pFlag-CMV-6a
PVT10705 2ug
pFlag-CMV-6a Information
PFLAG-CMV-6a is a pCMV expression plasmid of mammalian cells.
pFlag-CMV-6a Multiple cloning site
pFlag-CMV-6a Sequence
LOCUS Exported 4697 bp ds-DNA circular SYN 06-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4697
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 318..697
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 698..901
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 936..959
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
misc_feature 963..1040
/label=MCS
polyA_signal 1038..1660
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
promoter 1689..2018
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1869..2004
/label=SV40 ori
/note="SV40 origin of replication"
rep_origin complement(2491..3079)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3250..4110)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4111..4215)
/gene="bla"
/label=AmpR promoter
rep_origin 4242..4697
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
901 tcgtttagtg aaccgtcaga attacgcgta ccatggacta caaagacgat gacgacaagc
961 tcaagcttgc ggccgcgaat tcagatctct gcaggatatc gtcgacggat ccggtaccat
1021 cgatagataa ttgacccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
1081 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
1141 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
1201 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
1261 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
1321 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
1381 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
1441 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
1501 ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc
1561 ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc
1621 tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc
1681 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
1741 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
1801 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
1861 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
1921 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
1981 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa
2041 accagaaagt taattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt
2101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
2161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
2221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
2281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
2341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
2401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
2461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
2521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
2581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
2641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
2701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
2761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
2881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
2941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
3001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
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