pFC333 Plasmid

PVT46695  2ug

pFC333 Plasmid Information

Host: filamentous fungi

Use(s): Gene Editing

Fragment type: CRISPR, Cas9

Prokaryotic resistance: Amp

Screen Marker: Zeo

Replicon: pUC

Competent cells: Stbl3

Temperature: 37℃

pFC333 Plasmid Sequence

TTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCG
TGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGCGCTGCGATGATACCGCGAGAACCACGCTCACCGGCT
CCGGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCA
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATCGCTACAG
GCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCC
CCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACT
CATGGTTATGGCAGCGCTACATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAA
CCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACAT
AGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATC
CAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAA
CAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATATTCTTCCTTTTTCAATAT
TATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGT
CAGTGTTACAACCAATTAACCAATTCTGAACATTATCGCGAGCCCATTTATACCTGAATATGGCTCATAACACCCCTTGT
TTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAG
TGTGGGGACTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTT
CGCCCGGGCTAATTATGGGGTGTCGCCCTTATTCGACTCTATAGTGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCT
GAAGTGGGGATTTAAATGCGGCCGCGCTGAGGGTTTAATCGACGAAGCAGCTGACGGCCAGTGCCAAGCTTAACGCGTAC
CGGGCCCAGTATATGTTCCGCAGATGACTGGAGCTCTGCCATACGTGCCCTCTCAAGCACCATTTGTTCCATCTACAGAG
ACTAGTCACCAACTAGTCTATCAAGACTCACAGGGTACATTGCTGAGACCAACTGACCAGAGGCAGGGTAGCGGATTGAC
GGCTCCATCTCCTTCACTTACAAGGTCTATTGAAAGCCCTTTAGCATCACCAAGCGGAGAATAGATTGTTAAGCTTATTT
TTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTAC
ATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGAC
GTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGT
TTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACAAAAAAA
AAAAACAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCAC
AGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTA
GTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGT
GATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTG
CCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGAC
TAGTCAAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCAT
GTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCA
GGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGG
TTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTG
CTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTGGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGG
TATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACCCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTAT
AAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCYTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCA
AATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCAT
TGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGA
CTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTG
CTGCTATATATTAGCTAATACACTAGTTAGACTCACAGAAACTTACGCAGCTCGCTTGCGCTTCTTGGTAGGAGTCGGGG
TTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTACTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGAT
AGGACTTGCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTC
TTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAG
TATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCC
ATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAA
GCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATA
CTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAG
CGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTT
CGACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGGGAGGAAAGAAAAAGTCGG
TCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCA
GGGAATTTAGGGAGTTATCTGCGACCACCGAGGAACGGCGGAATGCCAAAGAATCCCGATGGAGCTCTAGCTGGCGGTTG
ACAACCCCACCTTTTGGCGTTTCTGCGGCGTTGCAGGCGGGACTGGATACTTCGTAGAACCAGAAAGGCAAGGCAGAACG
CGCTCAGCAAGAGTGTTGGAAGTGATAGCATGATGTGCCTTGTTAACTAGGTCAAAATCTGCAGTATGCTTGATGTTATC
CGAAGTGTGAGAGAGGAAGGTCCAAACATACACGATTGGGAGAGGGCCTAGGTATAAGAGTTTTTGAGTAGAACGCATGT
GAGCCCAGCCATCTCGAGGAGATTAAACACGGGCCGGCATTTGATGGCTATGTTAGTACCCCAATGGAAACGGTGAGAGT
CCAGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAA
AAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCC
TATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTG
CTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCT
CGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGT
CACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGC
CTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCCATATGAAGTCTACTGCCGAGTGAAGGGAT
GCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACA
GAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATG
AAGGAACTGATGGAGGCCAAAGGCAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGTAGCATGGTAT
GAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGCGCAAGCGAGCTGCGTAAGTTTCTGTGAG
TCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTT
TCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGAC
AATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTAC
GCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAARGGCAAGTTACAT
TCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGGGTAGCAAGGC
AGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCCAAGC
TAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATC
GATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTT
CTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTA
GTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTC
TCCTTTGTGGTTCTGGACTAGTCTTTGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGG
CTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAG
TCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTAT
TCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGAGACTTTGGCCTCA
ATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACT
AGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTCTAGGTTCTGT
TAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTT
AATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAACTTTTACCCTAAAGTAGCTTGCTTGTGTG
TTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGA
AAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCTTAACAATCTATTCTCCGCTTGGTGATGCTAAAGGGCTT
TCAATAGACCTTGTAAGTGAAGGAGATGGAGCCGTCAATCCGCTACCCTGCCTCTGGTCAGTTGGTCTCAGCAATGTACC
CTGTGAGTCTTGATAGACTAGTTGGTGACTAGTCTCTGTAGATGGAACAAATGGTGCTTGAGAGGGCACGTATGGCAGAG
CTCCAGTCATCTGCGGAACATATACTGGGCCCGGGAAGATCTCATGGTCATAGCTGTTTCCGCTGAGGGTTTAATTAAGA
CCTCAGCCGAGACAGCAGAATCACCGCCCAAGTTAAGCCTTTGTGCTGATCATGCTCTCGAACGGGCCAAGTTCGGGAAA
AGCAAAGGAGCGTTTAGTGAGGGGCAATTTGACTCACCTCCCAGGCAACAGATGAGGGGGGCAAAAAGAAAGAAATTTTC
GTGAGTCAATATGGATTCCGAGCATCATTTTCTTGCGGTCTATCTTGCTACGTATGTTGATCTTGACGCTGTGGATCAAG
CAACGCCACTCGCTCGCTCCATCGCAGGCTGGTCGCAGACAAATTAAAAGGCGGCAAACTCGTACAGCCGCGGGGTTGTC
CGCTGCAAAGTACAGAGTGATAAAAGCCGCCATGCGACCATCAACGCGTTGATGCCCAGCTTTTTCGATCCGAGAATCCA
CCGTAGAGGCGATAGCAAGTAAAGAAAAGCTAAACAAAAAAAAATTTCTGCCCCTAAGCCATGAAAACGAGATGGGGTGG
AGCAGAACCAAGGAAAGAGTCGCGCTGGGCTGCCGTTCCGGAAGGTGTTGTAAAGGCTCGACGCCCAAGGTGGGAGTCTA
GGAGAAGAATTTGCATCGGGAGTGGGGCGGGTTACCCCTCCATATCCAATGACAGATATCTACCAGCCAAGGGTTTGAGC
CCGCCCGCTTAGTCGTCGTCCTCGCTTGCCCCTCCATAAAAGGATTTCCCCTCCCCCTCCCACAAAATTTTCTTTCCCTT
CCTCTCCTTGTCCGCTTCAGTACGTATATCTTCCCTTCCCTCGCTTCTCTCCTCCATCCTTCTTTCATCCATCTCCTGCT
AACTTCTCTGCTCAGCACCTCTACGCATTACTAGCCGTAGTATCTGAGCACTTCTCCCTTTTATATTCCACAAAACATAA
CACAACCTTCACCATGGACAAGAAGTATAGCATCGGGCTGGACATTGGAACGAACTCGGTTGGTTGGGCTGTGATTACGG
ACGAATACAAGGTGCCATCCAAGAAGTTTAAGGTCCTGGGAAACACCGACCGTCACTCAATCAAGAAGAATCTCATTGGA
GCCCTGCTCTTCGATAGTGGGGAGACCGCCGAAGCTACTCGACTGAAGCGAACGGCTCGCCGGCGTTATACACGACGCAA
GAATCGCATCTGCTACCTCCAGGAGATTTTCAGCAACGAAATGGCTAAGGTTGATGACTCATTCTTTCATCGACTCGAAG
AAAGTTTCTTGGTCGAGGAGGATAAGAAGCACGAGCGCCATCCGATCTTTGGTAACATTGTGGATGAGGTTGCCTATCAC
GAAAAGTACCCAACTATCTATCATCTTCGTAAGAAGCTGGTCGATAGCACGGACAAGGCTGATTTGCGACTTATCTACCT
GGCACTCGCGCACATGATTAAGTTCCGCGGCCATTTTCTTATCGAGGGTGACCTGAACCCCGATAATTCTGACGTTGATA
AGCTCTTCATCCAGTTGGTCCAAACCTACAATCAGCTGTTTGAGGAAAACCCTATTAATGCATCTGGCGTGGACGCCAAG
GCTATCCTTTCGGCGCGCCTGTCTAAGTCGCGGCGTTTGGAGAACCTTATCGCACAACTCCCCGGCGAAAAGAAGAACGG
CCTCTTCGGTAATTTGATTGCGTTGTCACTTGGTCTGACTCCTAACTTCAAGAGTAATTTTGACCTGGCAGAGGATGCGA
AGCTCCAGTTGTCTAAGGATACGTATGATGACGATCTCGACAACTTGCTTGCCCAAATCGGTGACCAGTACGCTGATCTT
TTCCTGGCCGCTAAGAATCTCTCAGATGCAATCCTGCTCAGTGACATTTTGCGGGTCAACACCGAGATTACTAAGGCCCC
CCTGTCAGCTAGTATGATCAAGCGGTATGATGAGCACCATCAGGACCTCACCTTGCTTAAGGCCCTCGTGCGTCAGCAAT
TGCCTGAGAAGTATAAGGAAATCTTCTTTGACCAATCCAAGAACGGATACGCAGGGTATATTGATGGCGGTGCGAGCCAG
GAGGAATTCTACAAGTTTATCAAGCCGATTTTGGAGAAGATGGACGGCACTGAGGAACTGCTCGTCAAGCTGAATCGCGA
AGATTTGCTTCGTAAGCAACGAACGTTCGACAACGGCTCCATCCCGCACCAGATTCATCTGGGCGAGCTCCACGCCATCC
TTCGACGCCAGGAAGATTTCTACCCATTTCTGAAGGACAACCGTGAGAAGATCGAAAAGATTCTTACATTCCGAATCCCC
TACTATGTGGGACCTTTGGCCCGTGGGAATTCCCGATTTGCTTGGATGACCCGAAAGAGCGAGGAAACCATCACTCCGTG
