pGAPZB

Search name

pGAPZB,Plasmid pGAPZB,pGAPZB vector

 

pGAPZB Information

Bacterial Resistance:Zeo
Growth Strain:
Expression: Yeast
Use: Yeast expression

 

pGAPZB  Desciption

Glyceraldehyde -3- phosphate dehydrogenase (GAPDH) enzyme is stable and highly expressed in many biological cells including Pichia pastoris. Recently, the GAPDH protein coding gene promoter (GAP) has been studied in depth and can be expressed in high water in Pichia pastoris, depending on the use of carbon sources (Waterham et al., 1997). The expression level of GAP promoter (PGAP) was slightly higher than that of AOX1 promoter.

PGAPZ A, B, and C vectors (2.9 KB) and pGAPZ alpha, B, and C (3.1 KB) vectors were used to stably express recombinant proteins in the Pichia pastoris using a GAP promoter. The recombinant protein was expressed as a fusion protein with C- terminal myc epitope and C terminal His tag. In addition, the fusion protein pGAPZ alpha expresses at the N- end with the alpha factor secreting signal peptide of Saccharomyces cerevisiae. These two series of vectors provide three different reading frame carriers to facilitate cloning of vectors carrying C- terminal tags and / or N- terminal secretory signal peptides. These vectors are based on dominant selection markers, and Zeocin (bleomycin) screening markers can be used in Pichia pastoris and Escherichia coli simultaneously.

 

pGAPZB Sequence

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