggcat gggtcagaag
1501 gattcctatg tgggcgacga ggcccagagc aagagaggca tcctcaccct gaagtacccc
1561 atcgagcacg gcatcgtcac caactgggac gacatggaga aaatctggca ccacaccttc
1621 tacaatgagc tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga ggcccccctg
1681 aaccccaagg ccaaccgcga gaagatgacc cagatcatgt ttgagacctt caacacccca
1741 gccatgtacg ttgctatcca ggctgtgcta tccctgtacg cctctggccg taccactggc
1801 atcgtgatgg actccggtga cggggtcacc cacactgtgc ccatctacga ggggtatgcc
1861 ctcccccatg ccatcctgcg tctggacctg gctggccggg acctgactga ctacctcatg
1921 aagatcctca ccgagcgcgg ctacagcttc accaccacgg ccgagcggga aatcgtgcgt
1981 gacattaagg agaagctgtg ctacgtcgcc ctggacttcg agcaagagat ggccacggct
2041 gcttccagct cctccctgga gaagagctac gagctgcctg acggccaggt catcaccatt
2101 ggcaatgagc ggttccgctg ccctgaggca ctcttccagc cttccttcct gggcatggag
2161 tcctgtggca tccacgaaac taccttcaac tccatcatga agtgtgacgt ggacatccgc
2221 aaagacctgt acgccaacac agtgctgtct ggcggcacca ccatgtaccc tggcattgcc
2281 gacaggatgc agaaggagat cactgccctg gcacccagca caatgaagat caagatcatt
2341 gctcctcctg agcgcaagta ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc
2401 accttccagc agatgtggat cagcaagcag gagtatgacg agtccggccc ctccatcgtc
2461 caccgcaaat gcttctaggg atccaccgga tctagataac tgatcataat cagccatacc
2521 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
2581 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
2641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
2701 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
2761 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
2821 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
2881 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
2941 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
3001 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
3061 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
3121 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
3181 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
3241 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
3301 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
3361 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
3421 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3481 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
3541 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
3601 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
3661 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
3721 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
3781 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
3841 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
3901 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
3961 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
4021 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
4081 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
4141 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
4201 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
4261 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
4321 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
4381 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
4441 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
4501 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
4561 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
4621 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
4681 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
4741 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
4801 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
4861 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
4921 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
4981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
5041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
5101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
5161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
5221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
5281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
5341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
5401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
5461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
5521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
5581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
5641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
5701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
5761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.
Search name
pEYFP-Actin,Plasmid pEYFP-Actin,pEYFP-Actin vector
English