GAACTTCGAGGAAGTCGTGGACAAGGGTGCATCCGCGCAGAGCTTCATTGAGCGGATGACCAATTTTGATAAGAACCTTC
CGAATGAAAAGGTCCTGCCAAAGCATTCGCTGCTCTACGAGTATTTCACCGTGTATAACGAACTGACTAAGGTCAAGTAC
GTGACGGAGGGAATGCGGAAGCCAGCCTTCCTCTCAGGGGAACAAAAGAAGGCTATCGTCGATTTGCTTTTTAAGACCAA
TCGTAAAGTGACTGTTAAGCAGCTGAAGGAGGATTATTTCAAGAAGATTGAATGTTTCGACTCCGTCGAGATCAGCGGCG
TGGAAGATCGCTTTAACGCTTCCCTCGGTACCTACCACGACCTGCTCAAGATCATTAAGGACAAGGATTTCCTCGATAAC
GAGGAAAATGAGGACATCTTGGAAGATATTGTCCTCACGTTGACACTTTTTGAGGACCGCGAAATGATCGAGGAACGGCT
CAAGACATATGCCCATTTGTTCGACGATAAGGTGATGAAGCAGCTGAAGCGGCGTCGATACACCGGATGGGGTCGCCTTA
GCCGGAAGCTGATCAACGGCATTCGAGATAAGCAATCTGGTAAGACTATCTTGGATTTCCTTAAGTCGGACGGCTTCGCC
AACCGCAATTTTATGCAGCTTATTCACGACGATTCCCTGACGTTCAAGGAGGACATCCAGAAGGCACAAGTCTCAGGACA
AGGGGATTCCCTGCACGAGCATATCGCCAACCTGGCTGGATCCCCGGCGATCAAGAAGGGGATTCTTCAGACCGTCAAGG
TTGTCGACGAGCTGGTCAAGGTGATGGGCCGTCATAAGCCAGAAAACATCGTGATTGAGATGGCCCGAGAAAATCAGACC
ACTCAAAAGGGTCAGAAGAACAGCCGCGAGCGGATGAAGCGGATCGAGGAAGGCATTAAGGAACTTGGTTCTCAGATCCT
GAAGGAGCACCCTGTTGAAAACACACAGCTCCAAAATGAGAAGCTGTATCTCTACTATTTGCAAAATGGACGCGACATGT
ACGTCGATCAGGAGCTCGACATTAACCGGTTGTCGGACTACGATGTTGACCATATCGTCCCGCAATCCTTCCTTAAGGAC
GATAGCATTGATAACAAGGTGCTGACTCGCTCAGATAAGAACCGGGGCAAGTCCGACAATGTTCCAAGCGAGGAAGTGGT
TAAGAAGATGAAGAACTACTGGCGCCAATTGCTTAATGCCAAGCTCATCACACAGCGCAAGTTTGACAACTTGACCAAGG
CCGAGCGGGGAGGGCTGAGTGAACTCGATAAGGCTGGCTTCATCAAGCGTCAACTCGTGGAGACGCGACAGATCACAAAG
CACGTTGCTCAGATTCTGGACTCCCGGATGAACACAAAGTACGACGAGAATGATAAGCTCATCCGTGAAGTTAAGGTCAT
TACCCTCAAGTCTAAGTTGGTGTCGGATTTCCGCAAGGACTTCCAATTTTATAAGGTTCGGGAGATCAACAATTATCACC
ATGCACATGATGCGTACCTCAACGCAGTCGTGGGAACTGCGCTCATCAAGAAGTATCCCAAGTTGGAGTCCGAATTCGTC
TACGGGGATTATAAGGTTTACGACGTCCGCAAGATGATCGCCAAGAGTGAGCAGGAAATTGGCAAGGCCACGGCTAAGTA
TTTCTTTTACTCCAACATCATGAATTTCTTTAAGACGGAGATCACACTCGCCAATGGAGAAATCCGTAAGCGACCTTTGA
TTGAGACCAACGGCGAGACTGGTGAAATCGTTTGGGATAAGGGGCGCGACTTCGCTACCGTGCGGAAGGTTCTGAGCATG
CCGCAAGTCAATATCGTCAAGAAAACCGAGGTGCAGACAGGCGGTTTCTCTAAGGAATCGATTCTTCCAAAGCGTAACTC
TGACAAGCTGATCGCTCGAAAGAAGGATTGGGACCCCAAGAAGTATGGAGGGTTCGATTCTCCTACAGTGGCATACTCGG
TTCTCGTTGTCGCGAAGGTTGAGAAGGGAAAGTCTAAGAAGCTGAAGTCGGTCAAGGAACTGCTCGGGATCACCATTATG
GAGCGCTCCAGCTTCGAAAAGAATCCCATCGACTTTCTCGAGGCCAAGGGCTATAAGGAAGTCAAGAAGGATCTTATCAT
TAAGCTGCCTAAGTACTCTTTGTTCGAGCTTGAAAACGGTCGAAAGCGAATGCTCGCATCGGCAGGAGAGTTGCAGAAGG
GGAATGAATTGGCACTTCCCTCAAAGTACGTGAACTTCCTGTATCTCGCGTCCCACTACGAGAAGCTGAAGGGTAGCCCT
GAGGACAACGAACAGAAGCAACTTTTTGTTGAGCAACACAAGCATTATCTGGATGAGATCATTGAACAGATTTCAGAGTT
CAGTAAGCGCGTCATCCTCGCCGATGCTAATCTCGACAAGGTGTTGTCGGCCTACAACAAGCACCGTGACAAGCCGATCC
GAGAGCAGGCTGAAAATATCATTCATCTGTTCACCCTCACTAACTTGGGAGCACCAGCAGCGTTCAAGTATTTTGATACG
ACAATCGACCGTAAGCGATACACGTCCACAAAGGAGGTGCTTGATGCGACCCTGATTCATCAATCCATCACTGGGCTCTA
TGAAACCCGTATCGACCTTAGTCAACTGGGGGGCGACCCTCCCAAGAAGAAGCGCAAGGTCTGAGCGGACATTCGATTTA
TGCCGTTATGACTTCCTTAAAAAAGCCTTTACGAATGAAAGAAATGGAATTAGACTTGTTATGTAGTTGATTCTACAATG
GATTATGATTCCTGAACTTCAAATCCGCTGTTCATTATTAATCTCAGCTCTTCCCGTAAAGCCAATGTTGAAACTATTCG
