pFW5

Catalog No. PVT11374
Packing 2ug

pFW5 Information

Replicator: pij101pbr322rp4t

Plasmid classification: gene editing vector; Streptococcus suicidal plasmid

Prokaryotic resistance: spe

Clone strain: DH5a

Culture condition: 37℃

Plasmid host: broad host

pFW5 Sequence

tgtacaattgctagcgtacggccgtcgacgtccggatcctcgagctctagatcttaagcttgattttcgttcgtgaatac
atgttataataactataactaataacgtaacgtgactggcaagagatatttttaaaacaatgaataggtttacacttact
ttagttttatggaaatgaaagatcatatcatatataatctagaataaaattaactaaaataattattatctagataaaaa
atttagaagccaatgaaatctataaataaactaaattaagtttatttaattaacaactatggatataaaataggtactaa
tcaaaatagtgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaa
aataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagt
cgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggag
ataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaa
gaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaac
cataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattc
cattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactct
atattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggc
tgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggacta
atgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataaaaaaattgaaaaaatggtggaaac
acttttttcaatttttttgttttattatttaatatttgggaaatattcattctaattggtaagcgcgccatgggccctgc
agcatgcatatgcatccggagttcccgggcgccggcctaggactagtgatcaccggtcgctcttccgcttcctcgctcac
tgacgcgtaaaaaaggcccacaaaagtgggccttttttctcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaa
aggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcga
cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctc
tcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac
gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgc
tgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaa
caggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagga
cagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacc
accgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgat
cttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatct
tcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttac
caatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgta
gataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccag
atttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtccgataaacccagcgaaccatttgaggtga
taggtaagattataccgaggtatgaaaacgagaattggacctttacagaattactctatgaagcgccatatttaaaaagc
taccaagaaaaaaagcggccaaaaaggcctggtgccaccaggccaaaaaggccgctttttttcgaagaggatgaagagga
tgaggaggcagattgccttgaatatattgacaatactgataagataatatatcttttatatagaagattttcgttcgtga
atacat
//

Caution:
1.  This product is FOR RESEARCH USE ONLY!
2.  The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.  Shipping temperature is 2-8℃.