TAAATGTACCTCGTTTTGCGTGTACCTTGCTTATCACGTGATATTACATGACCTGGACAGAGTTCTGCGCGAAAGTCATA
ACGTAAATCCCGGGCGGTAGGTGCGTCCCGGGCGGAAGGTAGTTTTCTCGTCCACCCCAACGCGTTTATCAACCTCAACT
TTCAACAACCATCATGCCACCAAAAGCGCGTAAAACAAAGCGAGATTTGATTGAGCAAGAGGGCAGGATCCAATGCGCGA
TTCAAGACATTAAAAATGGAAAATTTCAAAAAATTGCGCCCGCAGCGCGTGCATACAAAATTCATCCCAATACAAGTGTA
ATGCTAGTGGAGGTCAACACATCAATGCCTATTTTGGTTTAGTCGTCCAGGCGGTGAGCACAAAATTTGTGTCGTTTGAC
AAGATGGTTCATTTAGGCAACTGGTCAGATCAGCCCCACTTGTAGCAGTAGCGGCGGCGCTCGAAGTGTGACTCTTATTA
GCAGACAGGAACGAGGACATTATTATCATCTGCTGCTTGGTGCACGATAACTTGGTGCGTTTGTCAAGCAAGGTAAGTGG
ACGACCCGGTCATACCTTCTTAAGTTCGCCCTTCCTCCCTTTATTTCAGATTCAATCTGACTTACCTATTCTACCCAAGC
ATCCAAATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGA
CCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCG
CGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGG
TCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGA
GTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGATTCCGGATCCACTTAACGT
TACTGAAATCATCAAACAGCTTGACGAATCTGGATATAAGATCGTTGGTGTCGATGTCAGCTCCGGAGTTGAGACAAATG
GTGTTCAGGATCTCGATAAGATACGTTCATTTGTCCAAGCAGCAAAGAGTGCCTTCTAGTGATTTAATAGCTCCATGTCA
ACAAGAATAAAACGCGTTTCGGGTTTACCTCTTCCAGATACAGCTCATCTGCAATGCATTAATGCATTGGACCTCGCAAC
CCTAGTACGCCCTTCAGGCTCCGGCGAAGCAGAAGAATAGCTTAGCAGAGTCTATTTTCATTTTCGGGAGACGAGATCAA
GCAGATCAACGGTCGTCAAGAGACCTACGAGACTGAGGAATCCGCTCTTGGCTCCACGCGACTATATATTTGTCTCTAAT
TGTACTTTGACATGCTCCTCTTCTTTACTCTGATAGCTTGACTATGAAAATTCCGTCACCAGCCCCTGGGTTCGCAAAGA
TAATTGCACTGTTTCTTCCTTGAACTCTCAAGCCTACAGGACACACATTCATCGTAGGTATAAACCTCGAAAATCATTCC
TACTAAGATGGGTATACAATAGTAACCATGCATGGTTGCCTAGTGAATGCTCCGTAACACCCAATACGCCGGCCGAAACT
TTTTTACAACTCTCCTATGAGTCGTTTACCCAGAATGCACAGGTACACTTGTTTAGAGGTAAATCCCCACTACCGCATTA
AGACCTCAGCGCGGCCGCAAATTTAAATAAAATGAAGTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCTATAGTGA
GTCGAATAAGGGCGACACAAAATTTATTCTAAATGCATAATAAATACTGATAACATCTTATAGTTTGTATTATATTTTGT
ATTATCGTTGACATGTATAATTTTGATATCAAAAACTGATTTTCCCTTTATTATTTTCGAGATTTATTTTCTTAATTCTC
TTTAACAAACTAGAAATATTGTATATACAAAAAATCATAAATAATAGATGAATAGTTTAATTATAGGTGTTCATCAATCG
AAAAAGCAACGTATCTTATTTAAAGTGCGTTGCTTTTTTCTCATTTATAAGGTTAAATAATTCTCATATATCAAGCAAAG
TGACAGGCGCCCTTAAATATTCTGACAAATGCTCTTTCCCTAAACTCCCCCCATAAAAAAACCCGCCGAAGCGGGTTTTT
ACGTTATTTGCGGATTAACGATTACTCGTTATCAGAACCGCCCAGGGGGCCCGAGCTTAAGACTGGCCGTCGTTTTACAA
CACAGAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTCCCTAC
TCTCGCCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG
GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA
CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG
ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGC
AGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGGCTAACTACGGCTACACTAGAAGAACAGTATTTGG
TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACG
GGGTCTGACGCTCAGTGGAACGACGCGCGCGTAACTCACGTTAAGGGATTTTGGTCATGAGCTTGCGCCGTCCCGTCAAG
TCAGCGTAATGCTCTGC
//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.  Shipping temperature is 2-8℃.